Abstract: A methodology scales supercritical fluid chromatography (SFC) and/or carbon dioxide based chromatography methods between different system and/or column configurations. The methodology includes measuring an average mobile phase density during a first separation utilizing C02 as a mobile phase component and substantially duplicating the average density profile for a second separation. Substantial duplication of the average mobile phase density (e.g., within about 10%, 5%, 2.5%, 1%, 0.5%, 0.1 %, 0.05%) results in chromatography for both system and/or column configurations having similar selectivity and retention factors. Average mobile phase density may be, either measured directly, calculated, or approximated using average pressure or density measurements. The average pressure profile may be used as a close approximation to duplicate average density profiles between separations.

Abstract: The present disclosure relates to methodologies, systems, and devices for discretely collecting extracts from an extract collection container of a CO2-based extraction system. A removable liner can be placed within the extract collection container such that the extract is collected within the removable liner, which can be removed or collected at discrete times. The removable liner can be hung from an extract fluid inlet tube or attached to an internal sidewall of the extract collection container.

Abstract: The subject technology is directed to a CO2-based chromatography system and method for rapid determination of the levels and/or the presence or absence of steroids or steroid derivatives in a sample.

Abstract: The subject technology is directed to a CO2-based chromatography system and method for rapid determination of the levels and/or the presence or absence of steroids or steroid derivatives in a sample.