Abstract: P63 and p73 (and their genes TP53, TP63, TP73) are truncated at or around the tetramerization domain and fused in frame to a heterologous tetramerization domain from a bacterial gene/protein or viral gene/protein or non-human protein lacking homology to any human proteins. Alternatively, the native tetramerization domain can be excised leaving some 3? sequences. These fusion proteins have the activity of the original protein but are unable to hetero-tetramerize or dimerize or be negatively regulated. Further, since non-human tetramerization sequences with minimal homology to human sequences are used, the fusion proteins will not interact with other tetramerization domains in the cell. Gene therapy treatments can thus be used to treat diseases in which these proteins are implicated, such as cancer, autoimmune, and autoinflammatory diseases.

Abstract: MiniVectors and compositions containing MiniVectors that target ovarian cancer genes selected from FOXM1, AKT, CENPA, PLK1, CDC20, BIRC5, AURKB, CCNB1, CDKN3, BCAM-AKT2, CDKN2D-WDFY2, SLC25A6, CIP2A, CD133, ALDH1A1, CD44, SALL4, and/or PRDM16, alone or in any combination, are provided, along with uses in the treatment of ovarian cancer.