Abstract: Disclosed is an automated tangential flow filtration (TFF) perfusion bioreactor for culturing eukaryotic cells, useful for manufacturing a purified protein of interest, such as, but not limited to, a recombinant or naturally occurring protein and/or a therapeutic or other medically useful protein. The disclosed TFF perfusion bioreactor system can operate at high cell densities for long production cultivation periods (13-90 days) that provide the greatest efficiencies, whether in batch mode, continuous, or semi-continuous biologics manufacturing platforms.

Abstract: A method of and system for determining calibration offsets to account for delays introduced “downstream” of the reference driver (24) of a tester system (18) to test electronic components such as SRAM semiconductor memory devices. Such delays are created by, among other elements, receiver channels (30) of the tester system. A plurality of calibration modules (100) are provided, one for each receiver channel. Each calibration modules has a transmission line (110) with a known delay, a first contact 102′ and a second contact 102″. The tester system includes a socket (52) having a plurality of contactors (54) for contacting the reference clock contact and data output contacts of the electronic components undergoing test. The first contact of each calibration module is positioned to engage the contactor that engages the reference clock contact of the electronic component.