Abstract: The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.

Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.

Abstract: The present invention relates to a method of obtaining peptides from procaryotic and/or eucaryotic cells.

Abstract: Disclosed herein is a device for sampling blood by capillarity for separating a plasma phase of the blood from the cell phase. The device is a strip and includes a filter having a membrane with no clumping agents, a prefilter for spreading blood over the surface of the membrane; and an absorbent paper downstream from the filter. The membrane is dimensioned to cover only a part of the surface of the prefilter and the migration of blood by capillarity within the prefilter takes place essentially lengthwise relative to the strip before blood comes into contact with the membrane.

Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

Abstract: It relates to a method for amplifying at least one nucleotide molecule contained in a sample (E) comprising the following successive steps consisting of (a) subjecting said sample (E) to a 1st amplification step on an area (Z1) of a support; (b) bringing by electrowetting at least one portion of the sample (Ea) obtained after step (a) from the area (Z1) onto at least one area (Z2) of said support, distinct from the area (Z1); (c) subjecting the sample (Ea) to a 2nd amplification step on said area (Z2). It also relates to a device which may be applied within the scope of said method.

Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

Abstract: The invention relates to a device for taking blood by capillarity and incorporating a filter and downstream therefrom, an absorbent material for separating a plasma phase of the blood from a cell phase. The device is characterized in that the filter comprises in succession: a prefilter (6) for spreading the blood over the surface of a first membrane (2) having pores of dimension lying in the range 0.1 ?m to 5 ?m, said membrane having no clumping agent; and said first membrane (2).

Abstract: Provided is a microfluidic device comprising a microfluidic substrate comprising at least one pathway for sample flow; and at least one thermal transfer member which is capable of cycling between at least two temperatures. The thermal transfer member is adapted to heat at least a portion of the sample pathway while a sample is flowing along said at least a portion of said sample pathway. Provided also are methods of carrying out biochemical protocols using such a device.

Abstract: Provided is a microfluidic device comprising a microfluidic substrate comprising at least one pathway for sample flow; and at least one thermal transfer member which is capable of cycling between at least two temperatures. The thermal transfer member is adapted to heat at least a portion of the sample pathway while a sample is flowing along said at least a portion of said sample pathway. Provided also are methods of carrying out biochemical protocols using such a device.

Abstract: Devices and methods for carrying out a chemical or biochemical protocol are disclosed. In one embodiment, the chemical or biochemical protocol is performed by cycling at least one thermal transfer member between at least two temperatures while liquid samples on which the chemical or biochemical protocol is to be performed are continuously moving through at least one temperature regulated zone upon which the at least one thermal transfer member acts. In some embodiments, the device comprises a sample transport member that comprises liquid samples in sample receiving regions. The sample transport member moves the samples continuously through a temperature regulated zone which cycles between at least two temperatures while the liquid samples are moving through a temperature regulated zone on which at least one thermal transfer member acts. In some embodiments, the sample receiving regions comprise wells, hydrophillic films or hydrophillic filaments.

Abstract: Devices and methods for carrying out a chemical or biochemical protocol are disclosed. In one embodiment, the chemical or biochemical protocol is performed by cycling at least one thermal transfer member between at least two temperatures while liquid samples on which the chemical or biochemical protocol is to be performed are continuously moving through at least one temperature regulated zone upon which the at least one thermal transfer member acts. In some embodiments, the device comprises a sample transport member that comprises liquid samples in sample receiving regions. The sample transport member moves the samples continuously through a temperature regulated zone which cycles between at least two temperatures while the liquid samples are moving through a temperature regulated zone on which at least one thermal transfer member acts. In some embodiments, the sample receiving regions comprise wells, hydrophillic films or hydrophillic filaments.

Abstract: The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.

Abstract: Methods, devices, libraries, kits and systems for detecting nucleic acids are provided. Universal libraries of standard and modified nucleic acids are provided for nucleic acid detection, as are related methods.

Abstract: The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.

Abstract: Devices and methods for carrying out a chemical or biochemical protocol are disclosed. In one embodiment, the chemical or biochemical protocol is performed by cycling at least one thermal transfer member between at least two temperatures while liquid samples on which the chemical or biochemical protocol is to be performed are continuously moving through at least one temperature regulated zone upon which the at least one thermal transfer member acts. In some embodiments, the device comprises a sample transport member that comprises liquid samples in sample receiving regions. The sample transport member moves the samples continuously through a temperature regulated zone which cycles between at least two temperatures while the liquid samples are moving through a temperature regulated zone on which at least one thermal transfer member acts. In some embodiments, the sample receiving regions comprise wells, hydrophillic films or hydrophillic filaments.

Abstract: Methods for amplifying and sequencing nucleic acids are disclosed. In addition, supports for the solid phase amplification or sequencing of nucleic acids are disclosed. The supports comprise a solid support, a linker arm having functional groups capable of binding to the solid support, and an oligonucleotide bound to the linker arm.

Abstract: A support for solid phase amplification or sequencing of nucleic acids has a functionalized solid support, a linker arm having functional groups covalently bound to the solid support through at least one binding site of the functional groups, and an oligonucleotide primer bound at its 5′ end to the linker, the primer, thereby, being immobilized on the solid support.

Abstract: The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.