Abstract: Herein is reported a method for determining the binding interaction with a multimeric antigen of an antibody of the human IgG1 subclass that has at least two binding sites specifically binding to the antigen comprising the steps of 1) determining the binding affinity of the antibody for the multimeric antigen, and 2) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of Porphyromonas gingivalis under conditions and for a time sufficient to cleave the antibody into Fabs and Fc-region, and determining the binding affinity of the Fabs of the antibody for the multimeric, whereby the binding affinity of the antibody to the multimeric antigen to be affinity-driven if the binding affinity determined in both steps are comparable and to be avidity-driven if the binding affinity determined in both steps are different.

Abstract: The present invention relates to anti-VEGF/anti-IL-1beta antibodies and methods of using the same.

Abstract: The present invention generally relates to pH-dependent mutant interleukin-2 polypeptides that exhibit reduced IL-2 receptor binding at neutral pH and retained IL-2 receptor binding at reduced pH. In addition, the invention relates to immunoconjugates comprising said pH-dependent mutant IL-2 polypeptides, polynucleotide molecules encoding the pH-dependent mutant IL-2 polypeptides or immunoconjugates, and vectors and host cells comprising such polynucleotide molecules. The invention further relates to methods for producing the pH-dependent mutant IL-2 polypeptides or immunoconjugates, pharmaceutical compositions comprising the same, and uses thereof.

Abstract: Herein is reported the use of a binding assay of a bivalent, bispecific antibody that has the smaller kD value (dissociation constant) for the interaction with its antigen for the immobilization of the bivalent, bispecific antibody to a solid surface for the determination of the biological activity of the bivalent, bispecific antibody.

Abstract: The present invention relates to anti-VEGF/anti-IL-1beta antibodies and methods of using the same.

Abstract: A linear actuator includes: a guide rail assembly; a driving block sleeved on the guide rail assembly, where a top of the driving block is provided with a mounting surface, the mounting surface being disposed above the guide rail assembly and provided with a fixing structure and a prepositioning structure; and an electric motor, configured to actuate the driving block to slide back and forth along a length direction of the guide rail assembly.

Abstract: Herein is reported a method for determining the binding of an antibody, which comprises a first binding site specifically binding to a first antigen and a second binding site specifically binding to a second antigen, to said first and said second antigen, wherein the method comprises the steps of capturing the antibody on a solid phase using a capture reagent specifically binding to a constant domain of the antibody, incubating the captured antibody with the first or the second antigen to form a captured antibody-antigen complex and determining a first binding signal, either i) incubating the captured antibody-antigen complex with the antigen not used for the formation of the captured antibody-antigen complex to form a captured antibody-antigen-antigen complex and determining a second binding signal, or ii) regenerating the surface, capturing the antibody on a solid phase using a capture reagent specifically binding to a constant domain of the antibody, incubating the captured antibody with the antigen not us

Abstract: A control system for cinema seats and a method for controlling cinema seats, including: a plurality of seats; and an actuation device, configured for driving each of the seats to be folded or unfolded. The control system for cinema seats further includes: a control signal transmitter; control signal receivers, configured to be arranged corresponding to one or more of the seats; the control signal transmitter is configured to communicatively connect with each of the control signal receivers, the control signal receiver is electrically connected to one or more of the actuation devices, and each of the control signal receivers can retain a used record of the actuation device, and each of the control signal receivers receives a control signal from the control signal transmitter to control one or more of the actuation devices to drive one or more of the seats to be folded or unfolded.

Abstract: The present invention relates to anti-VEGF/anti-IL-1beta antibodies and methods of using the same.

Abstract: A linear actuator comprises a housing, inside which a spindle assembly, a push rod assembly driven by the spindle assembly, a limit switch for limiting stroke of the push rod assembly, and a motor for driving the spindle assembly are mounted. A connecting seat is disposed on the circuit board for centralizing connection terminals. The motor is electrically connected to the connection terminals. The housing is provided with a plug-in window aligned to the connecting seat. An electric plug of the linear actuator passes through the plug-in window and is plugged into the connecting seat. The electric plug and the housing are fastened together via a mounting component.

Abstract: An USB female socket, a button controller and a smart home. The USB socket may include a housing, a rubber core, and a set of terminals. The housing may define a cavity extending through a front end and a rear end of the housing. The set of terminals may include a first contact terminal and a plurality of second contact terminals. A front end of the first contact terminal may be located below a front end of a second contact terminal adjacent to the first contact terminal and thereby a variable gap may be formed between the front end of the first contact terminal and the front end of the adjacent second contact terminal.

Abstract: The present invention relates to anti-VEGF/anti-IL-1beta antibodies and methods of using the same.

Abstract: A driving block, a linear actuator and an adjusting apparatus are provided. The adjusting apparatus comprises an executing mechanism and a linear actuator. The linear actuator comprises a driving block and a spindle. The driving block comprises a base, a changeover unit, a fastener and a limit unit. The base is in transmission connection with the spindle. The changeover unit includes a first mounting portion, a second mounting portion and a connecting portion. The first mounting portion and the second mounting portion are oppositely disposed on the base and are both connected with connecting portion. The first mounting portion and the second mounting portion are connected to the base by the fastener. The connecting portion is engaged with the executing mechanism, and the limit unit is used to restrict movement of the changeover unit relative to the base.

Abstract: Herein is reported the use of a binding assay of a bivalent, bispecific antibody that has the smaller kD value (dissociation constant) for the interaction with its antigen for the immobilization of the bivalent, bispecific antibody to a solid surface for the determination of the biological activity of the bivalent, bispecific antibody.

Abstract: Herein is reported a method for determining the binding affinity of the binding sites of a bivalent full length antibody of the human IgG1 subclass to a homo-multimeric antigen comprising the steps of i) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of Porphyromonas gingivalis at a pH of from pH 7.5 to pH 8.5, in the presence of a reducing agent, at a temperature of from 30° C. to 42° C., for time of from 10 min. to 240 min. to cleave the antibody into Fabs and Fc-region, and ii) determining the binding affinity of the Fabs of the antibody for its antigen using a surface plasmon resonance method by directly applying the incubated reaction mixture obtained in the previous step in the surface plasmon resonance method and therewith determining the binding affinity of the binding sites of the bivalent full length antibody of the human IgG1 subclass.

Abstract: Herein is reported a method for determining the binding interaction with a multimeric antigen of an antibody of the human IgG1 subclass that has at least two binding sites specifically binding to the antigen comprising the steps of 1) determining the binding affinity of the antibody for the multimeric antigen, and 2) incubating a mixture comprising the antibody and a polypeptide that is derived from lysine-gingipain of Porphyromonas gingivalis under conditions and for a time sufficient to cleave the antibody into Fabs and Fc-region, and determining the binding affinity of the Fabs of the antibody for the multimeric, whereby the binding affinity of the antibody to the multimeric antigen to be affinity-driven if the binding affinity determined in both steps are comparable and to be avidity-driven if the binding affinity determined in both steps are different.

Abstract: A connecting cable (20), comprising: a first end (21) which is arranged with a first interface (23) and a second interface (24) coupled with each other, and a second end (22) which is arranged with a third interface (25) having a centrosymmetric cross section, wherein every two interfaces of the first, the second and the third interfaces (23, 24, 25) are in electrical communication, and the third interface (25) is connected with at least one of the first and the second interfaces (23, 24) by pluggable connection. When supplying driving circuits for actuators, each subsequent actuator can be parallelly connected through one connecting cable (20) with another connecting cable (20) of preceding actuator. In such a manner, disorders of cables in the fields can be avoided, whereby the risk of making mistakes during assembly and maintenance can be lowered. The cables can be neatly arranged in the fields.

Abstract: The present application relates to the technical field of smart home, especially to an USB socket, a button controller and a smart home. The USB socket includes housing, a rubber core, and a set of terminals. The housing defines a cavity for fixing the rubber core. The set of terminals includes a first contact terminal and a plurality of second contact terminals, and the first contact terminal and each of the second contact terminals are arranged side by side. A front end of each of the second contact terminals is exposed on the glue core and located within the cavity. A front end of the first contact terminal is located below the front end of its adjacent second contact terminal, and thereby an elastic gape is formed there between, such that when a USB plug is plugged into the cavity, the front end of the first contact terminal is contacted with the front end of the second contact terminal pressed by the USB plug.

Abstract: A slider for an actuator, the slider comprises a driving plate that moves under a driving force of a driving unit of the actuator, and a linkage lever channel arranged in the driving plate. A sliding track channel is disposed inside the driving plate, and the slider is sleeved on a sliding track of the actuator through the sliding track channel. When the slider moves along the sliding track of the actuator, a component to be driven is driven to move together. The slider for the actuator of the application has simple assembly process, convenient and quick assembly method, and can be applied to connecting different kinds of components to be driven to meet different user's requirements.