Abstract: The present application discloses an epigenetic marker for colon cancer.

Abstract: A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.

Abstract: The present application discloses an epigenetic marker for lung cancer.

Abstract: The present application discloses an epigenetic marker for gastric cancer.

Abstract: The present application discloses a method for linear amplification of RNA.

Abstract: The present application discloses an epigenetic marker for colon cancer.

Abstract: The present application discloses a method for discovering a methylation marker gene for the conversion of a cell comprising: (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell; (ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and (iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene.

Abstract: Methylated promoters of colon cancer-specific genes and use thereof. Various disclosed aspects of the invention include methylated promoters of the colon cancer specific expression-decreased genes, microarrays for cancer diagnosis on which the methylated promoters are immobilized, and cancer diagnosis kits containing the methylated promoters. The methylated promoters of colon cancer-specific expression-decreased genes have utility for early detection of cancer and as targets for screening new drugs useful in the early treatment of cancer.

Abstract: A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.

Abstract: A method for the linear amplification of RNA using a high-heel primer, in which annealing and extension are performed at the same temperature using a high-heel primer. A DNA primer rather than an RNA primer is used, to provide a highly stable linear amplification process that can easily amplify even a small amount of sample, allowing a sufficient amount of target for microarray tests to be obtained from a nanogram level of total RNA by one linear amplification process using a high-heel primer. The inventive method shows a high correlation between RNAs of the same origin regardless of RNA amounts and purification methods, and efficiently achieves linear amplification within a short time at low cost as compared to prior methodology.