Patents by Inventor Christian Heinis
Christian Heinis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220267379Abstract: The present invention relates to a cyclic inhibitor of a kallikrein protease comprising or consisting of (I) the peptide (X1 (X2)(X3)R(X4)(X5)(X6)(X7)(X8)(X9)(X10)(X11) (Formula (X)), wherein (X1) is present or absent and, is preferably present, and if present, is an amino acid, and is most preferably D-alanine or G; (X2) is an amino acid with a side chain; (X3) is an amino acid with a polar uncharged side chain, is preferably with a polar uncharged side chain comprising a hydroxyl group, is more preferably T or S and is most preferably T; (X4) is an amino acid, preferably citrulline, Q or E; (X5) is an amino acid, preferably an amino acid with a hydrophobic side chain; (X6) is present or absent, is preferably absent, and, if present, is an amino acid with a negatively charged side chain, preferably D; (X7) is present or absent, is preferably present, and, if present, is an amino acid, more preferably an amino acid with a side chain comprising a pyrrole of indole, even more preferably P, hydroxyl-proline, (R)Type: ApplicationFiled: July 15, 2020Publication date: August 25, 2022Inventors: Christian Heinis, Patrick Gonschorek, Alessandro Zorzi, Alain Hovnanian, Olivier Gouin, Evgeniya Petrova
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Patent number: 9994617Abstract: The invention describes a method for selecting a polypeptide ligand having a desired level of specificity for a target, wherein the polypeptide ligand comprises a polypeptide comprising at least three reactive groups, separated by at least two loop sequences, and a molecular scaffold which forms covalent bonds with the reactive groups of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold, comprising (i) screening at least two different sets of polypeptide ligands against the target, and selecting one or more ligands from each library which interact with the target; (ii) comparing the activity of the selected ligands with one or more paralogues or orthologues of the target; and (iii) further selecting one or more ligands according to their activity towards said one or more paralogues or orthologues; wherein said two or more different sets of ligands differ in the length of the polypeptide loops formed on the molecular scaffold.Type: GrantFiled: October 8, 2012Date of Patent: June 12, 2018Assignee: BicycleRD LimitedInventors: John Tite, Edward Walker, Catherine Stace, Christian Heinis
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Publication number: 20180118786Abstract: The present invention relates to a bicyclic inhibitor of the coagulation enzyme activated factor XII (FXIIa) comprising or consisting of the peptide) (X1)(X2)(X3)n(X4)RL(X5)(X6)m(X7)(X9)l(X10)(X11)(X12)(X13)(X14)k(X15)(X16), wherein (X1) is present or absent and, if present, is an amino acid; (X2) is an amino acid with a side chain; (X3) is an amino acid and n is between 0 and 3, preferably 0 or 1 and most preferably 0; (X4) is an aliphatic L-amino acid or a cyclic L-amino acid, preferably L, P or an aromatic L-amino acid, and most preferably an aromatic L-amino acid; (X5) is an amino acid; (X6) is an amino acid and m is between 0 and 3, preferably 0 or 1 and most preferably 0; (X7) is an amino acid with a side chain; (X9) is an amino acid and l is between 0 and 3, preferably 0 or 1 and most preferably 0; (X10) is an amino acid; (X11) is an amino acid, preferably Q; (X12) is a hydrophobic L-amino acid, preferably an aliphatic L-amino acid, and is most preferably L; (X13) is an amino acid; (X14) is an amino acType: ApplicationFiled: April 27, 2016Publication date: May 3, 2018Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)Inventors: Christian HEINIS, Vanessa BAERISWYL, Simon MIDDENDORP, Jonas Alfred Karl WILBS
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Patent number: 9670484Abstract: The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.Type: GrantFiled: July 23, 2014Date of Patent: June 6, 2017Assignee: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory Paul Winter, Christian Heinis
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Patent number: 9670482Abstract: The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.Type: GrantFiled: November 26, 2013Date of Patent: June 6, 2017Assignee: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, John Tite, Marina Vaysburd, Daniel Paul Teufel, Lutz Riechmann
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Patent number: 9657288Abstract: The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.Type: GrantFiled: August 22, 2013Date of Patent: May 23, 2017Assignee: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory P. Winter, Christian Heinis
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Patent number: 9644201Abstract: The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.Type: GrantFiled: April 1, 2014Date of Patent: May 9, 2017Assignee: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Marina Vaysburd
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Publication number: 20170067045Abstract: The invention relates to a method for altering the conformational diversity of a first repertoire of polypeptide ligands, comprising a plurality of polypeptides comprising at least two reactive groups separated by a loop sequence covalently linked to a molecular scaffold which forms covalent bonds with said reactive groups, to produce a second repertoire of polypeptide ligands, comprising assembling said second repertoire from the polypeptides and structural scaffold of said first repertoire, incorporating one of the following alterations: (a) altering at least one reactive group; or (b) altering the nature of the molecular scaffold; or (c) altering the bond between at least one reactive group and the molecular scaffold; or (d) any combination of (a), (b) or (c).Type: ApplicationFiled: November 17, 2016Publication date: March 9, 2017Inventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Daniel Paul Teufel
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Patent number: 9518081Abstract: The invention relates to a method for altering the conformational diversity of a first repertoire of polypeptide ligands, comprising a plurality of polypeptides comprising at least two reactive groups separated by a loop sequence covalently linked to a molecular scaffold which forms covalent bonds with said reactive groups, to produce a second repertoire of polypeptide ligands, comprising assembling said second repertoire from the polypeptides and structural scaffold of said first repertoire, incorporating one of the following alterations: (a) altering at least one reactive group; or (b) altering the nature of the molecular scaffold; or (c) altering the bond between at least one reactive group and the molecular scaffold; or (d) any combination of (a), (b) or (c).Type: GrantFiled: August 12, 2010Date of Patent: December 13, 2016Assignee: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Daniel Paul Teufel
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Publication number: 20160046928Abstract: The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.Type: ApplicationFiled: April 1, 2014Publication date: February 18, 2016Inventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Marina Vaysburd
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Publication number: 20150166988Abstract: The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.Type: ApplicationFiled: July 23, 2014Publication date: June 18, 2015Inventors: Gregory Paul Winter, Christian Heinis
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Publication number: 20140249292Abstract: The invention describes a method for selecting a polypeptide ligand having a desired level of specificity for a target, wherein the polypeptide ligand comprises a polypeptide comprising at least three reactive groups, separated by at least two loop sequences, and a molecular scaffold which forms covalent bonds with the reactive groups of the polypeptide such that at least two polypeptide loops are formed on the molecular scaffold, comprising (i) screening at least two different sets of polypeptide ligands against the target, and selecting one or more ligands from each library which interact with the target; (ii) comparing the activity of the selected ligands with one or more paralogues or orthologues of the target; and (iii) further selecting one or more ligands according to their activity towards said one or more paralogues or orthologues; wherein said two or more different sets of ligands differ in the length of the polypeptide loops formed on the molecular scaffold.Type: ApplicationFiled: October 8, 2012Publication date: September 4, 2014Applicant: BICYCLE THERAPEUTICS LIMITEDInventors: John Tite, Edward Walker, Catherine Stace, Christian Heinis
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Patent number: 8778844Abstract: The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.Type: GrantFiled: February 4, 2010Date of Patent: July 15, 2014Assignee: Bicycle Therapeutics LimitedInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Marina Vaysburd
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Publication number: 20140187757Abstract: The invention relates to a complex comprising a phage particle, said phage particle comprising (i) a polypeptide; (ii) a nucleic acid encoding the polypeptide of (i); (iii) a connector compound attached to said polypeptide wherein said connector compound is attached to the polypeptide by at least three discrete covalent bonds. The invention also relates to libraries, and to methods for making complexes and to methods of screening using same.Type: ApplicationFiled: August 22, 2013Publication date: July 3, 2014Applicant: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory P. Winter, Christian Heinis
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Publication number: 20140163201Abstract: The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.Type: ApplicationFiled: November 26, 2013Publication date: June 12, 2014Applicant: BICYCLE THERAPEUTICS LIMITEDInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, John Tite, Marina Vaysburd, Daniel Paul Teufel, Lutz Riechmann
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Patent number: 8685890Abstract: The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.Type: GrantFiled: February 4, 2010Date of Patent: April 1, 2014Assignee: Bicycle Therapeutics LimitedInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, John Tite, Marina Vaysburd, Daniel Paul Teufel, Lutz Riechmann
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Publication number: 20120172235Abstract: The invention relates to a method for altering the conformational diversity of a first repertoire of polypeptide ligands, comprising a plurality of polypeptides comprising at least two reactive groups separated by a loop sequence covalently linked to a molecular scaffold which forms covalent bonds with said reactive groups, to produce a second repertoire of polypeptide ligands, comprising assembling said second repertoire from the polypeptides and structural scaffold of said first repertoire, incorporating one of the following alterations: (a) altering at least one reactive group; or (b) altering the nature of the molecular scaffold; or (c) altering the bond between at least one reactive group and the molecular scaffold; or (d) any combination of (a), (b) or (c).Type: ApplicationFiled: August 12, 2010Publication date: July 5, 2012Applicant: Medical Research CouncilInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Daniel Paul Teufel
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Publication number: 20120142541Abstract: The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.Type: ApplicationFiled: February 4, 2010Publication date: June 7, 2012Applicant: MEDICAL RESEARCH COUNCILInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, Marina Vaysburd
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Publication number: 20120101256Abstract: The invention relates to a method for providing a multispecific peptide ligand comprising a polypeptide covalently linked to a molecular scaffold at three or more amino acid residues and capable of binding to two or more separate targets, comprising the steps of: (a) providing a first repertoire of polypeptides, each polypeptide comprising two or more reactive groups capable of covalent linkage to a molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; (b) providing a second repertoire of polypeptides as described in (a); (c) joining at least one loop of one or more members of the first repertoire to at least one loop of one or more members of the second repertoire to form at least one polypeptide comprising two loops, and (d) conjugating the composite polypeptide(s) to a molecular scaffold at at least three amino acid positions.Type: ApplicationFiled: February 4, 2010Publication date: April 26, 2012Applicant: Medical Research CouncilInventors: Gregory Paul Winter, Christian Heinis, Elise Bernard, David Loakes, John Tite, Marina Vaysburd, Daniel Paul Teufel, Lutz Riechmann
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Publication number: 20120101253Abstract: The invention relates to a peptide ligand comprising a polypeptide linked to a molecular scaffold at n attachment points, wherein said polypeptide is cyclised and forms n separate loops subtended between said n attachment points on the molecular scaffold, wherein n is greater than or equal to 2.Type: ApplicationFiled: February 4, 2010Publication date: April 26, 2012Inventors: Christian Heinis, Jeremv Touati, Gregory Paul Winter