Patents by Inventor Christopher H. Taron
Christopher H. Taron has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230416701Abstract: The present disclosure relates, according to some embodiments, to compositions, methods, and/or kits for producing vaccinia capping enzyme. For example, active, heterodimers of vaccinia capping enzyme may be produced as fusions comprising D1 and D12 subunits. Vaccinia capping enzyme fusion proteins may further comprise a linker.Type: ApplicationFiled: September 7, 2023Publication date: December 28, 2023Applicant: New England Biolabs, Inc.Inventors: Saulius Vainauskas, Siu-hong Chan, Christopher H. Taron
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Publication number: 20230416705Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.Type: ApplicationFiled: June 23, 2023Publication date: December 28, 2023Applicant: New England Biolabs, Inc.Inventors: Mehul Ganatra, Siu-hong Chan, Christopher H. Taron, G. Brett Robb
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Patent number: 11788074Abstract: The present disclosure relates, according to some embodiments, to compositions, methods, and/or kits for producing vaccinia capping enzyme. For example, active, heterodimers of vaccinia capping enzyme may be produced as fusions comprising D1 and D12 subunits. Vaccinia capping enzyme fusion proteins may further comprise a linker.Type: GrantFiled: June 15, 2021Date of Patent: October 17, 2023Assignee: New England Biolabs, Inc.Inventors: Saulius Vainauskas, Siu-Hong Chan, Christopher H. Taron
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Patent number: 11725196Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.Type: GrantFiled: July 16, 2021Date of Patent: August 15, 2023Assignee: New England Biolabs, Inc.Inventors: Mehul Ganatra, Siu-hong Chan, Christopher H. Taron, G. B. Robb
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Publication number: 20220235339Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.Type: ApplicationFiled: July 16, 2021Publication date: July 28, 2022Applicant: New England Biolabs, Inc.Inventors: Mehul Ganatra, Siu-hong Chan, Christopher H. Taron, G. B. Robb
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Publication number: 20210395706Abstract: The present disclosure relates, according to some embodiments, to compositions, methods, and/or kits for producing vaccinia capping enzyme. For example, active, heterodimers of vaccinia capping enzyme may be produced as fusions comprising D1 and D12 subunits. Vaccinia capping enzyme fusion proteins may further comprise a linker.Type: ApplicationFiled: June 15, 2021Publication date: December 23, 2021Applicant: New England Biolabs, Inc.Inventors: Saulius Vainauskas, Siu-hong Chan, Christopher H. Taron
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Patent number: 11098295Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.Type: GrantFiled: April 30, 2021Date of Patent: August 24, 2021Assignee: New England Biolabs, Inc.Inventors: Mehul Ganatra, Siu-Hong Chan, Christopher H. Taron, G. B. Robb
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Patent number: 11028379Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.Type: GrantFiled: January 27, 2021Date of Patent: June 8, 2021Assignee: New England Biolabs, Inc.Inventors: Mehul Ganatra, Siu-hong Chan, Christopher H. Taron, G. B. Robb
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Patent number: 10507233Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.Type: GrantFiled: August 20, 2018Date of Patent: December 17, 2019Assignee: New England Biolabs, Inc.Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
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Patent number: 10260056Abstract: Provided herein is an ?-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The ?-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the ?-fucosidase are provided, as well as a method for analyzing glycoproteins. The ?-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.Type: GrantFiled: February 12, 2018Date of Patent: April 16, 2019Assignee: New England Biolabs, Inc.Inventors: Christopher H. Taron, Saulius Vainauskas, Xiaofeng Shi
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Publication number: 20190008934Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomoleules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.Type: ApplicationFiled: August 20, 2018Publication date: January 10, 2019Applicant: New England Biolabs, Inc.Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
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Patent number: 10080787Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.Type: GrantFiled: June 26, 2015Date of Patent: September 25, 2018Assignee: New England Biolabs, Inc.Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
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Publication number: 20180265910Abstract: Provided herein is an ?-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The ?-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with BKF. A reaction mix, enzyme mix and kit comprising the ?-fucosidase are provided, as well as a method for analyzing glycoproteins. The ?-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.Type: ApplicationFiled: March 17, 2017Publication date: September 20, 2018Applicant: New England Biolabs, Inc.Inventors: Christopher H. Taron, Saulius Vainauskas, Xiaofeng Shi
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Publication number: 20180265854Abstract: Provided herein is an ?-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The ?-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with bovine kidney fucosidase. A reaction mix, enzyme mix and kit comprising the ?-fucosidase are provided, as well as a method for analyzing glycoproteins. The ?-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.Type: ApplicationFiled: February 12, 2018Publication date: September 20, 2018Applicant: New England Biolabs, Inc.Inventors: Christopher H. Taron, Saulius Vainauskas, Xiaofeng Shi
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Patent number: 9964548Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: GrantFiled: December 28, 2016Date of Patent: May 8, 2018Assignee: New England Biolabs, Inc.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Publication number: 20170138959Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: ApplicationFiled: December 28, 2016Publication date: May 18, 2017Applicant: New England Biolabs, Inc.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Publication number: 20170128554Abstract: Methods of capturing N-glycan linked glycomolecules including N-glycans, N-glycopeptides and N-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged N-glycans and/or N-glycan linked glycomolecules. Binding reagents for substantially unbiased binding of N-glycans and/or N-glycan linked glycomolecules are also described.Type: ApplicationFiled: June 26, 2015Publication date: May 11, 2017Applicant: New England Biolabs, Inc.Inventors: Minyong Chen, Xiaofeng Shi, James C. Samuelson, Christopher H. Taron
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Publication number: 20150346194Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: ApplicationFiled: May 29, 2015Publication date: December 3, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Patent number: 8647855Abstract: Compositions and methods are provided for generating biofuels by fermentation from carbon sources other than glucose using genetically engineered yeast strains. For example, a Saccharomyces strain which is capable of converting glucose to ethanol but not of metabolizing N-acetyl glucosamine is genetically engineered to utilize N-acetyl glucosamine as a nutrient carbon source.Type: GrantFiled: November 16, 2009Date of Patent: February 11, 2014Assignee: New England Biolabs, Inc.Inventors: Christopher H. Taron, Paul A. Colussi
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Patent number: 8623615Abstract: Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility. The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consists of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.Type: GrantFiled: April 1, 2009Date of Patent: January 7, 2014Assignee: New England Biolabs, Inc.Inventors: Paul Riggs, Iris Walker, Paul A. Colussi, Mehul Ganatra, Christopher H. Taron