Abstract: A DNA polymerase mutant and use thereof in sequencing. The DNA polymerase mutant includes at least three amino acid mutations at the following four sites or functionally equivalent sites, compared with a Pyrococcus sp. GB-D DNA polymerase exo-mutant: site 409, site 410, site 411, and site 486. The Pyrococcus sp. GB-D DNA polymerase exo-mutant has the amino acid sequence as set forth in SEQ ID NO: 1.

Abstract: A synthesis method for a second strand in DNB paired-end sequencing, a sequencing method and a related product. The synthesis method for the second strand comprises: after a first-strand sequencing on the DNB is completed, performing a second-strand synthesis using a second-strand synthesis primer, wherein the second-strand synthesis primer is a primer capable of being anchored on the surface of a sequencing chip.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: The present invention provides a nucleic acid loading reagent and a method for detecting the nucleic acid adsorption capacity of a sequencing chip using same. The method comprises at least one of the following conditions: (i) the nucleic acid loading reagent is at a pH value between 3 and 5; (ii) the nucleic acid loading reagent contains metal ions, the metal ions being, for example, from one or more of the following solutions: MgCl2, CeCl3, ZnCl2, MnCl2, CaCl2, CuCl2, and NiCl2; and (iii) the chip and the nucleic acid loading reagent are co-incubated for 10 min or above. The present invention provides a quality control scheme for the sequencing chip. The present invention provides a scheme capable of quickly optimizing the loading efficiency and loading bias of a nucleic acid library, and provides a reaction reagent capable of effectively improving the loading efficiency and loading bias of a nucleic acid library.

Abstract: The present invention provides a sequencing method based on a single fluorescent dye, in which a self-luminescence signal is used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of the polynucleotide sequence.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: The present invention provides a sequencing method based on a single fluorescent dye, in which a self-luminescence signal is used to distinguish the sequential incorporation of different nucleotides, thereby realizing the determination of the polynucleotide sequence.

Abstract: Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

Abstract: Provided is a synchronous sequencing method, including: constructing a sequencing library for a nucleic acid sample to be tested; loading the sequencing library onto a sequencing chip; performing a plurality of synchronous sequencing reaction cycles on the sequencing library, wherein an image set generated in each of the plurality of synchronous sequencing reaction cycles constitutes a raw image set of the synchronous sequencing; acquiring a base-calling result of the synchronous sequencing based on the raw image set of the synchronous sequencing; correcting the signal intensity value of each base channel based on a predetermined correction parameter to obtain a corrected base-calling result; and determining a base output result of the synchronous sequencing based on the corrected base-calling result.

Abstract: Provided are a rolling circle amplification method, a method for preparing a sequencing library, and a DNA nanoball prepared therefrom. The rolling circle amplification method includes: sequentially denaturing and annealing a double-stranded DNA and a mediating sequence in a same system, to complementarily pair the mediating sequence with two ends of a denatured single-stranded DNA; simultaneously introducing a ligase and a polymerase into the system to connect the two ends of the single-stranded DNA under action of the ligase; and performing a rolling circle amplification reaction under action of the polymerase by using the mediating sequence as a primer and the single-stranded DNA as a template, to obtain DNA nanoball.

Abstract: Provided are a multi-template nucleic acid synchronous sequencing method and use thereof. The method includes: providing a plurality of composite nucleic acid template spots, with a plurality of nucleic acid templates being arranged in the plurality of composite nucleic acid template spots; hybridizing the plurality of nucleic acid templates with corresponding sequencing primers thereof; performing, by use of the sequencing primers, a plurality of sequencing reaction cycles on each of the plurality of nucleic acid templates hybridized with the sequencing primers, wherein in each of the plurality of sequencing reaction cycles, signal intensities generated by the plurality of nucleic acid templates exhibit variations from one another; and classifying sequencing channel signals into the plurality of nucleic acid templates based on the variations in the signal intensities for each of the plurality of sequencing reaction cycles.

Abstract: Provided is a hydrogel-based sequencing method suitable for a flowcell sequencing system and also supporting open-source sequencing system, and which can effectively reduce costs while ensuring sequencing efficiency and accuracy.

Abstract: Provided is a method for sequencing a long-fragment nucleic acid. The nucleic acid molecules each containing a long insert, a first sequencing adapter, and a second sequencing adapter, is used to construct a sequencing library, and the sequencing is performed in segments to sequence the nucleic acids having the long inserts.

Abstract: The present invention provides a method for sequencing polynucleotides. Sequential incorporation of different nucleotides is detected by using the same light emitting signal, thereby achieving the determination of polynucleotide sequences.

Abstract: Provided are a reagent kit and use thereof in sequencing. The reagent kit includes a first reagent and a second reagent. The first reagent is selected from a disulfide bond reducing agent. The second reagent is selected from a blocking reagent suited to blocking a disulfide bond.

Abstract: The present invention relates to the field of nucleic acid sequencing. In particular, the present invention relates to a scanning reagent containing a saponin compound, a kit containing the scanning reagent and a method for nucleic acid sequencing by means of using the scanning reagent.

Abstract: The present disclosure relates to a method for analyzing the sequence of a target polynucleotide by polymerizing a nucleotide mixture and a polymerase multiple times to achieve full polymerization, thereby satisfying the requirements of sequencing, and improving read length and accuracy. Further, the present disclosure also relates to a reagent test kit, the reagent test kit being used for analyzing or sequencing polynucleotides.

Abstract: The present invention relates to a method for analyzing a sequence of a target polynucleotide. Full polymerization is achieved by means of multiple times of polymerization of a nucleotide mixture and polymerase, and a marker is detected while the polymerization reaction is performed. Furthermore, the present invention relates to a kit, capable of being used for analyzing or sequencing a polynucleotide.

Abstract: A nucleic acid probe and a nucleic acid sequencing method for performing sequencing while ligating nucleic acids. The nucleic acid probe is a DNA sequencing probe, comprising a first moiety, a second moiety, a linker, and a detectable label. A base of the first moiety is A, T, U, C, or G, a base of the second moiety is a random base and/or a universal base, and 3 bases or more are present in the second moiety. The first moiety and the second moiety are ligated via the linker, the connection between the first moiety and the ligation can be cleaved, and the detectable label is ligated to the second moiety or the linker. The above probe, a combination formed therewith, or a sequencing method using the same can reduce the number or types of probes in nucleic acid sequencing, thereby reducing cost.

Abstract: The present invention provides a method for sequencing a nucleic acid using an immersion reaction protocol. The immersion reaction protocol comprises sequentially immersing a solid support having nucleic acid molecules immobilized thereon in different reaction containers to realize nucleic acid sequencing. FIG.