Abstract: Provided is a method for detecting a target nucleic acid in a sample, comprising the following steps: reacting the sample with a mixed reaction system consisting of a sgRNA-Cas system and a recombinase-aid isothermal amplification system; and detecting a detectable signal generated by the reaction after the reaction is completed. The sgRNA-Cas system comprises a Cas12b protein and a sgRNA targeting the target nucleic acid; the recombinase-aid isothermal amplification system comprises a primer, a single-stranded DNA reporter molecule that generates the detectable signal after being cleaved. When a reagent user operates, a sample adding and mixing operation of stopping the reaction midway to add reaction component of the second step is leaved out, so that uniformity and coherence of the operation are better, facilitating the improvement of reaction precision. The operation of opening a reaction tube after the amplification reaction of recombinant polymerase is omitted.

Abstract: The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3? end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Abstract: The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3? end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Abstract: Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.

Abstract: Provided are a method and a device for detecting a genetic mutation, and a kit for typing genotypes of a pregnant woman and a fetus. The method comprises: performing high-throughput sequencing on free DNA in a pregnant woman's peripheral blood to obtain sequencing data; comparing the sequencing data with reference genome to obtain SNP sites; performing mixed genotyping on each SNP site to obtain target genotypes for each SNP site; and selecting a mutation site that causes the gene mutation from the genotype of the fetus in the target genotypes.

Abstract: The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3? end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.

Abstract: Provided is a method for constructing a DNA library for sequencing. The method comprises A-tailing of a single 3? end to at least a portion of blunt-end DNA fragments, and a step of blunt-end ligating the obtained DNA fragments having A tailing to the single 3? end. Also provided are a DNA library for sequencing constructed with the method and a corresponding sequencing method.

Abstract: Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.

Abstract: A kit, an apparatus, and a method for detecting chromosome aneuploidy.