Abstract: Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

Abstract: Devices, kits, and methods are disclosed for use in detecting a concentration of an analyte of interest in a patient's liquid test sample. The devices, kits, and methods employ the use of one or more solid reagent zones that includes at least one analytical reagent for detection of an analyte of interest. The solid reagent zone(s) also includes at least one dye for determining whether results obtained from the diagnostic assay for the at least one analyte of interest are biased or inaccurate due to a loss of volume of a liquid reagent during the dispensing of the liquid reagent.

Abstract: Disclosed herein are methods for rapid detection of suspension of a blood sample from a subject and methods for improving the processing time of a point of care blood analysis. Specifically, the disclosed methods provide a rapid detection of hemoglobin concentration, more specifically glycated hemoglobin A1c, and determination of sugar concentration in the blood sample from the subject. Further disclosed herein are methods for rapidly diagnosing diabetes in a subject.

Abstract: Disclosed herein are methods of detecting ascorbic acid in a urine sample from a subject, including contacting at least a portion of the urine sample with a test strip including a reagent pad and detecting whether ascorbic acid is present in the urine sample by measuring an intensity of color on the reagent pad, wherein a reduction in the intensity of color on the reagent pad indicates the presence of ascorbic acid.

Abstract: Non-limiting embodiments of a modified devices that comprise at least one dye for determining whether results obtained from the conductance of at least one diagnostic assay are biased, as well as kits and methods of use related thereto.

Abstract: Devices and methods for the hook effect detection associated with analytes of interest in the conductance of one or more diagnostic assays, including, without limitation, immunoassays.

Abstract: Disclosed herein are methods of detecting ascorbic acid in a urine sample from a subject. The methods comprise contacting at least a portion of the urine sample with a test strip comprising a reagent pad and detecting whether ascorbic acid is present in the urine sample. The detecting comprises measuring an intensity of color on the reagent pad, wherein a reduction in the intensity of color on the reagent pad indicates the presence of ascorbic acid.

Abstract: Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

Abstract: Identifying a test product having a test region includes impinging on the test region a set of test signals at known test wavelengths. An image of the test region is generated from the reflected signals. The image is comprised of a set of reflectance values that are compared against reference reflectance values at the test signal wavelengths to determine the test product type.

Abstract: A system and method of correcting reflectance comprises determining a reflectance constant for a test product at a first wavelength for which reflectance does not substantially change with the presence of a test substance, with the test product loaded with the test substance, determining a reflectance at a second wavelength for which signal-to-noise ratio is maximized and determining a measured reflectance at the first wavelength, and determining a corrected reflectance as the product of the reflectance with a ratio of the reflectance constant to the measured reflectance.

Abstract: Biological fluid samples are deposited by methods that produce a uniform layer of the sample over a reagent-containing surface. In one embodiment, a nozzle having multiple openings is used to deposit a sample over the reagent-containing surface simultaneously. In an alternative embodiment, single droplets of the sample are deposited in a pattern on the surface, preferably in a sequence of parallel lines. The reaction between the biological sample and the reagents is read from a spectrographic image of the reagent-containing surface obtained by optical methods.

Abstract: An apparatus for verifying proper operation of an optical inspection machine, including a row of colored segments that simulate reagent pads containing known types of analytes at known concentrations positioned so that the row of colored segments can be illuminated by the readhead of the optical inspection machine. If the optical inspection machine provides results that correspond to the known types and concentrations of analytes, then the machine is operating properly.