Publication number: 20210130892
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Application
Filed:
October 29, 2020
Publication date:
May 6, 2021
Inventors:
Phillip Belgrader, Zachary Bent, Rajiv Bharadwaj, Vijay Kumar Sreenivasa Gopalan, Josephine Harada, Christopher Hindson, Mohammad Rahimi Lenji, Michael Ybarra Lucero, Geoffrey McDermott, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Tobias Daniel Wheeler, Indira Wu, Solongo Batjargal Ziraldo, Stephane Claude Boutet, Sarah Taylor, Niranjan Srinivas
Publication number: 20210102936
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).
Type:
Application
Filed:
June 9, 2020
Publication date:
April 8, 2021
Inventors:
Zahra Kamila Belhocine, Jason Bell, Zachary Bent, Rajiv Bharadwaj, Christopher Hindson, Mohammad Rahimi Lenji, Bill Lin, Anthony Makarewicz, Geoffrey McDermott, Elliott Meer, Francesca Meschi, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Michael Schnall-Levin, Sarah Taylor, Jessica Michele Terry, Tobias Daniel Wheeler, Yifeng Yin, Xinying Zheng, Solongo Batjargal Ziraldo, Eswar Prasad Ramachandran Iyer, Luigi Jhon Alvarado Martinez
Patent number: 10793905
Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.
Type:
Grant
Filed:
November 22, 2019
Date of Patent:
October 6, 2020
Assignee:
10X GENOMICS, INC.
Inventors:
Zachary Bent, Josephine Harada, Christopher Hindson, Elliott Meer, Tarjei Sigurd Mikkelsen, Christopher Joachim O'Keeffe, Katherine Pfeiffer, Andrew D. Price, Paul Ryvkin, Serge Saxonov, John R. Stuelpnagel, Jessica Michele Terry, Solongo Batjargal Ziraldo