Abstract: The disclosure relates to a device for dielectrophoretic capture of particles. The device includes at least one electrical contact and at least one layer. The at least one layer includes a top layer side, a bottom layer side, and a barrier structure. The barrier structure is configured such that a fluid comprising the particles can flow through the barrier structure which is disposed on the top layer side. The barrier structure spaces the top layer side apart from the bottom layer side of at least one of the same layer and a second of the at least one layer.

Abstract: A reaction vessel for a diagnostic analyzer comprising a plurality of processing stations. The reaction vessel comprises at least one sensor configured to measure at least one physical parameter associated with at least one of the processing stations of the diagnostic analyzer when disposed at the at least one of the processing stations, a memory configured to at least temporarily store at least one measurement value indicating the physical parameter provided by the sensor, a processing unit configured to control the sensor and to output measurement data including the measurement value from the memory, an interface configured to provide communication of the processing unit with an external electronic device, a power source configured to supply electric power to the sensor, the processing unit and the memory. The reaction vessel defines an internal volume. The sensor, the processing unit, the memory and the interface are arranged within the internal volume.

Abstract: The disclosure relates to a method for operating a quantitative polymerase chain reaction (qPCR) method, having the following steps: cyclically carrying out qPCR cycles; measuring the fluorescence in each qPCR cycle in order to obtain a qPCR curve of intensity values; determining the reaction efficiency (?) for each cycle; correcting the intensity value of each cycle on the basis of the reaction efficiency (?) determined for the cycle in question in order to obtain a corrected qPCR curve; and operating the qPCR method on the basis of the corrected qPCR curve.

Abstract: The disclosure relates to a method for carrying out a quantitative polymerase chain reaction (qPCR) process, comprising the following steps:—cyclically carrying out qPCR cycles; —measuring the fluorescence for each qPCR cycle to obtain a qPCR curve of intensity values (I); —creating a probability density function (PDF) from the intensity values (I); —establishing a presence or absence of the DNA strand section to be detected depending on the presence of one or more features of the probability density function (PDF); —carrying out the qPCR process depending on the presence or absence of the DNA strand section to be detected.

Abstract: The disclosure relates to a computer-implemented method for carrying out a quantitative polymerase chain reaction (qPCR) process, comprising the following steps: —cyclically carrying out qPCR cycles; —measuring an intensity value of a fluorescence relating to each qPCR cycle to obtain a qPCR curve from intensity values; —analyzing the shape of the qPCR curve using a data-based classification model trained to provide a classification result depending on the shape of the qPCR curve; and—carrying out the qPCR process depending on the classification result of the analysis of the shape of the qPCR curve.

Abstract: The disclosure relates to a method for carrying out a quantitative polymerase chain reaction (qPCR) including cyclically executing a predetermined plurality of qPCR cycles, and measuring an intensity value after each of the predetermined plurality of qPCR cycles to obtain a measured portion of a qPCR curve of intensity values. The method includes estimating, after cycling the predetermined plurality of qPCR cycles, a remainder of the qPCR curve using the measured intensity values and a data-based trainable qPCR model, and selecting one of a plurality of steps of the method based on the remainder of the plot of the qPCR curve. The method includes conducting the selected one of the plurality of steps of the method.

Abstract: The disclosure relates to a device for dielectrophoretic capture of particles. The device includes at least one electrical contact and at least one layer. The at least one layer includes a top layer side, a bottom layer side, and a barrier structure. The barrier structure is configured such that a fluid comprising the particles can flow through the barrier structure which is disposed on the top layer side. The barrier structure spaces the top layer side apart from the bottom layer side of at least one of the same layer and a second of the at least one layer.

Abstract: A system for introducing sample material includes a microfluidic chip and a sample feeding zone disposed on or in the microfluidic chip. The sample feeding zone is configured to introduce the sample material into the microfluidic chip.