Abstract: A method for operating a scanning microscope and for determining point spread functions, with which sample images are recorded with the scanning microscope. The method includes scanning a sample with at least one illuminating light beam; recording at least one sample image with a detector device during a scan by the illuminating light beam; and comprising the point spread function, with which a sample image is recorded, from the at least one sample image. A detector device having receiving elements is used, where the distance between the receiving elements is smaller than a diffraction disk that generates a sample point on the detector device. Detector signals, generated by means of the receiving elements, are read out for each of the different positions of the illuminating light beam on the sample, as a result of which the scanning of the sample allows the detector signals, which are read out, to generate a plurality of sample images.

Abstract: An optical detection filter has a transmission spectrum for detecting fluorescence light of a plurality of different fluorescent dyes. In the range between 350 nm and 1000 nm, the transmission spectrum has a first stopband (DS1) from D?1 to D?2, a first passband (DD1) from D?2 to D?3, a second stopband (DS2) from D?3 to D?4, a second passband (DD2) from D?4 to D?5, a third stopband (DS3) from D?5 to D?6, and a third passband (DD3) from D?6 to D?7. The stopbands (DS1, DS2, DS3) each have a mean transmittance of at most 0.01, typically at most 0.001 or at most 0.0001, and the passbands (DD1, DD2) each have a mean transmittance of at least 0.5, typically at least 0.8 or at least 0.9; wherein 350 nm?D?1<D?2<D?3<D?4<D?5<D?6<D?7<1000 nm.

Abstract: An optical coherence tomograph that provides wavelength tunable source radiation and an illumination and measurement beam path, a dividing element that divides source radiation into illumination radiation and reference radiation, and collects measurement radiation. The illumination and measurement beam path has scanner. A detection beam path receives measurement radiation and reference radiation and conducts them onto at least one flat panel detector in a superposed manner. A beam splitter separates the measurement radiation from the illumination radiation. The beam splitter conducts the separated measurement radiation to the detection beam path and sets the numerical aperture of the illumination of the illumination field in the eye. An optical element sets the numerical aperture with which the measurement radiation is collected in the eye and a multi-perforated aperture defines the size of an object field and a number of object spots, from which the measurement radiation reaches the flat panel detector.

Abstract: In a microscope for high resolution scanning microscopy of a sample, said microscope comprising—an illumination device for illuminating the sample, —an imaging device for scanning at least one point spot or line spot across the sample and for imaging the point spot or line spot into a diffraction-limited, stationary single image with magnification into a detection plane, —a detector device for detecting the single image in the detection plane for different scanning positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited single image, —an evaluation device for evaluating a diffraction pattern of the single image for the scanning positions from data of the detector device and for generating an image of the sample, said image having a resolution that is increased beyond the diffraction limit, provision is made for—the detector device to have a detector array, which has pixels and is larger than t

Abstract: Each modulator of an image projector includes multiple pixels arranged in rows and columns, switchable independently of each other into a first state, in which light incident on them is used to generate an image, and into a second state, in which light incident on them is not used to generate an image. At least one first pixel is assigned to each second pixel such that the imaging optical system a) strikes the second pixel with light from the respective assigned first pixel when the assigned first pixel is switched into the first state, in order to illuminate the second pixel actively, and b) does not strike the second pixel it with light from the respective assigned first pixel when the assigned first pixel is switched into the second state, in order not to illuminate the second pixel actively. A control unit that controls the modulators is also disclosed.

Abstract: Each modulator of an image projector includes multiple pixels arranged in rows and columns, switchable independently of each other into a first state, in which light incident on them is used to generate an image, and into a second state, in which light incident on them is not used to generate an image. At least one first pixel is assigned to each second pixel such that the imaging optical system a) strikes the second pixel with light from the respective assigned first pixel when the assigned first pixel is switched into the first state, in order to illuminate the second pixel actively, and b) does not strike the second pixel it with light from the respective assigned first pixel when the assigned first pixel is switched into the second state, in order not to illuminate the second pixel actively. A control unit that controls the modulators is also disclosed.

Abstract: In a microscope for high resolution scanning microscopy of a sample, said microscope comprising—an illumination device for illuminating the sample, —an imaging device for scanning at least one point spot or line spot across the sample and for imaging the point spot or line spot into a diffraction-limited, stationary single image with magnification into a detection plane, —a detector device for detecting the single image in the detection plane for different scanning positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited single image, —an evaluation device for evaluating a diffraction pattern of the single image for the scanning positions from data of the detector device and for generating an image of the sample, said image having a resolution that is increased beyond the diffraction limit, provision is made for—the detector device to have a detector array, which has pixels and is larger than t

Abstract: A light microscope having a specimen plane, in which a specimen to be examined is positioned, having a light source to emit illuminating light, having optical imaging means to convey the illuminating light into the specimen plane, having a first scanning means, with which an optical path of the illuminating light and the specimen can be moved relative to each other to produce an illumination scanning movement of the illuminating light relative to the specimen, having a detector means to detect specimen light coming from the specimen and having electronic means to produce an image of the specimen based on the specimen light detected by the detector means at different specimen regions. A second scanning means is present, with which it can be adjusted which specimen region can be imaged on a determined detector element.

Abstract: In a microscope for high resolution scanning microscopy of a sample, said microscope comprising—an illumination device for illuminating the sample, —an imaging device for scanning at least one point spot or line spot across the sample and for imaging the point spot or line spot into a diffraction-limited, stationary single image with magnification into a detection plane, —a detector device for detecting the single image in the detection plane for different scanning positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited single image, —an evaluation device for evaluating a diffraction pattern of the single image for the scanning positions from data of the detector device and for generating an image of the sample, said image having a resolution that is increased beyond the diffraction limit, provision is made for—the detector device to have a detector array, which has pixels and is larger than t

Abstract: A multifocal representation device with a digital image generation module and a control unit, which conveys to the digital image generation module two-dimensional image data of a three-dimensional object to be represented, is provided, wherein the digital image generation module, based on the two-dimensional image data conveyed, generates two-dimensional images of the object from at least two different object planes in corresponding different focal planes in such a way that an observer can focus with his eye on the different focal planes in order to perceive the represented object three-dimensionally, and wherein the control unit determines for each two-dimensional image to be represented a sharpness value in sections and sets the image data to dark for the image sections the sharpness value of which lies outside a sharpness value range predetermined for the image.

Abstract: A method for operating a scanning microscope and for determining point spread functions, with which sample images are recorded with the scanning microscope. The method includes scanning a sample with at least one illuminating light beam; recording at least one sample image with a detector device during a scan by the illuminating light beam; and comprising the point spread function, with which a sample image is recorded, from the at least one sample image. A detector device having receiving elements is used, where the distance between the receiving elements is smaller than a diffraction disk that generates a sample point on the detector device. Detector signals, generated by means of the receiving elements, are read out for each of the different positions of the illuminating light beam on the sample, as a result of which the scanning of the sample allows the detector signals, which are read out, to generate a plurality of sample images.

Abstract: A method and device are provided for time-sequential recording of three-dimensional images each of which has at least one first and one second partial image. The device has a sensor (13) with pixels (16) subdivided into two mutually different pixel groups. An imaging optical unit has a switchable changeover device (9) that images the partial images of the three-dimensional image time-sequentially onto the sensor (13). A control unit is connected to the sensor (13) and the changeover device (9) to control the reading of the sensor (13) and the switching states of the changeover device (9) so that the changeover device (9), during the imaging of the partial images onto the sensor (13) assumes at least one switching state in which excerpts of different partial images are fed to the different pixel groups of the sensor (13).

Abstract: A microscope and method for high resolution scanning microscopy of a sample, having an illumination device, an imaging device for the purpose of scanning at least one point or linear spot across the sample and of imaging the point or linear spot into a diffraction-limited, static single image below a reproduction scale in a detection plane. A detector device is used for detecting the single image in the detection plane for various scan positions, with a location accuracy which, taking into account the reproduction scale in at least one dimension/measurement, is at least twice as high as a full width at half maximum of the diffraction-limited single image.

Abstract: A projection device includes a first and a second tilting mirror matrix, each including a plurality of tilting mirrors. A cover glass covers the tilting mirrors. An imaging lens system includes a relay lens system which images the tilting mirrors of the first tilting mirror matrix onto the tilting mirrors of the second tilting mirror matrix. Thus light reflected by the tilting mirrors of the first tilting mirror matrix onto tilting mirrors of the second tilting mirror matrix, and a projection lens system, which projects light reflected by tilting mirrors of the second tilting mirror matrix, in order to produce an image, is provided. The imaging lens system further includes a correction element which corrects at least one image error caused by the light obliquely passing through the cover glasses.

Abstract: Provided is an imaging method in which a surface of resected matter is imaged in first images with a photoacoustic image generation method down to a first predetermined depth such that in the first images there is a contrast between pathological tissue, in particular cancerous tissue, and non-pathological tissue.

Abstract: A projection device includes a first and a second tilting mirror matrix, each including a plurality of tilting mirrors. A cover glass covers the tilting mirrors. An imaging lens system includes a relay lens system which images the tilting mirrors of the first tilting mirror matrix onto the tilting mirrors of the second tilting mirror matrix. Thus light reflected by the tilting mirrors of the first tilting mirror matrix onto tilting mirrors of the second tilting mirror matrix, and a projection lens system, which projects light reflected by tilting mirrors of the second tilting mirror matrix, in order to produce an image, is provided. The imaging lens system further includes a correction element which corrects at least one image error caused by the light obliquely passing through the cover glasses.

Abstract: A projection device includes a first and a second tilting mirror matrix, each including a plurality of tilting mirrors. A cover glass covers the tilting mirrors. An imaging lens system includes a relay lens system which images the tilting mirrors of the first tilting mirror matrix onto the tilting mirrors of the second tilting mirror matrix. Thus light reflected by the tilting mirrors of the first tilting mirror matrix onto tilting mirrors of the second tilting mirror matrix, and a projection lens system, which projects light reflected by tilting mirrors of the second tilting mirror matrix, in order to produce an image, is provided. The imaging lens system further includes a correction element which corrects at least one image error caused by the light obliquely passing through the cover glasses.

Abstract: A multifocal representation device with a digital image generation module and a control unit, which conveys to the digital image generation module two-dimensional image data of a three-dimensional object to be represented, is provided, wherein the digital image generation module, based on the two-dimensional image data conveyed, generates two-dimensional images of the object from at least two different object planes in corresponding different focal planes in such a way that an observer can focus with his eye on the different focal planes in order to perceive the represented object three-dimensionally, and wherein the control unit determines for each two-dimensional image to be represented a sharpness value in sections and sets the image data to dark for the image sections the sharpness value of which lies outside a sharpness value range predetermined for the image.

Abstract: Microscope and method for high resolution scanning microscopy of a sample, wherein the sample is illuminated; at least one point spot or line spot, which is guided in a scanning manner over the sample, is imaged into a still image; wherein the spot is imaged in a diffraction limited manner into the still image with magnification, and the still image lies still in a plane of detection; the still image is detected for different scan positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited still image, so that a diffraction pattern of the still image is detected; the diffraction pattern of the still image is evaluated for each scan position, and an image of the sample is generated that has a resolution that is increased beyond the diffraction limit, wherein a detector array is provided that has pixels and is larger than the still image; and radiation of the still image from the plane of detecti

Abstract: In a microscope for high resolution scanning microscopy of a sample, said microscope comprising—an illumination device for illuminating the sample,—an imaging device for scanning at least one point spot or line spot across the sample and for imaging the point spot or line spot into a diffraction-limited, stationary single image with magnification into a detection plane ,—a detector device for detecting the single image in the detection plane for different scanning positions with a spatial resolution, which, taking into consideration the magnification, is at least twice as high as a full width at half maximum of the diffraction-limited single image,—an evaluation device for evaluating a diffraction pattern of the single image for the scanning positions from data of the detector device and for generating an image of the sample, said image having a resolution that is increased beyond the diffraction limit, provision is made for—the detector device to have a detector array, which has pixels and is larger than the