Patents by Inventor Christine Markert-Hahn
Christine Markert-Hahn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9868756Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.Type: GrantFiled: August 24, 2015Date of Patent: January 16, 2018Assignee: EPIGENOMICS AGInventors: Christine Markert-Hahn, Dirk Block
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Patent number: 9394332Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.Type: GrantFiled: August 25, 2003Date of Patent: July 19, 2016Assignee: Epigenomics AGInventors: Christine Markert-Hahn, Dirk Block
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Publication number: 20160068888Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.Type: ApplicationFiled: August 24, 2015Publication date: March 10, 2016Inventors: Christine Markert-Hahn, Dirk Block
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Patent number: 9267953Abstract: Methods for determining an analyte in a sample by immunoassay in a one-step format without performing washing steps are described. The method includes a first analyte-specific receptor that contains at least two binding sites for the analyte, and a second analyte-specific receptor that selectively binds to an aggregate arrangement of at least two analyte molecules bound to the first receptor.Type: GrantFiled: June 17, 2010Date of Patent: February 23, 2016Assignee: Roche Diagnostics Operations, Inc.Inventors: Barbara Upmeier, Ralf Bollhagen, Martina Bronold, Frederic Donie, Christine Markert-Hahn
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Patent number: 8664007Abstract: The invention concerns a method for determining antigen-specific antibodies of a particular immunoglobulin class in a sample by means of an immunoassay in an array format in which various binding partners Bnx are bound on different discrete areas on a support where Bnx in each case contain the various antigens that are able to specifically bind to the antibodies to be detected, by incubating the support with the sample and a binding partner B2 which carries a label and subsequently detecting the label on the respective discrete areas wherein B2 specifically binds antibodies of a certain immunoglobulin class that have been bound in an antigen-specific manner.Type: GrantFiled: October 28, 2005Date of Patent: March 4, 2014Assignee: Roche Diagnostics Operations, Inc.Inventors: Ursula Klause, Helmut Lenz, Christine Markert-Hahn
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Patent number: 8137937Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid via treatment of the solid phase-bound nucleic acid with bisulfite, desulfonation and elution of the nucleic acid from the solid phase.Type: GrantFiled: December 12, 2006Date of Patent: March 20, 2012Assignee: Roche Molecular Systems, Inc.Inventor: Christine Markert-Hahn
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Patent number: 8129106Abstract: A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.Type: GrantFiled: June 14, 2005Date of Patent: March 6, 2012Assignee: Max-Planck-Geselischaft zur Forderung der Wissenschaften E.V.Inventors: Elmar Weinhold, Thomas Meier, Hartmut Düfel, Christine Markert-Hahn, Rainer Schmuck
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Patent number: 7989169Abstract: The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.Type: GrantFiled: September 2, 2005Date of Patent: August 2, 2011Assignee: Roche Molecular Systems, Inc.Inventors: Frank Bergmann, Christine Markert-Hahn
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Publication number: 20110165564Abstract: A method for detecting sequence specific methylation in a biomolecule, comprising: (a) contacting the biomolecule with an S-adenosyl-L-methionine-dependent methyltransferase in the presence of a detectable cofactor of said methyltransferase; and (b) detecting whether the recognition sequence of said methyltransferase has been modified with the cofactor or a derivative thereof, wherein modification of the recognition sequence of said methyltransferase is indicative of an absence of methylation at said recognition sequence. Also disclosed is a cofactor specific for S-adenosyl-L-methionine-dependent methyltransferases, wherein said cofactor is an N-adenosylaziridine derivative with a reporter group attached to the 6 or 7 position of the adenine ring or attached to the aziridine ring. A complex of the cofactor and a methyltransferase a composition comprising the cofactor or the complex and the use of the cofactor or the complex for detecting sequence-specific methylation in DNA molecules are also disclosed.Type: ApplicationFiled: June 14, 2005Publication date: July 7, 2011Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.Inventors: Elmar Weinhold, Thomas Meier, Hartmut Düfel, Christine Markert-Hahn, Rainer Schmuck
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Publication number: 20110143454Abstract: The invention relates to a method for determining an analyte in a sample by means of an assay that can be carried out in a one-step format without performing washing steps. The method includes a first analyte-specific receptor that contains at least one bonding point for the analyte, e.g., several antibodies on a particle, as well as a second analyte-specific receptor that can selectively bond to an arrangement comprising at least two analyte molecules which are bound to the first receptor. An antibody sandwich immune assay is described in which the detection is based on electrochemiluminescence.Type: ApplicationFiled: June 17, 2010Publication date: June 16, 2011Inventors: Barbara Upmeier, Ralf Bollhagen, Martina Bronold, Frederic Donie, Christine Markert-Hahn
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Publication number: 20090208930Abstract: The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.Type: ApplicationFiled: September 2, 2005Publication date: August 20, 2009Inventors: Frank Bergmann, Christine Markert-Hahn
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Patent number: 7501240Abstract: The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.Type: GrantFiled: December 1, 2004Date of Patent: March 10, 2009Assignee: Roche Molecular Systems, Inc.Inventors: Christian Birkner, Christine Markert-Hahn, Herbert von der Eltz
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Publication number: 20080281087Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines. In this method, the nucleic acid is bisulfite treated and is bound to a solid phase when an alkaline solution is added for desulfonation and elution of the nucleic acid from the solid phase. The nucleic acid can be amplified in an additional step. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, several uses of the alkaline solution for bisulfite treatment are disclosed and a kit containing a bisulfite reagent, a solid phase and an alkaline solution.Type: ApplicationFiled: December 12, 2006Publication date: November 13, 2008Applicant: Roche Molecular Systems, Inc. Patent DepartmentInventor: Christine Markert-Hahn
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Patent number: 7413855Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.Type: GrantFiled: January 28, 2004Date of Patent: August 19, 2008Assignee: Roche Molecular Systems, Inc.Inventors: Frank Bergmann, Christine Markert-Hahn, Joerg Kleibler, Dirk Block
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Publication number: 20070190530Abstract: The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.Type: ApplicationFiled: December 1, 2004Publication date: August 16, 2007Inventors: Christian Birkner, Christine Markert-Hahn, Herbert von der Eltz
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Publication number: 20060115907Abstract: The invention concerns a method for determining antigen-specific antibodies of a particular immunoglobulin class in a sample by means of an immunoassay in an array format in which various binding partners Bnx are bound on different discrete areas on a support where Bnx in each case contain the various antigens that are able to specifically bind to the antibodies to be detected, by incubating the support with the sample and a binding partner B2 which carries a label and subsequently detecting the label on the respective discrete areas wherein B2 specifically binds antibodies of a certain immunoglobulin class that have been bound in an antigen-specific manner.Type: ApplicationFiled: October 28, 2005Publication date: June 1, 2006Inventors: Ursula Klause, Helmut Lenz, Christine Markert-Hahn
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Publication number: 20040241704Abstract: The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is bound to a solid phase during the deamination and/or desulfonation step of the bisulfite reaction. The solid phase is preferably a material comprising glass or silica, more preferably a glass fleece, glass membrane or a magnetic glass particle. Further, the use of a solid phase for binding a nucleic acid during the deamination and/or desulfonation step of the bisulfite reaction is disclosed and a kit containing a bisulfite reagent and a solid phase.Type: ApplicationFiled: August 25, 2003Publication date: December 2, 2004Applicant: ROCHE MOLECULAR SYSTEMS, INCInventors: Christine Markert-Hahn, Dirk Block
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Publication number: 20030199078Abstract: The invention concerns a process for preparing biological samples for the subsequent detection of an analyte. In particular, the invention relates to a process for the isolation of a nucleic acid in a sample using a suspension of magnetic glass particles. In addition, kits and apparatuses containing magnetic glass particles for sample preparation are provided.Type: ApplicationFiled: April 30, 2003Publication date: October 23, 2003Inventors: Jorg Kleiber, Christine Markert-Hahn, Herbert Harttig
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Patent number: 6562568Abstract: The invention concerns a process for preparing biological samples for the subsequent detection of an analyte. In particular, the invention relates to a process for the isolation of a nucleic acid in a sample using a suspension of magnetic glass particles. In addition, kits and apparatuses containing magnetic glass particles for sample preparation are provided.Type: GrantFiled: September 5, 2000Date of Patent: May 13, 2003Assignee: Roche Diagnostics GmbHInventors: Jörg Kleiber, Christine Markert-Hahn, Herbert Harttig
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Patent number: 5601824Abstract: Compound of the general formula ##STR1## wherein R is an ester-activating group, m and n are identical or different and 0-6, p =0, 2-4, and q and s=1, p=0 if s=0 and p=2-4 if s=1. The compound is novel and suitable for the reproducible coupling of immunological substances at a high yield.Type: GrantFiled: February 23, 1996Date of Patent: February 11, 1997Assignee: Boehringer Mannheim GmbHInventors: Eva Hoess, Erasmus Huber, Christine Markert-Hahn, Beatus Ofenloch-Haehnle