Abstract: A disposable sensor cap (100) configured to attach to a cell culture device comprises a plurality of sensors for measuring cell culture media conditions. For example, the plurality of sensors (180) may measure dissolved gases and essential molecules of choice without the risk of contamination of the vessel. The sensors may be integrated in a body (150) of the sensor cap. During the measurement period, a small volume of cell culture media may be captured in a sampling chamber of the sensor cap when the cell culture vessel is tipped. An inductor coil (160) on the sensor cap allows for noninvasive wireless reading of the measurements.

Abstract: Ultrasonic devices comprise a housing comprising an ultrasonic surface, an ultrasonic transducer, and an ultrasonic horn to provide and focus energy from the ultrasonic transducer to the ultrasonic surface. Methods of removing trapped air bubbles from a cell culture container comprise positioning a cell culture container comprising air bubbles trapped in liquid within microcavity wells of the cell culture container at an ultrasonic surface of an ultrasonic device. Mechanical agitation is generated by the ultrasonic device and applied to the cell culture container to remove the trapped air bubbles from the microcavity wells. Methods of releasing cell aggregates from a cell culture container comprising positioning a cell culture container comprising cell aggregates in microcavity wells at an ultrasonic surface of an ultrasonic device. The cell aggregates are released from the microcavity wells by applying mechanical agitation from the ultrasonic device to a surface of the cell culture container.