Patents by Inventor Christian B. Anfinsen

Christian B. Anfinsen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Alpha-amylase from hyperthermophilic archaebacterium
    Patent number: 5578479
    Abstract: The present invention relates, in general, to .alpha.-amylase, and, in particular to .alpha.-amylase from a hyperthermophilic archaebacterium, wherein said .alpha.-amylase is in pure form. The invention also relates to a method of purifying a hyperthermophilic .alpha.-amylase.
    Type: Grant
    Filed: June 9, 1992
    Date of Patent: November 26, 1996
    Assignee: The Johns Hopkins University
    Inventors: Kenneth Laderman, Christian B. Anfinsen
  • Proteolytic enzymes from hyperthermophilic bacteria and processes for their production
    Patent number: 5391489
    Abstract: Cell-free extracts from Pyrococcus furiosus were found to possess unusually high levels of proteolytic activity as measured by hydrolysis of azocasein; loss in activity was only 30% after incubation for 24 hours at 98.degree. C. and the half-life of proteolytic activity at that temperature was about 60 hours. Furthermore, cell-free extracts incubated at 98.degree. C. in 1% sodium dodecyl sulfate (SDS) for 24 hours yielded an SDS-resistant protease having a temperature optimum of at least 100.degree. C. The enzyme retained at least 40% of its activity when tested at 98.degree. C. by azocasein hydrolysis in the presence of 4M urea, 2M guanidinium chloride, 10 mM dithiothreitol or 150 mM .beta.-mercaptoethanol. The protease was found to have a pH optimum of 6.8 at 98.degree. C. and retained more than 45% of its activity at pH 9.3 and 82% of its activity at pH 4.5 in assays performed at those values.
    Type: Grant
    Filed: June 30, 1993
    Date of Patent: February 21, 1995
    Assignee: Johns Hopkins University
    Inventors: Robert M. Kelly, Anne K. S. Robinson, Ilse I. Blumentals, Stephen H. Brown, Christian B. Anfinsen
  • .alpha.-amylase gene
    Patent number: 5366883
    Abstract: The present invention relates, in general, to a cloned .alpha.-amylase gene, and, in particular, to a cloned hyperthermophilic .alpha.-amylase gene and to methods of producing .alpha.-amylase using same.
    Type: Grant
    Filed: June 9, 1992
    Date of Patent: November 22, 1994
    Assignees: Takara Shuzo Co., Ltd., The Johns Hopkins University
    Inventors: Kiyozo Asada, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato, Kenneth Laderman, Christian B. Anfinsen
  • Proteolytic enzymes from hyperthermophilic bacteria and processes for their production
    Patent number: 5242817
    Abstract: Cell-free extracts from Pyrococcus furiosus were found to possess unusually high levels of proteolytic activity as measured by hydrolysis of azocasein; loss in activity was only 30% after incubation for 24 hours at 98.degree. C. and the half-life of proteolytic activity at that temperature was about 60 hours. Furthermore, cell-free extracts incubated at 98.degree. C. in 1% sodium dodecyl sulfate (SDS) for 24 hours yielded an SDS-resistant protease having a temperature optimum of at least 100.degree. C. The enzyme retained at least 40% of its activity when tested at 98.degree. C. by azocasein hydrolysis in the presence of 4M urea, 2M guanidinium chloride, 10 mM dithiothreitol or 150 mM .beta.-mercaptoethanol. The protease was found to have a pH optimum of 6.8 at 98.degree. C. and retained more than 45% of its activity at pH 9.3 and 82% of its activity at pH 4.5 in assays performed at those values.
    Type: Grant
    Filed: September 12, 1989
    Date of Patent: September 7, 1993
    Assignee: Johns Hopkins University
    Inventors: Robert M. Kelly, Anne K. S. Robinson, Ilse I. Blumentals, Stephen H. Brown, Christian B. Anfinsen