Patents by Inventor Chris Suh
Chris Suh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11274292Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: May 2, 2017Date of Patent: March 15, 2022Assignee: PHYNEXUS, INC.Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20210148925Abstract: The present invention relates to a method for qualitative analysis of a sample protein, which method comprises the steps of providing a water swollen gel column bed comprising bound protease and a sample protein in liquid buffer, digesting the sample protein into polypeptides by contact with the gel bed and subjecting the polypeptides to mass spectrometry (MS). The method is advantageously performed using back and forth flow of the sample protein including two or more repeats, and may be performed at a temperature of less than about 37° C., such as a temperature of less than about 30° C. The invention also includes an automated method as well as a device and a kit for performing mass-based analysis of proteins with higher speed than the prior art while maintaining conditions that are tolerable to the protease.Type: ApplicationFiled: April 29, 2019Publication date: May 20, 2021Applicant: Biotage ABInventors: Lee HOANG, Chris SUH, Douglas T. GJERDE
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Publication number: 20210087551Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: December 7, 2020Publication date: March 25, 2021Applicant: PHYNEXUS, INC.Inventors: Chris SUH, Carrie Loan Kim HUYNH, Lee HOANG, Douglas T. GJERDE, Jonathan Michael GRAMBOW
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Patent number: 10883100Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: April 9, 2017Date of Patent: January 5, 2021Assignee: PhyNexus, IncInventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Patent number: 10597652Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: GrantFiled: October 11, 2016Date of Patent: March 24, 2020Assignee: PHYNEXUS, INC.Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20200056168Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: May 2, 2017Publication date: February 20, 2020Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20190247846Abstract: An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation. In some embodiments, cells are purified from biological samples.Type: ApplicationFiled: February 9, 2018Publication date: August 15, 2019Inventors: Chris Suh, Douglas T. Gjerde, Lee Hoang, Mark Abel
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Patent number: 9891148Abstract: An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation. In some embodiments, cells are purified from biological samples.Type: GrantFiled: September 7, 2015Date of Patent: February 13, 2018Inventors: Douglas T. Gjerde, Lee Hoang, Chris Suh, Mark Abel
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Publication number: 20170233721Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: May 2, 2017Publication date: August 17, 2017Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20170211129Abstract: The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: April 9, 2017Publication date: July 27, 2017Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Publication number: 20170029809Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.Type: ApplicationFiled: October 11, 2016Publication date: February 2, 2017Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde, Jonathan Michael Grambow
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Patent number: 9328342Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: August 25, 2014Date of Patent: May 3, 2016Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde, Carrie Loan Kim Huynh
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Publication number: 20160061697Abstract: An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The pipette is hand-held when transferring between positions, e.g., between operation steps that take place in different rows of wells. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation. In some embodiments, cells are purified from biological samples.Type: ApplicationFiled: September 7, 2015Publication date: March 3, 2016Inventors: Douglas T. Gjerde, Lee Hoang, Chris Suh, Mark Abel
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Patent number: 9242244Abstract: An apparatus and method of using a pipette with pipette tip columns were developed in which a pipette is operated with the pipette tip columns inserted into the wells of a microplate. In this configuration the pipette is free standing and is essentially perpendicular to the microplate. The open lower ends of the pipette tip column are approximately centered within the plate well. The columns and plate are designed in such a way that the open lower ends of the pipette tip columns are in contact with liquid in the plate well however, the columns do not seal on the well bottom, preventing flow in and out of the column. The pipette contains the appropriate firmware and software to control flow for all steps of pipette tip column operation.Type: GrantFiled: February 8, 2011Date of Patent: January 26, 2016Inventors: Douglas T. Gjerde, Lee Hoang, Chris Suh, Mark Abel
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Publication number: 20150119563Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: ApplicationFiled: December 29, 2014Publication date: April 30, 2015Inventors: Chris Suh, Carrie Loan Kim Huynh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8921539Abstract: The invention provides pipette tip columns and automated methods for the purification of nucleic acids such as plasmids from unclarified cell lysates containing cell debris as well as methods for making and using such columns. The columns typically include a bed of medium positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: GrantFiled: March 29, 2012Date of Patent: December 30, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Publication number: 20140370590Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: ApplicationFiled: August 25, 2014Publication date: December 18, 2014Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde, Carrie Loan Kim Huynh
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Patent number: 8816064Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: August 26, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde
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Patent number: 8679857Abstract: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.Type: GrantFiled: January 28, 2013Date of Patent: March 25, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Jennifer E. Grant, Douglas T. Gjerde
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Patent number: 8664378Abstract: The present invention solves the problem of isolating nucleic acids from cells in the presence of the growth medium. The invention is particularly useful for isolating extrachromosomal replicons such as plasmids. Cells are lysed in the presence of the medium in which they were grown and nucleic acids are isolated using a pipette tip column. A liquid handling robot can be used to isolate nucleic acids from multiple samples simultaneously without the need for human intervention.Type: GrantFiled: September 30, 2011Date of Patent: March 4, 2014Assignee: PhyNexus, Inc.Inventors: Chris Suh, Lee Hoang, Douglas T. Gjerde