Abstract: The present disclosure provides, among other things, systems, methods, and kits include internal amplification controls. Provided internal amplification controls are or include non-target sequences that are amplified during nucleic acid amplification. Provided internal amplification controls are linearly amplified. Provided internal amplification controls are useful for systems, methods and kits for nucleic acid amplification.

Abstract: A distributed blockchain system and method for storing, securing, and retrieving digital content, the system and method having a plurality of nodes designed to communicate over a network having one or more network devices. A control circuit assembly receives and transmits information via the network. A neural processing unit merges and unmerges digital content, the merged content viewable on at least one or more user interface and the unmerged content conditionally viewable from the merged content on the at least one or more user interface by way of one or more encrypted private keys. A computer-readable storage memory assembly, wherein the computer-readable storage memory assembly comprises a volatile memory storing a set of one or more computer-readable interactions, which when executed by the control circuit assembly cause the plurality of nodes to update the blockchain stored in a memory assembly based on communication with other nodes over the network assembly.

Abstract: Methods and apparatuses are described for nucleic acid analysis of environmental water samples and biological samples without the need for purification.

Abstract: The present disclosure provides, among other things, systems, methods, and kits include internal amplification controls. Provided internal amplification controls are or include non-target sequences that are amplified during nucleic acid amplification. Provided internal amplification controls are linearly amplified. Provided internal amplification controls are useful for systems, methods and kits for nucleic acid amplification.

Abstract: A fluid handling apparatus having a two-sided channel plate with channel ports extending from one side to the other. Fluid channels on both sides of the channel plate connected to the channel ports direct fluid through the channel plate in a desired fluid path, and the first face and the second face of the channel plate are sealed to fluidly encase the fluid channels.

Abstract: The present disclosure provides, among other things, systems for nucleic acid amplification. Provided systems for nucleic acid amplification substantially irreversibly seal the contents of a nucleic acid amplification reaction vessel. Provided systems reduce amplification contamination, for example amplification carryover contamination. Provided systems reduce amplification of spurious nucleic acids or amplification products. The present disclosure also provides methods of amplifying nucleic acids. The present disclosure also provides methods of amplifying nucleic acids using provided systems. Provided systems and methods are useful for nucleic acid amplification.

Abstract: The present disclosure relates to optical fiber-based devices, and more particularly to light-absorbing optical fiber-based systems and methods.

Abstract: The present disclosure provides, among other things, systems for nucleic acid amplification. Provided systems for nucleic acid amplification substantially irreversibly seal the contents of a nucleic acid amplification reaction vessel. Provided systems reduce amplification contamination, for example amplification carryover contamination. Provided systems reduce amplification of spurious nucleic acids or amplification products. The present disclosure also provides methods of amplifying nucleic acids. The present disclosure also provides methods of amplifying nucleic acids using provided systems. Provided systems and methods are useful for nucleic acid amplification.

Abstract: The present disclosure provides methods, systems, and apparatuses for collecting and/or amplifying nucleic acids. In general, provided methods, systems, and apparatuses involve contacting a sample including a nucleic acid with a nucleic acid amplification reagent without purification of nucleic acids from the sample.

Abstract: The thermal cycling system for performing a biological reaction at two or more different temperatures comprises: a) a heat source for setting at a fixed temperature; b) a reaction vessel containing material upon which the biological reaction is to be performed; c) mechanically-operable structure for altering the relative position of the heat source and the reaction vessel so that reaction vessel first achieves and maintains a desired first temperature in the reaction vessel for starting the carrying out of the biological reaction, and then for altering the relative position of the heat source and the reaction vessel so that the reaction vessel then achieves and maintains a second temperature for continuing the carrying out of the biological reaction on the biological material, and d) temperature-sensing structure operatively associated with the reaction vessel for controlling the altering of the relative position of the heat source and the reaction vessel so that the reaction vessel achieves and maintains the

Abstract: The present disclosure provides methods, systems, and apparatuses for collecting and/or amplifying nucleic acids. In general, provided methods, systems, and apparatuses involve contacting a sample including a nucleic acid with a nucleic acid amplification reagent without purification of nucleic acids from the sample.

Abstract: Methods and apparatus are described for nucleic acid analysis of swab samples without the need for purification.

Abstract: A reaction tube for use in performing the Polymerase Chain Reaction (PCR) in a thermal cycler, is provided herein such reaction tube includes a hollow tube of heat conducting synthetic plastics material having an open top and a flat bottom formed of an optically-clear material. Such reaction tube also includes a solid plug cap nesting within said hollow tube and to provide a minimized void volume within the hollow tube and to minimize evaporation and condensation of the biological fluid.

Abstract: Methods and apparatus are described for nucleic acid analysis of swab samples without the need for purification.

Abstract: A reaction tube for use in performing the Polymerase Chain Reaction (PCR) in a thermal cycler, is provided herein such reaction tube includes a hollow tube of heat conducting synthetic plastics material having an open top and a flat bottom formed of an optically-clear material. Such reaction tube also includes a solid plug cap nesting within said hollow tube and to provide a minimized void volume within the hollow tube and to minimize evaporation and condensation of the biological fluid.

Abstract: A thermal cycling system and method are provided herein, The thermal cycling system for performing a biological reaction at two or more different temperatures comprises: a) a heat source for setting at a fixed temperature; b) a reaction vessel containing material upon which the biological reaction is to be performed; c) mechanically-operable means for altering the relative position of the heat source and the reaction vessel so that reaction vessel first achieves and maintains a desired first temperature in the reaction vessel for starting the carrying out of the biological reaction, and then for altering the relative position of the heat source and the reaction vessel so that reaction vessel then achieves and maintains a second temperate for continuing the carrying out of the biological reaction on the biological material, and d) temperature-sensing means operatively associated with the reaction vessel for controlling the altering of the relative position of the heat source and the reaction vessel so that the