Patents by Inventor Christian Paech
Christian Paech has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7396688Abstract: The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.Type: GrantFiled: December 14, 2004Date of Patent: July 8, 2008Assignee: Genencor International, Inc.Inventors: David A. Estell, Grant C. Ganshaw, Christian Paech, Sigrid Paech
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Patent number: 7329528Abstract: An enzyme multimer is provided which comprises a plurality of monomer units including a first monomer unit having enzymatic activity and a second monomer unit having enzymatic activity, wherein each of the first monomer unit and the second monomer unit comprise a cysteine residue and the cysteine residues of each monomer unit are covalently bound to each other through a disulfide bond to covalently attach the first monomer unit to the second monomer unit.Type: GrantFiled: December 22, 2004Date of Patent: February 12, 2008Assignee: Genencor International, Inc.Inventors: Richard L. Bott, Christian Paech, Shan Wu
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Patent number: 7157416Abstract: Enzyme-containing formulations having improved stability and enzymatic activity in liquid medium, comprising one or more protease enzymes produced from any Bacillus bacteria, at least about 5% alkali metal halide salt, and at least about 50% polyol. Also disclosed are methods for making such formulations.Type: GrantFiled: July 20, 2001Date of Patent: January 2, 2007Assignee: Genencor International, Inc.Inventors: Nathaniel T. Becker, Richard R. Bott, Shauna L. Bowden, Meng Hong Heng, Christian Paech, Antti V. Kosola
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Patent number: 7129076Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: GrantFiled: April 25, 2003Date of Patent: October 31, 2006Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20060084160Abstract: An enzyme multimer is provided which comprises a plurality of monomer units including a first monomer unit having enzymatic activity and a second monomer unit having enzymatic activity, wherein each of the first monomer unit and the second monomer unit comprise a cysteine residue and the cysteine residues of each monomer unit are covalently bound to each other through a disulfide bond to covalently attach the first monomer unit to the second monomer unit.Type: ApplicationFiled: December 22, 2004Publication date: April 20, 2006Inventors: Richard Bott, Christian Paech, Shan Wu
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Publication number: 20050244848Abstract: The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution.Type: ApplicationFiled: December 14, 2004Publication date: November 3, 2005Inventors: David Estell, Grant Ganshaw, Christian Paech, Sigrid Paech
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Patent number: 6946280Abstract: An enzyme multimer is provided which comprises a plurality of monomer units including a first monomer unit having enzymatic activity and a second monomer unit having enzymatic activity, wherein each of the first monomer unit and the second monomer unit comprise a cysteine residue and the cysteine residues of each monomer unit are covalently bound to each other through a disulfide bond to covalently attach the first monomer unit to the second monomer unit.Type: GrantFiled: March 29, 1996Date of Patent: September 20, 2005Assignee: Genencor International, Inc.Inventors: Richard L. Bott, Christian Paech, Shan Wu
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Patent number: 6927055Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: August 27, 2002Date of Patent: August 9, 2005Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Publication number: 20050170471Abstract: An enzyme multimer is provided which comprises a plurality of monomer units including a first monomer unit having enzymatic activity and a second monomer unit having enzymatic activity, wherein each of the first monomer unit and the second monomer unit comprise a cysteine residue and the cysteine residues of each monomer unit are covalently bound to each other through a disulfide bond to covalently attach the first monomer unit to the second monomer unit.Type: ApplicationFiled: February 16, 2005Publication date: August 4, 2005Inventors: Richard Bott, Christian Paech, Shan Wu
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Patent number: 6838425Abstract: The present invention relates to cleaning compositions comprising a protease variant.Type: GrantFiled: January 15, 2002Date of Patent: January 4, 2005Assignee: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Patent number: 6831053Abstract: The present invention relates to bleaching compositions comprising a protease variant.Type: GrantFiled: April 20, 2000Date of Patent: December 14, 2004Assignee: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Patent number: 6815193Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance.Type: GrantFiled: November 2, 2001Date of Patent: November 9, 2004Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, André C. Baeck
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Patent number: 6673590Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: GrantFiled: October 23, 1998Date of Patent: January 6, 2004Assignee: Genencor International, Inc.Inventors: Ayrookaran .J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030228995Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease.Type: ApplicationFiled: April 25, 2003Publication date: December 11, 2003Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030216277Abstract: Enzyme-containing formulations having improved stability and enzymatic activity in liquid medium, comprising one or more protease enzymes produced from any Bacillus bacteria, at least about 5% alkali metal halide salt, and at least about 50% polyol. Also disclosed are methods for making such formulations.Type: ApplicationFiled: January 16, 2003Publication date: November 20, 2003Inventors: Nathaniel T. Becker, Richard R. Bott, Shauna L. Bowden, Meng Hong Heng, Christian Paech, Antti V. Kosola
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Patent number: 6610642Abstract: The present invention relates to cleaning compositions comprising a protease variant.Type: GrantFiled: August 6, 2001Date of Patent: August 26, 2003Assignee: The Procter and Gamble CompanyInventors: Chanchal Kumar Ghosh, André Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030119690Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: ApplicationFiled: August 27, 2002Publication date: June 26, 2003Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, Andre C. Baeck
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Publication number: 20030078179Abstract: The present invention relates to cleaning compositions comprising a protease variant.Type: ApplicationFiled: January 15, 2002Publication date: April 24, 2003Applicant: The Procter & Gamble CompanyInventors: Chanchal Kumar Ghosh, Andre Cesar Baeck, Ryohei Ohtani, Alfred Busch, Michael Stanford Showell, Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell
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Publication number: 20030073222Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance.Type: ApplicationFiled: November 2, 2001Publication date: April 17, 2003Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, Andre C. Baeck
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Patent number: 6482628Abstract: Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.Type: GrantFiled: October 23, 1998Date of Patent: November 19, 2002Assignee: Genencor International, Inc.Inventors: Ayrookaran J. Poulose, Volker Schellenberger, James T. Kellis, Jr., Christian Paech, Joanne Nadherny, Donald P. Naki, Katherine D. Collier, Robert M. Caldwell, Andre C. Baeck