Abstract: The invention relates to hydrophobic patterned, low self-fluorescent substrates for attaching biomolecules and a method of making them. The patterned material will not interfere with end use assay analysis because the patterning composition is manufactured so to have very low self-fluorescence in the spectral regions typically used for microarraying applications. Further, the patterning compositions are chemically and physically durable and will not be deleteriously affected by typical chemical or physical processes utilized for cleaning, coating or assaying processes. In a preferred embodiment, the patterned silicone composition is applied to the substrate by screen-printing.