Abstract: The present disclosure relates to a humanized and CMC properties-improved anti-DKK2 antibody, a composition containing the antibody, and a method for treating diseases using the antibody or the composition.

Abstract: Systems and methods are provided for use in applying treatments to crops in fields. One example computer-implemented method includes calculating a growth stage of a crop in a field on a defined date based on planting data and weather data for the field and/or crop, and then, in response to the growth stage being within a spray window for the crop, defining a plurality of synthetic sprays within the spray window for the field. The method then includes, for each one of the synthetic sprays, calculating at least one disease risk for the crop in the field and calculating a response to the synthetic spray. The method then further includes compiling a report including a selected one or more of the responses, based on yield differences of the responses, as a recommendation for applying the treatment to the crop consistent with the synthetic spray associated with the selected one or more of the responses.

Abstract: Incoherently combining light from different lasers while maintaining high brightness is challenging using conventional fiber bundling techniques, where fibers from different lasers are bundled adjacently in a tight-packed arrangement. The brightness can be increased by tapering the tips of the bundled fibers to match a single, multi-mode output fiber, e.g., one whose core that is just wide enough to fit the input cores. This increases the brightness of the beam combining. In addition, reducing the outer diameters of the signal fiber claddings allows the signal fibers to be bundled closer together, making it possible to couple more signal fiber cores to the core of a multi-mode output fiber. Similarly, reducing the outer diameter of the pump fiber cladding and/or etching away corresponding portions of the signal fiber cladding in a pump/signal combiner makes it possible to couple more pump light into the signal fiber cladding, again increasing brightness.

Abstract: Arrays of fiber pigtails can be used to project and receive light. Unfortunately, most fiber pigtail arrays are not aligned well enough for coherently combining different optical beams. This imprecision stems in part from misalignment between the optical fiber and the endcap spliced to the end of the optical fiber. The endcap is often polished, curved, or patterned, causing the light emitted by the endcapped fiber to refract or diffract as it exits the endcap. This refraction or diffraction shifts the apparent position of the beam waist from its actual position. Measuring this virtual beam waist position before and after splicing the endcap to the fiber increases the absolute precision with which the fiber is aligned to the endcap. This increase in absolute precision reduces the deviation in virtual beam waist position among endcapped fibers, making it easier to produce arrays of endcapped fibers aligned precisely enough for coherent beam combining.

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract: Provided herein are recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human ?-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A protein).

Abstract: Provided herein are recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human ?-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A protein).

Abstract: Incoherently combining light from different lasers while maintaining high brightness is challenging using conventional fiber bundling techniques, where fibers from different lasers are bundled adjacently in a tight-packed arrangement. The brightness can be increased by tapering the tips of the bundled fibers to match a single, multi-mode output fiber, e.g., one whose core that is just wide enough to fit the input cores. This increases the brightness of the beam combining. In addition, reducing the outer diameters of the signal fiber claddings allows the signal fibers to be bundled closer together, making it possible to couple more signal fiber cores to the core of a multi-mode output fiber. Similarly, reducing the outer diameter of the pump fiber cladding and/or etching away corresponding portions of the signal fiber cladding in a pump/signal combiner makes it possible to couple more pump light into the signal fiber cladding, again increasing brightness.

Abstract: Arrays of fiber pigtails can be used to project and receive light. Unfortunately, most fiber pigtail arrays are not aligned well enough for coherently combining different optical beams. This imprecision stems in part from misalignment between the optical fiber and the endcap spliced to the end of the optical fiber. The endcap is often polished, curved, or patterned, causing the light emitted by the endcapped fiber to refract or diffract as it exits the endcap. This refraction or diffraction shifts the apparent position of the beam waist from its actual position. Measuring this virtual beam waist position before and after splicing the endcap to the fiber increases the absolute precision with which the fiber is aligned to the endcap. This increase in absolute precision reduces the deviation in virtual beam waist position among endcapped fibers, making it easier to produce arrays of endcapped fibers aligned precisely enough for coherent beam combining.

Abstract: Provided herein are recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A proteins) with an altered (e.g., improved) glycosylation profile, and pharmaceutical compositions and kits including one or more of these proteins. Also provided are methods of generating a mammalian cell useful for recombinant expression of a glycoprotein (e.g., recombinant human ?-galactosidase-A), methods of producing recombinant glycoproteins, and methods of treatment that include administering to a subject at least one of the recombinant glycoproteins (e.g., recombinant human ?-galactosidase-A protein).

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.