Abstract: A system for concentrating cells, wherein a syringe comprises a lumen and an axial end comprising a port and a radial end closed to liquid flow. A plunger divides the axial and radial ends, and the syringe is configured to hold a cellular suspension. A filter disposed at the radial end is configured to maintain sterility of the syringe. A cap comprises a vent disposed at the radial end. The plunger is configured to be actuated towards the axial end by air pressure being applied into the radial end and the plunger is configured to be actuated towards the radial end by a vacuum being applied into the radial end.

Abstract: A pneumatic driver for a syringe is provided. The syringe has an elongated barrel with a cap secured to the proximal end that has a through bore. A filter is secured to the cap interior of the barrel that covers an opening in the through bore interior of the barrel. The pneumatic driver includes a source of pressurized air; a support for receiving and locating the syringe; and a supply block configured to receive pressurized air from the source and having an outlet for delivery of pressurized air. The supply block is movable between a first position spaced apart from the cap of the syringe and a second position in contact with the cap with fluid communication being established between the outlet of the supply block and the through-bore in the cap.

Abstract: A priming subsystem of a cell processing system carries out a method for priming a spinning membrane separator having an inlet and first and second outlets. The method includes opening a first selectable junction disposed between a priming fluid container and the inlet to open a path between the priming fluid container and the inlet, operating a first pump coupled to the first outlet to draw priming fluid from the priming fluid container into the spinning membrane separator, and closing the first selectable junction and a second selectable junction coupled to the second outlet after drawing the priming fluid into the spinning membrane separator. The method further includes operating the first pump after the first selectable junction is closed to draw a vacuum, and opening the first selectable junction after drawing the vacuum.

Abstract: A method of processing blood includes receiving from a touch screen user input data for a protocol for a wash procedure. The user input data includes a numeric value. The method includes storing the protocol in a memory, receiving an identifier from a user, determining if the identifier permits the user to modify the protocol and receiving a modification of the protocol from the user. The method includes providing a plurality of authorizations, a second authorization being a default and an administrator authorization permitting the user to change a setting. The method includes comparing a password to a password stored in memory and, if there is a match, determining that the password is associated with an administrator. If a command to change the setting is received, the command is applied to change the setting. The blood is processed employing a wash procedure using the modified protocol and the changed setting.

Abstract: A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, first and second containers selectively connected to the first outlet; and a magnet. The system also includes a controller configured to operate the spinning membrane to receive biological fluid and to direct the target cells to the first container, to pause to permit magnetic particles to be associated with the target cells in the first container, to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles, to remove or deactivate the magnet, and to transfer the target cells to the second container.

Abstract: The present disclosure relates to systems and methods for the separation of blood into blood products and, more particularly, to systems and methods that permit automated recovery of white blood cells after producing a leukocyte-reduced blood product.

Abstract: A variety of fill options is provided for a cell processing system. Certain options relate to a filling system associated with the cell processing system, the filling system comprising one or more filling stations, each with at least one container that may receive product from a container associated with a cell processing system. Other options relate to a syringe assembly that receives a product from a container associated with a cell processing system.

Abstract: Systems, devices and methods for establishing a sterile fluid flow path between two or more fluid processing sets are disclosed. The systems, devices and methods include two or more connectors to which tubes of two or more fluid processing sets are attached. The connectors are brought together in an air-tight, non-permanent engagement with one another and treated with sterilizing light.

Abstract: A device, system, and method for sterilizing a fluid pathway connection are disclosed. The device, system, and method utilize a hand-held sterilization device. The device may create temporary sterile flow paths.

Abstract: A cell processing system includes a processor connectable to a source container filled with a biological fluid, the processor including a separator configured to separate the biological fluid from the source container into at least two streams according to a process including at least one process parameter, and a controller coupled to the processor and an input. The controller is configured to receive the at least one process parameter, to evaluate the process using the at least one process parameter before performing the process, and to carry out one or more actions based on the evaluation, such as providing an output estimate to the operator, preventing the process from being performed according to a comparison between a calculated condition and a control, or providing an error indication to the operator according to the calculated condition and a measured in-process condition.

Abstract: A fluid processing cassette includes first and second covers, with an interior wall positioned between the covers. The interior wall includes a first surface facing the first cover and defining a portion of a plurality of macrofluidic channels. A second surface of the interior wall faces the second cover and defines a portion of a plurality of microfluidic channels. At least one opening defined in the interior wall provides fluid communication between at least one of the macrofluidic channels and at least one of the microfluidic channels. An additional interior wall may also be positioned between the covers, with each interior wall secured to a different one of the covers and to the other interior wall. The additional interior wall may be positioned between the first cover and the interior wall, with macrofluidic channels defined on each side of the additional interior wall.

Abstract: A method for prophylaxis or treatment of a graft's rejection of a recipient, driven and adjusted by a microprocessor-based controller. Provided is a fluid circuit comprising a first container configured to receive a transplant component and a second container configured to receive an apoptotic component. Provided is a separator configured to associate with the fluid circuit and separate whole blood into a red blood cell component, a plasma component, and a white blood cell component. Whole blood is directed into the fluid circuit and the separator. The whole blood is separated into the red blood cell component, the plasma component, and the white blood cell component. A first portion comprising the transplant component of the white blood cell component is directed to the first container. A second portion of the white blood cell component is directed to the second container and the second portion is rendered apoptotic.

Abstract: A cell processing system includes a processor connectable to a source container filled with a biological fluid, the processor including a separator configured to separate the biological fluid from the source container into at least two streams according to a process including at least one process parameter, and a controller coupled to the processor and an input. The controller is configured to receive the at least one process parameter, to evaluate the process using the at least one process parameter before performing the process, and to carry out one or more actions based on the evaluation, such as providing an output estimate to the operator, preventing the process from being performed according to a comparison between a calculated condition and a control, or providing an error indication to the operator according to the calculated condition and a measured in-process condition.

Abstract: Systems and methods are provided for separating blood components from whole blood. A blood processing device, as part of a blood processing system, has a controller configured to operate the blood processing device based on a plurality of collection protocols. The blood processing device is wirelessly connected to a remote computing device configured to maintain a plurality of collection protocols. At least one additional computing device is configured to update one or more of the plurality of collection protocols on said remote computing device by communicating changes to a protocol maintained on the remote computing device which updates the plurality of collection protocols at the controller of the blood processing device.

Abstract: A method of operating a magnetic selector is provided. The magnetic selector includes a housing including a floor having a floor surface, a magnet carrier disposed on an opposite side of the floor from the floor surface and having at least one magnet disposed thereon, the magnet carrier being moveable relative to the floor between a first state wherein the magnet carrier is adjacent the floor and a second state wherein the magnet carrier is spaced from the floor. The method includes disposing a container on the floor surface, moving the magnet carrier to the first state to apply a magnetic field to a fluid in the container, moving the magnet carrier to the second state from the first state to disengage the magnetic field from the container, and removing the container.

Abstract: A magnetic selector includes a housing configured to receive a container and having a floor upon which the container is disposed. The selector also includes a magnet carrier disposed on an opposite side of the floor from the container. The magnet carrier has at least one magnet disposed thereon and being moveable relative to the floor between a first state wherein the magnet carrier is adjacent the floor and a second state wherein the magnet carrier is spaced from the floor. The selector may include a door. The magnetic selector is usable in a fluid processing system that includes a fluid processor connectable to a source container filled with a biological fluid. A container is disposed in the magnetic selector and connected to the processor along a fluid pathway. The processor is configured to separate the biological fluid from the source container into at least two streams of material.

Abstract: A fluid processing system may include a flow control cassette comprising at least one interface sensor chamber in fluid communication with at least one of a plurality of separate channels, the at least one interface sensor chamber defined at least in part by a wall, and at least one capacitive sensor disposed on the wall of the at least one interface sensor chamber. The fluid processing system may include, in the alternative or in addition, at least one syringe comprising a wall defining a barrel having a first end and a second end, the barrel having a bore with or without a piston or plunger disposed therein, and at least one capacitive sensor disposed on an outer surface of the wall of the syringe.

Abstract: A fluid processing system includes a disposable fluid circuit and reusable hardware configured to accept the disposable fluid circuit. The disposable fluid circuit includes a spinning membrane separator, first and second syringes, and a flow control cassette. The reusable hardware includes a spinning membrane separator drive coupled to the spinning membrane separator, first and second syringe pumps coupled to the first and second syringes respectively, a control cassette interface coupled to the flow control cassette, and at least one controller coupled to the spinning membrane separator drive, the first and second syringe pumps, and the control cassette interface. The controller is configured to selectively operate the drive, the first and second syringe pumps, and the interface to provide a procedure according to a protocol.

Abstract: A priming subsystem of a cell processing system carries out a method for priming a spinning membrane separator having an inlet and first and second outlets. The method includes opening a first selectable junction disposed between a priming fluid container and the inlet to open a path between the priming fluid container and the inlet, operating a first pump coupled to the first outlet to draw priming fluid from the priming fluid container into the spinning membrane separator, and closing the first selectable junction and a second selectable junction coupled to the second outlet after drawing the priming fluid into the spinning membrane separator. The method further includes operating the first pump after the first selectable junction is closed to draw a vacuum, and opening the first selectable junction after drawing the vacuum.

Abstract: A system for washing red blood cells, comprising a separator configured to separate a quantity of blood into concentrated red blood cells having a hematocrit of at least 60% and a volume of 150-250 mL, and a supernatant component. The system comprises a flow controller configured to remove the supernatant component to provide an initial red blood cell concentrate; combine 50-500 mL of an additive solution with the red blood cell concentrate to provide an intermediate red blood cell product intended for storage for 42 days or less; and wash the intermediate red blood cell product with a washing solution.