Abstract: The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' open reading frames of yjfA and ytfP are individually or jointly inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the yjfA and ytfP gene region; (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids; and (c) isolation of the L-amino acids.

Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The present invention relates to a fermentation process for the preparation of L-amino acids in which the following steps are carried out (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms' pckA gene, and/or the open reading frames of yjfA and ytfP are individually or jointed inactivated by one or methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or tranversion mutagenesis with incorporation of a non-sense mutation in the ytfP and ytfA gene, concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acids in the broth and E. coli, and isolation of the L-amino acids.

Abstract: The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out: a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which microorganisms at least the pckA gene and/or the open reading frames yjfA and ytfP are, individually or jointly, attenuated and, in particular, switched off, b) enrichment of the L-amino acid in the medium or in the bacterial cells, and c) isolation of the L-amino acid.

Abstract: The present invention relates to polynucleotides corresponding to the nadA gene and which encode the quinolinate synthetase A protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having quinolinate synthetase A activity.

Abstract: The present invention relates to polynucleotides corresponding to the nadC gene and which encode the nicotinate nucleotide pyrophosphorylase protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having nicotinate nucleotide pyrophosphorylase activity.

Abstract: The invention relates to polynucleotide sequences from coryneform bacteria coding for the ilvE gene and a process for the fermentative preparation of amino acids using bacteria in which the endogenous ilvE gene is enhanced, as well as to the use of polynucleotides containing the sequences according to the invention as hybridization probes.

Abstract: The invention relates to a fermentation process for the preparation of L-amino acids, especially L-threonine, in which the following steps are carried out:

Abstract: The present invention relates to polynucleotides corresponding to the nadA gene and which encode the quinolinate synthetase A protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having quinolinate synthetase A activity.

Abstract: The present invention relates to polynucleotides corresponding to the nadC gene and which encode the nicotinate nucleotide pyrophosphorylase protein, methods of producing nicotinic acid or nicotinic acid derivatives, and methods of screening for polynucleotides which encode proteins having nicotinate nucleotide pyrophosphorylase activity.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigM gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in attenuated form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the ndkA gene, and a host-vector system having a coryneform host bacterium in which the ndkA gene is present in attenuated form and a vector which carries at least the ndkA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes

Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the ppsA gene, and a host-vector system having a coryneform host bacterium in which the ppsA gene is present in attenuated form and a vector which carries at least the ppsA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.