Abstract: The present invention is directed to tissue engineering and, more particularly, to devices and methods that are used to pattern or deposit cells to simulate a tissue type in two dimensions or in three dimensions or a cell migration device, a materials testing device for cell proliferation, migration or cell seeder for the Bioflex® flexible bottom culture plates or comparable culture plates. The present invention operates by providing negative pressure to create multiple troughs or indentations for cells to attach and grow on or in the Bioflex® flexible bottom culture plates. The present invention is also directed to a device and method for simulating a tissue wound using the above devices.