Abstract: A method for measuring interactions between labeled particles and ligands comprising the steps: a) providing a sample comprising labeled particles and ligands in a solution, wherein the labeled particles are dissolved or dispersed in the solution or are immobilized on a solid support; b) exciting fluorescently the labeled particles and detecting the fluorescence of the excited particles at a predetermined temperature; c) repeating steps (a) and (b) multiple times at different concentrations of the ligands in the solution; and d) determining the interaction between the labeled particles and the ligands based on the ligand concentration dependent change of the fluorescence of the labeled particles, wherein the labeled particles are labeled with one or more dyes.

Abstract: The present invention relates to a method for obtaining quantitative information on average donor-acceptor distance changes within a molecule or in between molecules using ensemble Förster resonance energy transfer (eFRET), and a measurement system comprising a controller adapted for performing the same, wherein the method comprises the steps of performing an eFRET measurement for at preferably a donor-, an acceptor-, and at least a donor-acceptor-labelled sample, wherein each sample comprises respective labelled molecule copies and wherein each eFRET measurement is performed using multiple respective labelled molecule copies, under a first and a second condition, correcting the obtained results for fluorophore-specific, condition-specific and inter-condition effects, determining condition-specific eFRET efficiencies based on the corrected results, and determining quantitative information on donor-acceptor distance changes within the molecule between the first and the second condition based on the respective

Abstract: A method for measuring interactions between labeled particles and ligands comprising the steps: a) providing a sample comprising labeled particles and ligands in a solution, wherein the labeled particles are dissolved or dispersed in the solution or are immobilized on a solid support; b) exciting fluorescently the labeled particles and detecting the fluorescence of the excited particles at a predetermined temperature; c) repeating steps (a) and (b) multiple times at different concentrations of the ligands in the solution; and d) determining the interaction between the labeled particles and the ligands based on the ligand concentration dependent change of the fluorescence of the labeled particles, wherein the labeled particles are labeled with one or more dyes.