Patents by Inventor Christopher L. Baszczynski

Christopher L. Baszczynski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9222098
    Abstract: Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site.
    Type: Grant
    Filed: August 12, 2003
    Date of Patent: December 29, 2015
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 8735158
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: February 28, 2008
    Date of Patent: May 27, 2014
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 8536420
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: August 1, 2008
    Date of Patent: September 17, 2013
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 8143504
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: October 22, 2010
    Date of Patent: March 27, 2012
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7987735
    Abstract: The present device enables measurement of the susceptibility of corn plants to root lodging. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to root lodging. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.
    Type: Grant
    Filed: October 12, 2009
    Date of Patent: August 2, 2011
    Assignees: Iowa State University Research Foundation, Inc., Pioneer Hi-Bred International, Inc.
    Inventors: Julian Adin Mann, III, Ashli J. Armstrong, Roberto Barreiro, Christopher L. Baszczynski, Terry EuClaire Meyer
  • Publication number: 20110083519
    Abstract: The present device enables measurement of the susceptibility of corn plants to stalk lodging and brittle snap. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to stalk lodging and brittle snap. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.
    Type: Application
    Filed: October 12, 2009
    Publication date: April 14, 2011
    Applicant: Pioneer Hi-Bred International
    Inventors: Roberto Barreiro, Christopher L. Baszczynski, Terry EuClaire Meyer
  • Publication number: 20110030107
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: October 22, 2010
    Publication date: February 3, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7820880
    Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
    Type: Grant
    Filed: November 6, 2008
    Date of Patent: October 26, 2010
    Assignee: Pioneer Hi-Bred Int'l. Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7807868
    Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.
    Type: Grant
    Filed: April 2, 2008
    Date of Patent: October 5, 2010
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Lesek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
  • Publication number: 20100089176
    Abstract: The present device enables measurement of the susceptibility of corn plants to root lodging. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to root lodging. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.
    Type: Application
    Filed: October 12, 2009
    Publication date: April 15, 2010
    Applicants: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC., PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: JULIAN ADIN MANN, III, ASHLI J. ARMSTRONG, ROBERTO BARREIRO, CHRISTOPHER L. BASZCZYNSKI, TERRY EuCLAIRE MEYER
  • Patent number: 7572634
    Abstract: Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites. Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene. By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.
    Type: Grant
    Filed: May 7, 2003
    Date of Patent: August 11, 2009
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Publication number: 20090093059
    Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.
    Type: Application
    Filed: November 6, 2008
    Publication date: April 9, 2009
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Publication number: 20080320617
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: August 1, 2008
    Publication date: December 25, 2008
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Patent number: 7462766
    Abstract: Compositions comprising non-identical recombination sites are provided. Compositions include plants, plant parts and plant cells comprising transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites.
    Type: Grant
    Filed: May 4, 2006
    Date of Patent: December 9, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
  • Publication number: 20080282426
    Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.
    Type: Application
    Filed: April 2, 2008
    Publication date: November 13, 2008
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Christopher L. Baszczynski, Lesek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
  • Publication number: 20080209595
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Application
    Filed: February 28, 2008
    Publication date: August 28, 2008
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7405079
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: May 7, 2003
    Date of Patent: July 29, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7364902
    Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.
    Type: Grant
    Filed: April 7, 2006
    Date of Patent: April 29, 2008
    Assignee: Pioneer Hi-Bred International, Inc,
    Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
  • Patent number: 7361508
    Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
    Type: Grant
    Filed: May 16, 2003
    Date of Patent: April 22, 2008
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
  • Patent number: 7223601
    Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.
    Type: Grant
    Filed: January 29, 2003
    Date of Patent: May 29, 2007
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Aragula Gururaj Rao, Laura A. Tagliani