Patents by Inventor Chongyi Chen

Chongyi Chen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes
    Patent number: 10894980
    Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: January 19, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Chongyi Chen, Dong Xing, Xiaoliang Sunney Xie
  • Methods of Amplifying Nucleic Acid Sequences
    Publication number: 20190002967
    Abstract: Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5? end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single strand
    Type: Application
    Filed: July 15, 2016
    Publication date: January 3, 2019
    Applicants: President and Fellows of Harvard College, President and Fellows of Harvard College
    Inventors: Chongyi Chen, Dong Xing, Xiaoliang Sunney Xie