Abstract: The present disclosure relates to dibenzoxanthene compounds that are efficient quenchers of fluorescence, for example in the far red and near infrared spectrum. Applications using the dibenzoxanthene quenching compounds and methods of making same are also described.

Abstract: Disclosed is a method for determining the quality, expressed in terms of a quality value, of an RNA sample based on a multiplex fluorescent ratiometric method, wherein the ratio of small RNA to large and/or structured RNA in a sample is determined, thereby obtaining the quality value. Also disclosed is a method for determining the quality value of an RNA sample wherein the relationship of the intensity of the fluorescent signal corresponding to small RNA is compared to the intensity of the fluorescent signal corresponding to large and/or structured RNA in a sample. The relationship of the intensity of the two fluorescent signals is used to determine the amount of intact RNA present in the sample.

Abstract: Disclosed embodiments concern a phosphorus-containing heterocyclic compound according to the formula One exemplary compound is Certain embodiments fluoresce with excitation by light. The compounds are useful as fluorescent probes and as bioisosteres. In some embodiments, the compounds are bioisosteres of alpha-quinolones. Also disclosed is a method for making the compounds.

Abstract: Disclosed embodiments concern a phosphorus-containing heterocyclic compound. Certain embodiments, fluoresce with excitation by light. The compounds are useful as fluorescent probes and as bioisosteres. In some embodiments, the compounds are bioisosteres of carbostyils. Also disclosed is a method for making the compounds.