Patents by Inventor Christian Stemberger
Christian Stemberger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11913024Abstract: Provided herein are methods for culturing cells, including stimulating or expanding (proliferating), a plurality of cells in a composition of cells such as a population of lymphocytes. In some aspects, provided methods and reagents for the culturing, such as stimulation or expansion (proliferation), of cell populations involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for expansion or proliferation or other stimulation of a population of cells, and then such stimulatory agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.Type: GrantFiled: October 20, 2016Date of Patent: February 27, 2024Assignee: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger, Patricia Gräf
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Patent number: 11866465Abstract: Provided herein are oligomeric reagents, including oligomeric reagents of streptavidin or a streptavidin mutein, compositions thereof, and methods for manufacturing oligomeric reagents, including methods for reliably manufacturing oligomeric particle reagents of a desired size. In some cases, the reagents are oligomeric particle reagents containing a plurality of binding sites for agents, and thus the one or more agents are multimerized by reversibly binding to the oligomeric particle reagent, e.g., thereby creating a multimerized oligomeric particle reagent, having stimulatory agents multimerized thereon. Also provided are methods for using the oligomeric reagents for incubation or culturing, such as to induce stimulation of expansion, activation, and/or survival, of a composition of cells such as a population of lymphocytes.Type: GrantFiled: April 27, 2018Date of Patent: January 9, 2024Assignee: Juno Therapeutics GmbHInventors: Thomas Schmidt, Christian Stemberger, Tom Kowski, Ken Prentice
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Publication number: 20230295567Abstract: Provided herein are methods that relate, in some aspects, to the incubation or culturing, such as to induce stimulation of expansion (proliferation), activation, costimulation and/or survival, of a composition of cells, such as a population of lymphocytes. In some aspects, provided are methods and reagents for the stimulation, e.g., of expansion (proliferation), survival or persistence, activation, costimulation, or other effect, of cell populations that involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are reagents containing a plurality of binding sites for agents, such as multimerization reagents, and thus the one or more agents are multimerized by reversibly binding to the reagent, e.g., thereby creating a stimulatory reagent (multimerized agent), having stimulatory agents multimerized thereon.Type: ApplicationFiled: October 7, 2022Publication date: September 21, 2023Applicant: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger, Patricia Graf, Keenan Bashour
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Patent number: 11517627Abstract: Provided herein are cell surface conjugates containing a cell surface molecule and at least one agent, such as at least one affinity tag, and engineered cells expressing such cell surface conjugates. In some embodiments, the cell surface molecule does not contain an intracellular signaling domain or is not capable of mediating intracellular signaling. In some embodiments, the cells engineered to contain the cell surface conjugate, such as T cells, further contain a genetically engineered recombinant receptor that specifically binds to antigens, such as a chimeric antigen receptor (CAR). Also provided are methods of detecting, identifying, selecting or targeting cells expressing the cell surface conjugates, such as in connection with methods of manufacturing engineered cells or in connection with administration of such cells to subjects, including methods of adoptive cell therapy.Type: GrantFiled: January 19, 2018Date of Patent: December 6, 2022Assignee: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger
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Patent number: 11466253Abstract: Provided herein are methods that relate, in some aspects, to the incubation or culturing, such as to induce stimulation of expansion (proliferation), activation, costimulation and/or survival, of a composition of cells, such as a population of lymphocytes. In some aspects, provided are methods and reagents for the stimulation, e.g., of expansion (proliferation), survival or persistence, activation, costimulation, or other effect, of cell populations that involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are reagents containing a plurality of binding sites for agents, such as multimerization reagents, and thus the one or more agents are multimerized by reversibly binding to the reagent, e.g., thereby creating a stimulatory reagent (multimerized agent), having stimulatory agents multimerized thereon.Type: GrantFiled: October 20, 2016Date of Patent: October 11, 2022Assignee: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger, Patricia Gräf, Keenan Bashour
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Publication number: 20220195388Abstract: The present invention relates to in vitro-methods of expanding a population of cells such as lymphocytes, comprising contacting a sample comprising a population of cells with a multimerization reagent. The multimerization reagent has reversibly immobilized thereon (bound thereto) a first agent that provides a primary activation signal to the cells and optionally, a second agent that provides a co-stimulatory signal. The invention also provides multimerization reagents, kits, arrangements and an apparatus for expanding cells.Type: ApplicationFiled: March 11, 2022Publication date: June 23, 2022Applicant: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger
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Patent number: 11274278Abstract: The present invention relates to in vitro-methods of expanding a population of cells such as lymphocytes, comprising contacting a sample comprising a population of cells with a multimerization reagent. The multimerization reagent has reversibly immobilized thereon (bound thereto) a first agent that provides a primary activation signal to the cells and optionally, a second agent that provides a co-stimulatory signal. The invention also provides multimerization reagents, kits, arrangements and an apparatus for expanding cells.Type: GrantFiled: April 16, 2015Date of Patent: March 15, 2022Assignee: Juno Therapeutics GmbHInventors: Lothar Germeroth, Christian Stemberger
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Patent number: 9188589Abstract: The present invention relates to methods of treating a subject with a population of target cells defined by the presence of a specific receptor molecule on the surface; wherein the population of target cells is isolated by a method of reversibly staining the target cells with a detectable label.Type: GrantFiled: April 24, 2015Date of Patent: November 17, 2015Assignee: IBA GMBHInventors: Thomas Schmidt, Christian Stemberger, Dirk H. Busch, Lothar Germeroth
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Patent number: 9023604Abstract: The present invention relates to methods of reversibly staining a target cell. The invention also relates to methods of isolating a target cell or a target cell population that is defined by the presence of at least one common specific receptor molecule. The invention also provides kits that can be used to carry out the methods of the invention.Type: GrantFiled: July 17, 2012Date of Patent: May 5, 2015Assignee: IBA GmbHInventors: Thomas Schmidt, Christian Stemberger, Dirk H. Busch, Lothar Germeroth
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Publication number: 20140295458Abstract: The present invention relates to methods of reversibly staining a target cell. The invention also relates to methods of isolating a target cell or a target cell population that is defined by the presence of at least one common specific receptor molecule. The invention also provides kits that can be used to carry out the methods of the invention.Type: ApplicationFiled: July 17, 2012Publication date: October 2, 2014Applicant: IBA GMBHInventors: Thomas Schmidt, Christian Stemberger, Dirk H. Busch, Lothar Germeroth