Abstract: Provided herein are methods for discovery of epitope specific monoclonal antibodies to pathogens directly from immune sera for immunotherapeutic use. Further provided herein are methods to determine molecular structure of antibodies targeting an antigen from convalescent or vaccinated individuals for the purpose of rational vaccine design.

Abstract: Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: The invention relates to germline-targeting designs, stabilization designs, and/or combinations thereof, of proteins designed with modified surfaces helpful for immunization regimens, other protein modifications and/or development of nanoparticles, methods of making and using the same, and to (a) germline-targeting priming or boosting/shepherding immunogens to initiate or guide maturation of VRC01-class responses (b) PCT64/PG9-germline-targeting designs (c) BG18-germline-targeting designs or boosting/shepherding immunogens to initiate or guide maturation of BG18-like responses, and/or (d) trimer stabilization and presentation in a membrane-bound format.

Abstract: Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Abstract: Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Abstract: Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

Abstract: A first party, such as a residential subscriber to an Internet Service Provider (ISP), logically partitions its computing resources into an end user partition and a crowd sourced cloud partition. The first party installs a crowd sourced cloud application in each cloud partition. Together, a cloud provider computing system and each cloud application orchestrate cloud services over a communications network, such as the Internet. For each crowd sourced cloud application, orchestration involves registering cloud services with the cloud provider, provisioning each registered cloud service that is requested by the cloud provider, and operating each provisioned service. For the cloud provider, orchestration involves publishing each registered service as available to crowd sourced cloud users, receiving requests for cloud services from a crowd sourced cloud user, and requesting, from a crowd sourced cloud application, a registered service responsive to the request for cloud services.

Abstract: Methods and apparatus for managing interruptions in a multiple communication mode environment are provided herein. For example, a method may include receiving at least first instance of communication data associated with a first communication mode; obtaining first attribute data related to the first instance of communication data; classifying the first instance of communication data into first category based on the first attribute data using the interruption management device; and determining whether to interrupt a user by delivering the first instance of communication data based on the first category. The first category may be selected from a plurality of predetermined categories using a classification algorithm.

Abstract: A first party, such as a residential subscriber to an Internet Service Provider (ISP), logically partitions its computing resources into an end user partition and a crowd sourced cloud partition. The first party installs a crowd sourced cloud application in each cloud partition. Together, a cloud provider computing system and each cloud application orchestrate cloud services over a communications network, such as the Internet. For each crowd sourced cloud application, orchestration involves registering cloud services with the cloud provider, provisioning each registered cloud service that is requested by the cloud provider, and operating each provisioned service. For the cloud provider, orchestration involves publishing each registered service as available to crowd sourced cloud users, receiving requests for cloud services from a crowd sourced cloud user, and requesting, from a crowd sourced cloud application, a registered service responsive to the request for cloud services.

Abstract: Methods and apparatus for managing interruptions in a multiple communication mode environment are provided herein. For example, a method may include receiving at least first instance of communication data associated with a first communication mode; obtaining first attribute data related to the first instance of communication data; classifying the first instance of communication data into first category based on the first attribute data using the interruption management device; and determining whether to interrupt a user by delivering the first instance of communication data based on the first category. The first category may be selected from a plurality of predetermined categories using a classification algorithm.

Abstract: Methods and apparatus for managing interruptions in a multiple communication mode environment are provided herein. For example, a method may include receiving at least first and second instances of communication data associated with first and second communication modes; obtaining first and attribute data related to the first and second instances of communication data; classifying the first and second instances of communication data into first and second categories based on the first and second attribute data using the interruption management device; and determining whether to interrupt a user by delivering the first instance of communication data or the second instance of communication data based on the first and second categories, respectively. The first category and the second category may be selected from a plurality of predetermined categories using a classification algorithm. The first communication mode may be different than the second communication mode.

Abstract: Methods and apparatus for managing interruptions in a multiple communication mode environment are provided herein. For example, a method may include receiving at least first and second instances of communication data associated with first and second communication modes; obtaining first and attribute data related to the first and second instances of communication data; classifying the first and second instances of communication data into first and second categories based on the first and second attribute data using the interruption management device; and determining whether to interrupt a user by delivering the first instance of communication data or the second instance of communication data based on the first and second categories, respectively. The first category and the second category may be selected from a plurality of predetermined categories using a classification algorithm. The first communication mode may be different than the second communication mode.