Abstract: A method for processing organic cells includes providing a microfluidic system having a chamber comprising a stationary phase in the form of a plurality of microparticles. The method further includes letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system. The method further includes accumulating the organic cells in a tapered section of the chamber that is upstream of a filter of the microfluidic system that is impermeable to the microparticles. The method further includes flushing a lysis agent into the chamber to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells.

Abstract: A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.

Abstract: A polymer layer system pressure sensor device includes a first polymer substrate having a first cavity and a first polymer membrane stretched over the first cavity. The first polymer membrane is configured to be deflected dependent on a pressure in the first cavity. The device further includes a first membrane metallization layer applied to the first polymer membrane above the first cavity. The first membrane metallization layer is configured to be deflected together with the first polymer membrane dependent on the pressure in the first cavity. The device further includes a second polymer substrate, arranged over the first polymer membrane, a second cavity, arranged over the first cavity, and a second polymer membrane, stretched over the second cavity. The device further includes a second membrane metallization layer applied to the second polymer membrane within the second cavity and includes a third polymer substrate arranged over the second polymer membrane.

Abstract: An apparatus for hermetically sealed storage of liquids for a microfluidic system includes at least one cavity and at least one sealing cone. A connection to the microfluidic system is established via the at least one sealing cone. Additionally, the at least one sealing cone is configured to close the at least one cavity.

Abstract: A film bag for a microfluidic analysis system includes a film bag bottom, a film bag opening arranged opposite the film bag bottom, and a peel seam arranged between the film bag bottom and the film bag opening. The peel seam is formed to produce a closed reagent receiving region between the peel seam and the film bag bottom and a tube between the peel seam and the film bag opening. The reagent receiving region has a reagent receiving length extending between the peel seam and the film bag bottom. The tube has a tube length extending between the peel seam and the film bag opening. The tube length is at least five percent of the reagent receiving length.

Abstract: An apparatus for purifying biological molecules, especially nucleic acids or proteins, includes at least one filter and a circuit. A method of purifying biological molecules using the apparatus includes pumping at least some fluids into the circuit via the filter. Fluid with biological cells is pumped into the circuit via the filter, and cells retained on the filter are digested. Binding buffer is pumped into the circuit via the filter in order to bind the biological molecules to the filter. A washing buffer is pumped into the circuit via the filter in order to clean the biological molecules bound to the filter so that the biological molecules bound to the filter are enabled for further use.

Abstract: An electronic sensor apparatus for detecting chemical or biological species includes a semiconductor chip, a sensor device, and a substrate. The chip is produced from a semiconductor substrate and is configured for one or more functions such as: amplifying and/or evaluating an electrical voltage, amplifying and/or evaluating an electric current, amplifying and/or evaluating an electrical charge, and amplifying and/or reading out capacitance changes. The sensor device has an active surface configured to detect chemical or biological species and generate an electrical signal based on a species-characteristic interaction with the active surface. The electrical signal can be an electrical voltage, an electric current, an electrical charge and/or a capacitance change. The substrate is produced from a melt-moldable material and has a surface including first and second regions. The chip is at least partly embedded in the first region, and the sensor device is at least partly embedded in the second region.

Abstract: A device, especially a microfluidic device for performance of an immunoassay, has a first, a second and a third fluidically connected chamber and a membrane. In the event of a given deflection of the membrane into the first chamber, a first fluid is passed at least partly out of the first chamber into the second chamber in such a way that a second fluid is at least partly displaced from the second chamber into the third chamber in such a way that the third chamber is entirely filled with the second fluid.

Abstract: A method for processing a sample of biological material containing target cells and companion cells in order to extract nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the companion cells from the sample. The method additionally includes decomposing the target cells by chemical or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method further includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acid of the target cells.

Abstract: A method for preparing a sample of biological materials containing target cells and accompanying cells for extracting nucleic acids of the target cells includes accumulating the target cells of the sample by separating the target cells or the accompanying cells from the sample. The method also includes digesting the target cells via chemical and/or physical lysis in order to produce a target cell lysate containing the nucleic acids of the target cells. The method furthermore includes purifying the nucleic acids from the target cell lysate in order to extract the nucleic acids of the target cells.

Abstract: A microfluidic device includes a first supply channel and a first discharge channel fluidically connected to one another by a first valve. The device also includes a second supply channel and a second discharge channel fluidically connected to one another by a second valve. At least one of the first channels can be fluidically connected to at least one of the second channels by a T-valve. The device also includes a control unit for controlling the valves, the control unit being configured in such a way that a first fluid from one of the first channels and a second fluid in one of the second channels can be laminated in parallel by actuating the valves in one of the first or second channels. A microfluidic system and a method for transporting fluids, and a use thereof are also disclosed.

Abstract: A method for processing organic cells includes providing a microfluidic system having a chamber comprising a stationary phase in the form of a plurality of microparticles. The method further includes letting a plurality of organic cells in a mobile phase into the chamber across a first opening of the microfluidic system. The method further includes accumulating the organic cells in a tapered section of the chamber that is upstream of a filter of the microfluidic system that is impermeable to the microparticles. The method further includes flushing a lysis agent into the chamber to resuspend the microparticles and the organic cells in the chamber for a disruption of the organic cells.

Abstract: A microfluidic device includes at least two layers arranged one above the other, a membrane which is arranged between the at least two layers, a cavity in one of the at least two layers, and a channel in the other of the at least two layers. The membrane is arranged so as to be expandable between the cavity and the channel. The membrane is expandable into at least one specified displacement volume.

Abstract: A microfluidic dielectrophoresis system includes: one supply device for a liquid medium having particles contained therein, N?2 microfluidic, dielectrophoretically active channels, which are equipped with electrodes, lines for the fluidic connection of the supply device to the channels, for the connection of the channels to one another, and for the drainage of the medium and/or the particles from the channels, and valves for setting the flow direction of the medium in the lines, the dielectrophoretically active channels being situated and being connected by lines in such a way that they may be operated connected in parallel and in series by switching the valves in relation to the flow direction of the medium and the electrodes of the various channels are activatable independently of one another.

Abstract: A microfluidic cell for the dielectrophoretic separation, accumulation, and/or lysis of polarizable bioparticles, including an interdigital electrode system composed of two electrode groups having interdigitated electrodes, and a micromixer having microchannels and microelevations. The interdigital electrode system and the micromixer are situated on the same side of the cell to improve the separation, accumulation, and/or lysis characteristics. Moreover, also described is a microfluidic system which includes such a microfluidic cell, and use thereof, and a method for separating, accumulating, and/or lysing polarizable bioparticles.

Abstract: A system for separating bodily fluid constituents is disclosed. The system includes a first substrate which, in a first main surface, has a first cavity for holding a bodily fluid sample, a second substrate, which, in a first main surface facing the first substrate, has a second cavity) for holding constituents separated from the bodily fluid sample, which second cavity lies opposite to the first cavity, a filter layer, which is arranged between the first substrate and the second substrate and which is designed to separate constituents of the bodily fluid sample in the first cavity and to pass the separated constituents to the second cavity, and a first thermoplastic connection layer, which is arranged between the first substrate and the filter layer, interconnects the first main surface of the first substrate and the filter layer in a fluid-tight manner and has a cutout lying opposite to the first cavity.

Abstract: A polymer layer system pressure sensor device includes a first polymer substrate having a first cavity and a first polymer membrane stretched over the first cavity. The first polymer membrane is configured to be deflected dependent on a pressure in the first cavity. The device further includes a first membrane metallization layer applied to the first polymer membrane above the first cavity. The first membrane metallization layer is configured to be deflected together with the first polymer membrane dependent on the pressure in the first cavity. The device further includes a second polymer substrate, arranged over the first polymer membrane, a second cavity, arranged over the first cavity, and a second polymer membrane, stretched over the second cavity. The device further includes a second membrane metallization layer applied to the second polymer membrane within the second cavity and includes a third polymer substrate arranged over the second polymer membrane.

Abstract: A microfluidic device includes at least two layers arranged one above the other, a membrane which is arranged between the at least two layers, a cavity in one of the at least two layers, and a channel in the other of the at least two layers. The membrane is arranged so as to be expandable between the cavity and the channel. The membrane is expandable into at least one specified displacement volume.

Abstract: A microfluidic peristaltic pump has an inflow channel, a first pumping chamber, which is operatively connected to the inflow channel by a first channel, and a first membrane. The pump also has a second pumping chamber, which is operatively connected to the inflow channel by a second channel, and a second membrane. A fluidic resistance of the first channel is different from a fluidic resistance of the second channel, and each channel is configured to realize a throttling function, such that the first and second membranes are deflected in a time sequence when a pressure is applied in the inflow channel, to realize a pumping function.

Abstract: A microfluidic dielectrophoresis system includes: one supply device for a liquid medium having particles contained therein, N?2 microfluidic, dielectrophoretically active channels, which are equipped with electrodes, lines for the fluidic connection of the supply device to the channels, for the connection of the channels to one another, and for the drainage of the medium and/or the particles from the channels, and valves for setting the flow direction of the medium in the lines, the dielectrophoretically active channels being situated and being connected by lines in such a way that they may be operated connected in parallel and in series by switching the valves in relation to the flow direction of the medium and the electrodes of the various channels are activatable independently of one another.