Abstract: The present invention relates to a method of screening the operating conditions of a coupling reaction of at least two functional groups, and in particular of catalysts that are useful in this coupling reaction, and also to kits suitable for allowing this method to be carried out. Applications: fundamental and applied research, in particular in the areas of chemistry, agrofoods, pharmacy and environmental protection, for the development or optimization of synthesis reaction yields.

Abstract: This detection method uses a solid support whereon plasminogen is immobilized, and includes essentially the following steps: (a) preparing the biological sample during which the latter is incubated on a homogenizing buffer which comprises an ionic or nonionic surfactant, a glucose-containing buffer, a saccharose-based buffer and a PBS buffer or in a capture buffer comprising an ionic surfactant; said step optionally comprising incubation with a proteinase K at a final concentration ranging between 1 and 8 ?g/ml; (b) capturing the PrPres on the solid support, which is carried out in the presence of a capture buffer, as defined above; (c) a controlled denaturation of the PrPres fixed on the support comprising incubation with a denaturation buffer including at least one chaotropic agent, at a temperature ranging between room temperature and 100° C.; and (d) detecting the denatured PrPres fixed on said support with a PrP protein-specific antibody.

Abstract: The invention concerns a method for heterogeneous phase detection of an analyte, the trifunctional reagent used for implementing said method, its use for detecting an analyte, as well as the device for detecting a corresponding analyte