Abstract: Provided herein are compositions and methods for co-production and recovery of two or more isoprenoids from a single recombinant cell.

Abstract: The present disclosure relates to method of obtaining high purity glucans, in particular yeast glucans with immunomodulatmy properties, including vaccine adjuvant properties for the use as adjuvant in vaccines.

Abstract: Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme.

Abstract: Provide are modified host cells that are engineered to decrese expression of a product to undetectable levels in the presence of an exogenous agent, and increase expression of the product in the presence of another exogenous agent. The modified yeast strainshost cells do not express detectable levels of a precursor or substrate used to make the product. The product can be a cannabinoid or precursor thereof, and the substrate can be hexanoate. Also provided are methods for making a product using the modified host cells. The modified host cell can be a yeast strain, such as S. cerevisiae.

Abstract: Provided herein are compositions and methods for co-production and recovery of two or more isoprenoids from a single recombinant cell.

Abstract: The invention provides several new transporter proteins, nucleic acids encoding them and methods of using the transporters to screen agents, conjugates or conjugate moieties, linked or linkable to agents, for capacity to be transported as substrates through the transporters. The invention also provides methods of treatment involving oral delivery of agents that either alone, or as a result of linkage to a conjugate moiety, are substrates of one of the transporter.

Abstract: Methods, compositions, and kits are provided for detecting binding between two members of a specific binding pair. The subject methods employ specific binding members conjugated to complementing RNAse domains to form binding member-complementing domain conjugates. When the specific binding members bind to each other, the members of the complementing RNAse domains are brought into non-covalent contact and reconstitute an active RNAse enzyme by complementation. The subject methods find use in both binding assays and competitive binding assays.

Abstract: The invention provides methods for screening libraries of complexes for compounds having a desired property, especially, the capacity to agonize, bind to, or antagonize a cellular receptor. The complexes in such libraries comprise a compound under test, a tag recording at least one step in synthesis of the compound, and a tether susceptible to modification by a reporter molecule. Modification of the tether is used to signify that a complex contains a compound having a desired property. The tag can be decoded to reveal at least one step in the synthesis of such a compound.