Patents by Inventor Christina Chang
Christina Chang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240132961Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).Type: ApplicationFiled: October 22, 2023Publication date: April 25, 2024Inventors: Margaret Nakamoto, Eleen Shum, Nidhanjali BANSAL, Christina Chang
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Patent number: 11965208Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.Type: GrantFiled: April 15, 2020Date of Patent: April 23, 2024Assignee: Becton, Dickinson And CompanyInventors: Christina Chang, Sixing Li
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Patent number: 11834715Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).Type: GrantFiled: January 21, 2020Date of Patent: December 5, 2023Assignee: Becton, Dickinson and CompanyInventors: Margaret Nakamoto, Eleen Shum, Nidhanjali Bansal, Christina Chang
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Publication number: 20230348975Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: ApplicationFiled: March 21, 2023Publication date: November 2, 2023Inventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Publication number: 20230340566Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3? end and are subsequently barcoded on the 5? end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. There are provided, in some embodiments, methods of 5?-based and 3?-based gene expression profiling. Immune repertoire profiling methods are also provided in some embodiments.Type: ApplicationFiled: March 20, 2023Publication date: October 26, 2023Inventors: Christina Chang, Eleen Shum
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Patent number: 11773436Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3? end and are subsequently barcoded on the 5? end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. 5?- and/or 3?-barcoded nucleic acid targets can serve as templates for amplification reactions and/or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.Type: GrantFiled: November 6, 2020Date of Patent: October 3, 2023Assignee: Becton, Dickinson and CompanyInventors: Christina Chang, Margaret Nakamoto
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Patent number: 11661631Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: GrantFiled: January 21, 2020Date of Patent: May 30, 2023Assignee: Becton, Dickinson and CompanyInventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Patent number: 11639517Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3? end and are subsequently barcoded on the 5? end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. There are provided, in some embodiments, methods of 5?-based and 3?-based gene expression profiling. Immune repertoire profiling methods are also provided in some embodiments.Type: GrantFiled: September 30, 2019Date of Patent: May 2, 2023Assignee: Becton, Dickinson and CompanyInventors: Christina Chang, Eleen Shum
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Publication number: 20230125113Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.Type: ApplicationFiled: August 22, 2022Publication date: April 27, 2023Inventors: Christina Fan, Christina Chang, Eleen Shum, Olaf Zoellner, Nidhanjali Bansal, James Ghadiali, Gretchen Yinbon Lam, Eric Jensen, Jurg Rohrer, Brent Gaylord, Jody Martin, Jason G. Vidal
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Publication number: 20230057339Abstract: Systems, methods, and compositions for generating a high-resolution spatial map of a distribution of targets of a sample are described. Processes for generating the spatial map can include: receiving the sample at a substrate having a distribution of functionalized particles, each having a stochastic barcode sequence paired with a position on the substrate; promoting interactions between the distribution of targets of the sample and the distribution of functionalized particles upon transmitting heat to a surface of the substrate opposite the distribution of functionalized particles; applying a set of reactions to the sample at the substrate, obtaining a set of sequences of a population of molecules generated from the set of reactions, the set of sequences associated with the distribution of targets labeled using the stochastic barcode sequences of the distribution of functionalized particles, and returning a set of positions of the distribution of targets upon processing the set of sequences.Type: ApplicationFiled: August 25, 2022Publication date: February 23, 2023Inventors: Stephen P.A. Fodor, Hei Mun Christina Fan, Anaram Shahravan, Christina Chang, Jay Levine
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Publication number: 20220315988Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.Type: ApplicationFiled: June 2, 2022Publication date: October 6, 2022Inventors: Katherine Lazaruk, Eleen Shum, Christina Chang
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Patent number: 11460468Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.Type: GrantFiled: September 25, 2017Date of Patent: October 4, 2022Assignee: Becton, Dickinson and CompanyInventors: Christina Fan, Christina Chang, Eleen Shum, Olaf Zoellner, Nidhanjali Bansal, James Ghadiali, Gretchen Yinbon Lam, Eric Jensen, Jurg Rohrer, Brent Gaylord, Jody Martin, Jason G. Vidal
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Patent number: 11371076Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.Type: GrantFiled: January 15, 2020Date of Patent: June 28, 2022Assignee: Becton, Dickinson and CompanyInventors: Katherine Lazaruk, Eleen Shum, Christina Chang
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Publication number: 20210139970Abstract: Disclosed herein include systems, methods, compositions, and kits for labeling nucleic acid targets in a sample. In some embodiments, nucleic acid targets (e.g., mRNAs) are initially barcoded on the 3? end and are subsequently barcoded on the 5? end following a template switching reaction and intermolecular and/or intramolecular hybridization and extension. 5?- and/or 3?-barcoded nucleic acid targets can serve as templates for amplification reactions and/or random priming and extension reactions to generate a sequencing library. The method can comprise generating a full-length sequence of the nucleic acid target by aligning a plurality of sequencing reads. Immune repertoire profiling methods are also provided in some embodiments.Type: ApplicationFiled: November 6, 2020Publication date: May 13, 2021Inventors: Christina Chang, Margaret Nakamoto
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Publication number: 20200385780Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.Type: ApplicationFiled: April 14, 2020Publication date: December 10, 2020Inventors: Christina Chang, Christina Fan, Eleen Shum, Jody Martin, Nidhanjali Bansal, James Ghadiali, Katherine Lazaruk, Gretchen Yinbon Lam
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Publication number: 20200332351Abstract: Disclosed herein include systems, methods, compositions, and kits for associating single cell sequencing data with phenotypic data and/or agent exposure. In some embodiments, cell indexing oligonucleotides comprising cell indexing sequences are associated with cells. In some embodiments, synthetic particles are associated with stochastic labels comprising cell indexing sequences. Sequential hybridizing and imaging of probes annealing to a cell indexing sequence can generate a spatial index. The spatial index can be employed to identify of the partition from which a sequenced nucleic acid target molecule originated.Type: ApplicationFiled: April 15, 2020Publication date: October 22, 2020Inventors: Christina Chang, Sixing Li
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Publication number: 20200248263Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).Type: ApplicationFiled: January 21, 2020Publication date: August 6, 2020Inventors: Margaret Nakamoto, Eleen Shum, Nidhanjali BANSAL, Christina Chang
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Publication number: 20200232032Abstract: Disclosed herein include systems, methods, compositions, and kits for determining protein expression and gene expression simultaneously and for sample indexing. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) comprises one or more of a unique molecular label sequence, a primer adapter, antibody-specific barcode sequence, an alignment sequence, and/or a poly(A) sequence. In some embodiments, the oligonucleotide is associated with the cellular component-binding reagent via a linker (e.g., 5AmMC12).Type: ApplicationFiled: January 21, 2020Publication date: July 23, 2020Inventors: Eleen Shum, Katherine Lazaruk, Dennis E. Prosen, Devon Jensen, David Rosenfeld, Janice H. Lai, James Ghadiali, Christina Chang
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Publication number: 20200224247Abstract: Disclosed herein include systems, methods, compositions, and kits for PCR normalization. In some embodiments, after barcoding copies of a higher abundance target (e.g., a cDNA species), the barcoded copies are amplified using a pair of forward primers comprising one or more mismatches and a reverse primer. The amplified copies can be further linearly amplified using a forward primer comprising the sequence of one of the pair of forward primers, and a reverse primer.Type: ApplicationFiled: January 15, 2020Publication date: July 16, 2020Inventors: Katherine Lazaruk, Eleen Shum, Christina Chang
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Patent number: 10676779Abstract: Disclosed herein include systems, methods, compositions, and kits for sample identification. A sample indexing composition can comprise, for example, a protein binding reagent associated with a sample indexing oligonucleotide. Different sample indexing compositions can include sample indexing oligonucleotides with different sequences. Sample origin of cells can be identified based on the sequences of the sample indexing oligonucleotides. Sample indexing oligonucleotides can be barcoded using barcoded and lengthened using daisy-chaining primers.Type: GrantFiled: March 27, 2018Date of Patent: June 9, 2020Assignee: Becton, Dickinson and CompanyInventors: Christina Chang, Christina Fan, Eleen Shum, Jody Martin, Nidhanjali Bansal, James Ghadiali, Katherine Lazaruk, Gretchen Yinbon Lam