Abstract: A method of target molecule detection includes simultaneously obtaining a first signal from a first working electrode and a second signal from a second working electrode, wherein the first signal is responsive to interaction of the first recognition element with the target molecule in a sample, and the second signal is indicative of background noise from the sample. The method further includes generating a modified signal that is proportional to an instantaneous difference between the first and second signals, wherein the modified signal indicates an amount of the target molecule present in the sample.

Abstract: Described herein are DNA-nanostructures that can be used in an assay to detect and/or quantify an analyte of interest. Aspects of the DNA-nanostructure can include a single DNA molecule composed of hairpin structural motifs, an anchor recognition moiety, and a signal moiety, where the anchor recognition moiety and the signal moiety are in effective proximity to each other such that the tethered diffusion of the signal molecule can be altered based upon binding status of the anchor recognition moiety. Also described herein are methods of making and using the DNA-nanostructures.

Abstract: Described herein are DNA-nanostructures that can be used in an assay to detect and/or quantify an analyte of interest. Aspects of the DNA-nanostructure can include a single DNA molecule composed of hairpin structural motifs, an anchor recognition moiety, and a signal moiety, where the anchor recognition moiety and the signal moiety are in effective proximity to each other such that the tethered diffusion of the signal molecule can be altered based upon binding status of the anchor recognition moiety. Also described herein are methods of making and using the DNA-nanostructures.

Abstract: A method of target molecule detection includes simultaneously obtaining a first signal from a first working electrode and a second signal from a second working electrode, wherein the first signal is responsive to interaction of the first recognition element with the target molecule in a sample, and the second signal is indicative of background noise from the sample. The method further includes generating a modified signal that is proportional to an instantaneous difference between the first and second signals, wherein the modified signal indicates an amount of the target molecule present in the sample.

Abstract: Described herein are DNA-nanostructures that can be used in an assay to detect and/or quantify an analyte of interest. Aspects of the DNA-nanostructure can include a single DNA molecule composed of hairpin structural motifs, an anchor recognition moiety, and a signal moiety, where the anchor recognition moiety and the signal moiety are in effective proximity to each other such that the tethered diffusion of the signal molecule can be altered based upon binding status of the anchor recognition moiety. Also described herein are methods of making and using the DNA-nanostructures.

Abstract: Provided herein are compositions and methods including the step of thermally scanning a sample that can be used and implemented to detect the presence of and/or concentration of a molecule in a sample.

Abstract: Provided herein are compositions and methods including the step of thermally scanning a sample that can be used and implemented to detect the presence of and/or concentration of a molecule in a sample.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: The present invention relates to microfluidic systems and methods for controlling the flow of fluid using passive components engineered into the microchannels. These passive flow components include fluidic diodes, fluidic capacitors, and fluidic inductors. Various fluidic circuits are provided to control fluid flow including fluid rectifiers, fluid band pass filters, and fluid timers.

Abstract: The present invention provides an integrated microfluidic analysis system. The system contains at least a first (pre-reaction treatment) domain for treating a sample prior to subjecting the sample to a chemical reaction. The following domains are optionally added to the first domain: a second (reaction) domain for reacting the chemical of interest in the sample; and a third (post-reaction separation) domain for separating products and reactants coming out of the reaction domain. The integrated microfluidic analysis system of the present invention is most applicable to PCR analysis.

Abstract: The present disclosure includes an electrochemical proximity assay (ECPA) which leverages two aptamer or antibody-oligonucleotide probes and proximity-dependent DNA hybridization to move a redox active molecule near an electrically conductive base. The ECPA of the present disclosure produces rapid, quantitative results, enabling point-of-care use in the detection of biomarkers of disease.

Abstract: The present invention relates to microfluidic systems and methods for controlling the flow of fluid using passive components engineered into the microchannels. These passive flow components include fluidic diodes, fluidic capacitors, and fluidic inductors. Various fluidic circuits are provided to control fluid flow including fluid rectifiers, fluid band pass filters, and fluid timers.

Abstract: The present invention relates to microfluidic systems and methods for controlling the flow of fluid using passive components engineered into the microchannels. These passive flow components include fluidic diodes, fluidic capacitors, and fluidic inductors. Various fluidic circuits are provided to control fluid flow including fluid rectifiers, fluid band pass filters, and fluid timers.

Abstract: The present invention relates to microfluidic systems and methods for controlling the flow of fluid using passive components engineered into the microchannels. These passive flow components include fluidic diodes, fluidic capacitors, and fluidic inductors. Various fluidic circuits are provided to control fluid flow including fluid rectifiers, fluid band pass filters, and fluid timers.

Abstract: The present invention provides an integrated microfluidic analysis system. The system contains at least a first (pre-reaction treatment) domain for treating a sample prior to subjecting the sample to a chemical reaction. The following domains are optionally added to the first domain: a second (reaction) domain for reacting the chemical of interest in the sample; and a third (post-reaction separation) domain for separating products and reactants coming out of the reaction domain. The integrated microfluidic analysis system of the present invention is most applicable to PCR analysis.

Abstract: The present invention relates to microfluidic devices (20), and in particular, heat management in such devices. To achieve desired thermal properties in selected areas of a microfluidic or nanofluidic device, selective removal or addition of material (thermal mass) can be effected in certain selected regions of the device to control thermal properties, wherein the selected regions are immediately surrounding a reaction chamber (14) and resulting in an empty space (18). This is particularly useful in accommodating rapid heating and/or cooling rates during sample processing and analysis on a microfluidic or nanofluidic device.