Patents by Inventor Christine C. Stewart

Christine C. Stewart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Subtractive hybridization techniques for identifying differentially expressed and commonly expressed nucleic acid
    Patent number: 5935788
    Abstract: The present invention provides gene subtraction methods that can be used to identify and isolate from hybridization mixtures differentially expressed nucleic acid and/or commonly expressed nucleic acid between two populations of nucleic acid. The methods of the present invention employ a restriction endonuclease recognition site that is also recognized in whole or in part by a second restriction endonuclease (e.g., HinPI and BssHII or HhaI and HinP1I) to create different ends (e.g., sticky ends) between the first and second nucleic acid populations. In addition, the methods of the present invention use a selective ligation step, a biotinylated nucleotide extension step, or both to differentiate between homoduplexes, which allow for the identification of sequences that are different between the two nucleic acid populations, and heteroduplexes, which allow for the identification of sequences that are common to the two nucleic acid populations.
    Type: Grant
    Filed: July 24, 1997
    Date of Patent: August 10, 1999
    Assignee: Lifespan Biosciences, Inc.
    Inventors: Glenna C. Burmer, Joseph P. Brown, Christine C. Stewart