Abstract: In an illustrative embodiment, the present disclosure provides methods, systems and/or instruments for the automated editing of immune cells for chimeric antigen receptor therapies.

Abstract: An improved, preferably oil-less, air compressor assembly includes a frame and at least a first rotatable cam member mounted to the frame for selective rotation relative thereto. The first cam member has a cam profile. A motor is operatively interconnected with the first cam member for rotating the first cam member. A dual-acting piston assembly includes first and second rods operatively interconnected with a piston received in a piston housing, wherein the piston assembly is mounted relative to the cam profile whereby the piston is stroked upon rotation of the first cam member.

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.

Abstract: A patron tracking system for tracking patron activity in a casino enterprise, wherein the casino enterprise comprises a home casino and one or more non-home casinos, the patron tracking system comprising: a home local casino management system at the home casino; one or more non-home casino management systems at the one or more non-home casinos; wherein the local casino management systems contain customer data related to patron activity; a message router for transmitting customer data between the home and non-home local casino management systems; and wherein certain customer data residing on the home local casino management system is not shared with the one or more non-home local casino management systems.