Abstract: Fluorescent probe compounds comprising fluorescent cholic acid derivative are used for visualizing the influence of a candidate compound on biliary excretion in in vitro or in vivo biological models, including certain probes developed report on the activity of the bile salt export pump (BSEP). Visualization is done on hepatocyte cultures with formed bile canaliculi or on liver systems that are exposed to the fluorescent probes and the candidate compound. Visualization is done by fluorescence microscopy. The probes are particularly suitable for early screening of multiple candidate compounds.

Abstract: Methods and processes for cryopreservation and direct cell thawing and seeding or suspension after cryopreservation, including methods that eliminate the necessity of post-thaw wash, spin, and frequent practice of performing a cell count. Cell compositions and no-spin cell products produced using the methods are also described.

Abstract: Methods and processes for cryopreservation and direct cell thawing and seeding or suspension after cryopreservation, including methods that eliminate the necessity of post-thaw wash, spin, and frequent practice of performing a cell count. Cell compositions and no-spin cell products produced using the methods are also described.

Abstract: Method for packaging cells in culture in a low-pressure environment, and corresponding device. The invention relates to a method for packaging live cells in culture for the purpose of transporting them, wherein said cells are in a culture medium contained in at least one container, characterized in that said method comprises: a phase of filling said container(s) according to a given degree of filling; a step of closure of said container(s) comprising: bagging said container(s) in a flexible sachet; and reducing the pressure inside said sachet; sealing said sachet, with the pressure reduction being maintained, wherein said closure step is carried out under temperature conditions correlated with said degree of filling with a view to avoiding the boiling of said culture medium during said pressure reduction.

Abstract: Method for packaging cells in culture in a low-pressure environment, and corresponding device. The invention relates to a method for packaging live cells in culture for the purpose of transporting them, wherein said cells are in a culture medium contained in at least one container, characterized in that said method comprises: a phase of filling said container(s) according to a given degree of filling; a step of closure of said container(s) comprising: bagging said container(s) in a flexible sachet; and reducing the pressure inside said sachet; sealing said sachet, with the pressure reduction being maintained, wherein said closure step is carried out under temperature conditions correlated with said degree of filling with a view to avoiding the boiling of said culture medium during said pressure reduction.