Abstract: A reader card for use in densitometric test analysis wherein a film having data recorded as spots thereon is supported by and retained on a base card, the reader card further having an area for use in establishing initial conditions for the optical system of the densitometer and a series of borders for indicating the relative position of the film on the card to the optical system of the densitometer. In a preferred embodiment, the reader card includes a base card having an opening therethrough, and an overlay thereover. The overlay retains the film in place and includes a black band covering the film, the black band having windows therethrough for observing and measuring the spots.