Abstract: An object support includes an object receiving portion extending from a panel and configured to support an object, with the object receiving portion configured to move relative to an interior surface between a first position when the panel is in a closed position and a second position when the panel is in an open position. The object receiving portion is closer to a lateral surface of the panel in the second position than the first position.

Abstract: Aspects of the present disclosure include methods for processing and displaying multi-channel spectral histograms. Methods according to certain embodiments include obtaining a histogram of cytometric data for a sample, wherein the cytometric data comprises measurements from particles irradiated in the sample flowing in a flow stream, generating the representation of the histogram by encoding the histogram, the encoding comprising: assigning a color to each histogram value in the representation of the histogram, and duplicating each color corresponding to each histogram value in the representation of the histogram a predetermined number of times, and using the representation of the histogram for displaying the histogram. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.

Abstract: Aspects of the present disclosure include methods for dynamic real-time adjustment of data acquisition parameters of a particle analyzer. Methods according to certain embodiments include detecting light from a particle of a sample in a flow stream irradiated with a light source, generating an image of the particle based on the detected light and automatically adjusting a data acquisition parameter of the particle analyzer in response to a modulated visualization parameter for the image of the particle. Systems (e.g., particle analyzers) having a light source and a light detection system that includes an imaging photodetector and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

Abstract: Methods of classifying flow cytometer data are provided. Methods of interest include receiving a first gate and flow cytometer data, expanding the first gate to generate a second gate, and determining sets of flow cytometer data encompassed by each of the first gate and the second gate to classify the flow cytometer data. In embodiments, methods also involve recording a subset of the classified flow cytometer data and optionally adjusting the first and/or second gates based on the recorded data. In some cases, the subject methods include sorting particles associated with the classified flow cytometer data based on the first and second gates. Systems and computer-readable storage media for practicing the invention are also provided.

Abstract: Disclosed herein are methods for producing high density cellular arrays. In some embodiments, the methods comprise: providing a sample comprising a plurality of cells; and introducing the plurality of cells in the sample into microwells of a microwell array to produce a cellular array, wherein the microwell array comprises 500 or more microwells per inch2, and wherein 25% or more of the microwells of the cellular array comprise a single cell. The disclosed methods can be used for producing a high density synthetic particle array and a high density reagent array.

Abstract: Aspects of the present disclosure include methods for processing and displaying multi-channel spectral histograms. Methods according to certain embodiments include obtaining a histogram of cytometric data for a sample, wherein the cytometric data comprises measurements from particles irradiated in the sample flowing in a flow stream, generating the representation of the histogram by encoding the histogram, the encoding comprising: assigning a color to each histogram value in the representation of the histogram, and duplicating each color corresponding to each histogram value in the representation of the histogram a predetermined number of times, and using the representation of the histogram for displaying the histogram. Systems for practicing the subject methods are also provided. Non-transitory computer readable storage mediums are also described.

Abstract: Some embodiments include an apparatus for processing radio frequency multiplexed cell images such as single-cell images, for example radio frequency multiplexed excitation (FIRE) images. The images can be from a particle analyzer, such as a flow and scanning cytometer or a laser scanning microscope. Some embodiments include an apparatus for displaying radio frequency multiplexed cell images such as FIRE images.

Abstract: Features for a quantitative biological data analysis system are described. Index sort analysis can be performed using sort electronics on a flow cytometer or other particle analysis system or after an experiment using a workstation. The sort electronics on the cytometer or other particle analysis system may generate sort decision information for the events. The sort decision information may be transmitted from the sort electronics to the workstation that may differ from the sort identified by the workstation. The feature fuse the gate information with the sorting decision information to provide an accurate representation of the sorting.

Abstract: Systems and methods that provide a multitude of new ways to interact with the data are provided. One purpose of index sort analysis may be to visualize where particular cells are in the plate device and on the bi-variate plots. The user can ‘select’ particular cell events either by clicking on the plate wells or by clicking and selecting an area of interest on the plots. The corresponding selection of cell events may then then be coordinated to maintain a consistent representation of the events on visualizations (e.g., one or more user interfaces).

Abstract: Some embodiments include an apparatus for processing radio frequency multiplexed cell images such as single-cell images, for example radio frequency multiplexed excitation (FIRE) images. The images can be from a particle analyzer, such as a flow and scanning cytometer or a laser scanning microscope. Some embodiments include an apparatus for displaying radio frequency multiplexed cell images such as FIRE images.

Abstract: Some embodiments include an apparatus for processing radio frequency multiplexed cell images such as single-cell images, for example radio frequency multiplexed excitation (FIRE) images. The images can be from a particle analyzer, such as a flow and scanning cytometer or a laser scanning microscope. Some embodiments include an apparatus for displaying radio frequency multiplexed cell images such as FIRE images.

Abstract: Aspects of the present disclosure include methods for generating a bitmap from a data plot of light detected from particles in a flow stream. Methods according to certain embodiments include detecting light from particles in a flow stream, generating a data plot of measurements of the detected light, where the data plot includes one or more regions each having a population of particles, calculating a set of vertices that form a boundary for each region in the data plot, identifying a type of algorithmic transformation associated with each vertex in the set of vertices, generating a bitmap of each region of particles such that the bitmap of each region includes a set of vertices that correspond to the vertices of each region in the data plot and identifying an algorithmic transformation for applying to each vertex in the bitmap of each region. Systems and integrated circuit devices (e.g.

Abstract: Aspects of the present disclosure include methods for generating a bitmap from a data plot of light detected from particles in a flow stream. Methods according to certain embodiments include detecting light from particles in a flow stream, generating a data plot of measurements of the detected light, where the data plot includes one or more regions each having a population of particles, calculating a set of vertices that form a boundary for each region in the data plot, identifying a type of algorithmic transformation associated with each vertex in the set of vertices, generating a bitmap of each region of particles such that the bitmap of each region includes a set of vertices that correspond to the vertices of each region in the data plot and identifying an algorithmic transformation for applying to each vertex in the bitmap of each region. Systems and integrated circuit devices (e.g.

Abstract: Some embodiments include an apparatus for processing radio frequency multiplexed cell images such as single-cell images, for example radio frequency multiplexed excitation (FIRE) images. The images can be from a particle analyzer, such as a flow and scanning cytometer or a laser scanning microscope. Some embodiments include an apparatus for displaying radio frequency multiplexed cell images such as FIRE images.

Abstract: Features for a quantitative biological data analysis system are described. Index sort analysis can be performed using sort electronics on a flow cytometer or other particle analysis system or after an experiment using a workstation. The sort electronics on the cytometer or other particle analysis system may generate sort decision information for the events. The sort decision information may be transmitted from the sort electronics to the workstation that may differ from the sort identified by the workstation. The feature fuse the gate information with the sorting decision information to provide an accurate representation of the sorting.

Abstract: Systems and methods that provide a multitude of new ways to interact with the data are provided. One purpose of index sort analysis may be to visualize where particular cells are in the plate device and on the bi-variate plots. The user can ‘select’ particular cell events either by clicking on the plate wells or by clicking and selecting an area of interest on the plots. The corresponding selection of cell events may then then be coordinated to maintain a consistent representation of the events on visualizations (e.g., one or more user interfaces).

Abstract: Automated cell sorters are provided. Aspects of the systems include an image based automated stream adjuster. Also provided are methods of using the automated cell sorters.

Abstract: Disclosed herein are methods for producing high density cellular arrays. In some embodiments, the methods comprise: providing a sample comprising a plurality of cells; and introducing the plurality of cells in the sample into microwells of a microwell array to produce a cellular array, wherein the microwell array comprises 500 or more microwells per inch2, and wherein 25% or more of the microwells of the cellular array comprise a single cell. The disclosed methods can be used for producing a high density synthetic particle array and a high density reagent array.