Abstract: The present disclosure relates to a gene editing system of Pseudomonas putida. The gene editing system of Pseudomonas putida includes a Pseudomonas putida, a RedCas expression plasmid and an exogenous gene expression plasmid. The RedCas expression plasmid includes a first replication origin, a first antibiotic resistance gene, a ?-Red expression cassette and a Cas expression cassette. The exogenous gene expression plasmid includes a second replication origin, a left homology arm, a second antibiotic resistance gene, an exogenous gene expression cassette, a right homology arm and a gRNA cassette.