Abstract: A video control, projection and display system is employed in a gaming establishment and used in conjunction with or without a gaming network or gaming components. The control system includes projection cameras or light sources which may generate and display images associated with the electronic gaming machines or with a player of the electronic gaming machines. The control system enhances the image by using pixel cloning, image blending pixel scaling, pixel movement, and pixel masking. The projection system may generate holographic images and may project images onto a variety of surfaces adjacent to the electronic gaming machines. The control system manages devices associated with the projection and display system.

Abstract: The present disclosure pertains to compositions, methods, and kits for improving the rate of double-strand break (DSB) repair by homology directed repair (HDR) through inhibition of 53BP1 mediated DNA end protection in combination with inhibition of microhomology-mediated end joining (MMEJ), or alternatively in combination with inhibition of non-homologous end joining (NHEJ), or in combination with inhibition of both MMEJ and NHEJ.

Abstract: A memory access request is initiated by a first peripheral device. The memory access request includes a virtual address. A host physical address (HPA) associated with the virtual address is determined. An identifier of a base address register (BAR) associated with the HPA is determined by the first peripheral device. The memory access request is transmitted using the identifier of the BAR to a second peripheral device.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: A method and system for dispensing metered shots of cannabis-containing or hemp-containing concentrate into a receiving vessel. The method comprises the steps of (a) providing a control module that includes a touch screen; (b) communicating the control module with an endless piston pump or progressive cavity pump; (c) deploying a disposable plastic tube and plunger to dispense bulk concentrate into the pump; (d) positioning an empty receiving vessel into a position in relation to the dispenser using a conveyor, or a turntable, or by manually placing the receiving vessel into position; (e) dispensing a metered shot of the concentrate into the empty receiving vessel to prepare a filled vessel; and (f) re-positioning the filled vessel in registration with the conveyor or turntable so that the dispenser becomes engaged with a subsequent empty receiving vessel.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: Typically, fluids used in fracking operations erode valve seats in the fluid end of the reciprocating pump. Thus, these valve seats must frequently be replaced by new valve seats. Accordingly, a remanufacturing process is disclosed that successively cleans and/or grinds manually or mechanically at least the carbide inserts from used valve seats until the inserts are reusable. This reduces material costs and promotes efficiency and sustainability.

Abstract: This invention pertains to mutant Lachnospiraceae bacterium ND2006 (Lb) Cas12a nucleic acids and proteins for use in CRISPR/Cas12a endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant LbCas12a protein, wherein the isolated mutant LbCas12a protein is active in a CRISPR/Cas12a endonuclease system. The invention also includes isolated nucleic acids encoding mutant LbCas12a proteins, ribonucleoprotein complexes and CRISPR/Cas12a endonuclease systems having mutant LbCas12a proteins.

Abstract: This disclosure provides a method for installing a basketball goal by using an actuator. The actuator, in a non-inverted orientation, is used to raise and lower a backboard for the basketball goal. The actuator can be inverted and used as a crank to raise a hinged post of the goal into an upright orientation.

Abstract: In some examples, a computer receives a command to transport a storage cartridge in a storage system from a physical storage slot to a storage drive, and determines whether the storage cartridge is associated with a restricted transport indication set by a configuration operation in the storage system. In response to determining that the storage cartridge is associated with the restricted transport indication, the computer checks for an indication of receipt of an access secret information, and denies processing of the command to transport the storage cartridge if the indication of receipt of the access secret information is not present.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRISPR/Cas endonuclease systems having mutant Cas9 proteins that display increased on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: This invention pertains to optimized protein fusion linkers for creating multi-functional chimeric proteins and methods of using the same. Additionally, the invention pertains to chimeric proteins for use in guided endonuclease systems and methods of using the same.

Abstract: A method and system for dispensing metered shots of cannabis-containing or hemp-containing concentrate into a receiving vessel. The method comprises the steps of (a) providing a control module that includes a touch screen; (b) communicating the control module with an endless piston pump or progressive cavity pump; (c) deploying a disposable plastic tube and plunger to dispense bulk concentrate into the pump; (d) positioning an empty receiving vessel into a position in relation to the dispenser using a conveyor, or a turntable, or by manually placing the receiving vessel into position; (e) dispensing a metered shot of the concentrate into the empty receiving vessel to prepare a filled vessel; and (f) re-positioning the filled vessel in registration with the conveyor or turntable so that the dispenser becomes engaged with a subsequent empty receiving vessel.

Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.

Abstract: This invention pertains to mutant Lachnospiraceae bacterium ND2006 (Lb) Cas12a nucleic acids and proteins for use in CRISPR/Cas12a endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant LbCas12a protein, wherein the isolated mutant LbCas12a protein is active in a CRISPR/Cas12a endonuclease system. The invention also includes isolated nucleic acids encoding mutant LbCas12a proteins, ribonucleoprotein complexes and CRISPR/Cas12a endonuclease systems having mutant LbCas12a proteins.

Abstract: Typically, fluids used in fracking operations erode valve seats in the fluid end of the reciprocating pump. Thus, these valve seats must frequently be replaced by new valve seats. Accordingly, a remanufacturing process is disclosed that successively cleans and/or grinds manually or mechanically at least the carbide inserts from used valve seats until the inserts are reusable. This reduces material costs and promotes efficiency and sustainability.

Abstract: A process includes communicating by a first device, with a second device. The communicating includes the first device receiving data from the second device that represents a certificate. The certificate binds a hierarchy of logical identifiers to a cryptographic key. The hierarchy of identifiers includes a first logical identifier that corresponds to a group membership. The process includes authenticating, by the first device, the second device based on the certificate. The process includes allowing, by the first device, a secure connection to be set up between the first device and the second device based on whether the first logical identifier represents that the second device is a member of a first group of devices of which the first device is a member.

Abstract: Methods, systems, and apparatus for a system that communicates with other vehicles using light signals. The system includes one or more light emitters coupled to a vehicle and an electronic control unit coupled to the light emitter(s). The electronic control unit is configured to encode a message using a communication protocol to create a first light pattern. The electronic control unit is further configured to generate a light pattern using the light emitter(s) to communicate the message to an adjacent vehicle. The message indicates vehicle, passenger, and/or roadway conditions to provide the adjacent vehicle real-time conditions, which can be used to provide immediate feedback that the adjacent vehicle can use to safely control one or more aspects of the adjacent vehicle.

Abstract: Typically, fluids used in fracking operations erode valve seats in the fluid end of the reciprocating pump. Thus, these valve seats must frequently be replaced by new valve seats. Accordingly, a remanufacturing process is disclosed that successively cleans and/or grinds manually or mechanically at least the carbide inserts from used valve seats until the inserts are reusable. This reduces material costs and promotes efficiency and sustainability.

Abstract: A method of determining performance of GNSS prediction data includes receiving the GNSS prediction data at a server, the GNSS prediction data being associated with a unique identifier, determining performance metrics of the prediction data at a current time and saving the performance metrics of the prediction data in association with the unique identifier, and in response to receipt of a request from an electronic device, sending one or both of the performance metrics of the prediction data associated with the unique identifier and corrections for correcting the prediction data to the electronic device for use by the electronic device.