Patents by Inventor Christopher L. Baszczynski
Christopher L. Baszczynski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9222098Abstract: Compositions for the targeted integration of nucleotide sequences into a plant are provided. Plants and plant cells having multiple transfer cassettes stacked at precise locations in the genome of a plant or plant cell are provided. In specific embodiments, the plant or plant cell comprises at least two transfer cassettes. The first transfer cassette comprises a first nucleotide sequence of interest flanked by a first and a second recombination site, wherein the first and the second recombination sites are non-identical. The second transfer cassette comprises a second nucleotide sequence of interest flanked by the second recombination site and a third recombination site, wherein the third recombination site is non-identical to the first and said second recombination site.Type: GrantFiled: August 12, 2003Date of Patent: December 29, 2015Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Patent number: 8735158Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: GrantFiled: February 28, 2008Date of Patent: May 27, 2014Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Patent number: 8536420Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: GrantFiled: August 1, 2008Date of Patent: September 17, 2013Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
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Patent number: 8143504Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: GrantFiled: October 22, 2010Date of Patent: March 27, 2012Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
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Patent number: 7987735Abstract: The present device enables measurement of the susceptibility of corn plants to root lodging. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to root lodging. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.Type: GrantFiled: October 12, 2009Date of Patent: August 2, 2011Assignees: Iowa State University Research Foundation, Inc., Pioneer Hi-Bred International, Inc.Inventors: Julian Adin Mann, III, Ashli J. Armstrong, Roberto Barreiro, Christopher L. Baszczynski, Terry EuClaire Meyer
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Publication number: 20110083519Abstract: The present device enables measurement of the susceptibility of corn plants to stalk lodging and brittle snap. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to stalk lodging and brittle snap. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.Type: ApplicationFiled: October 12, 2009Publication date: April 14, 2011Applicant: Pioneer Hi-Bred InternationalInventors: Roberto Barreiro, Christopher L. Baszczynski, Terry EuClaire Meyer
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Publication number: 20110030107Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: ApplicationFiled: October 22, 2010Publication date: February 3, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
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Compositions and methods to stack multiple nucleotide sequences of interest in the genome of a plant
Patent number: 7820880Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.Type: GrantFiled: November 6, 2008Date of Patent: October 26, 2010Assignee: Pioneer Hi-Bred Int'l. Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani -
Patent number: 7807868Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: April 2, 2008Date of Patent: October 5, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Lesek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Publication number: 20100089176Abstract: The present device enables measurement of the susceptibility of corn plants to root lodging. The device is used to push over a corn stalk and the force used to push over the stalk, and the vibration of the stalk caused by the push are recorded. As material breaks in the stalk, an accelerometer, measures stalk vibration response to the breaking events; the data is then recorded to allow quantitative measurements of the susceptibility of corn plants to root lodging. This allows meaningful comparisons of various hybrids at early stages of hybrid evaluation and advancement.Type: ApplicationFiled: October 12, 2009Publication date: April 15, 2010Applicants: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC., PIONEER HI-BRED INTERNATIONAL, INC.Inventors: JULIAN ADIN MANN, III, ASHLI J. ARMSTRONG, ROBERTO BARREIRO, CHRISTOPHER L. BASZCZYNSKI, TERRY EuCLAIRE MEYER
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Patent number: 7572634Abstract: Methods to find optimal integration sites within a plant genome are provided. More particularly, a plant is transformed with a target site having an expression cassette comprising a nucleotide sequence operably linked to a promoter active in the plant. The target site is flanked by non-identical recombination sites. Transformed protoplast, tissues, or whole plants can be tested to determine the levels of activity of the inserted gene. By comparison of cellular activities of the gene in different insertion sites, preferred integration sites may be found wherein the gene is expressed at high or acceptable levels. These plants can then be utilized with subsequent retargeting techniques to replace the nucleotide sequence with other genes or nucleotide sequences of interest contained in a transfer cassette.Type: GrantFiled: May 7, 2003Date of Patent: August 11, 2009Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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COMPOSITIONS AND METHODS TO STACK MULTIPLE NUCLEOTIDE SEQUENCES OF INTEREST IN THE GENOME OF A PLANT
Publication number: 20090093059Abstract: Methods and compositions for the stacking of multiple nucleotide sequences at precise locations in the genome of a plant or plant cell are provided. Specifically, transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are introduced into a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase. The transfer cassettes and target sites are designed so as to allow for the stacking or ordering of nucleotide sequences at precise locations in the plant genome.Type: ApplicationFiled: November 6, 2008Publication date: April 9, 2009Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani -
Publication number: 20080320617Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: ApplicationFiled: August 1, 2008Publication date: December 25, 2008Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
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Patent number: 7462766Abstract: Compositions comprising non-identical recombination sites are provided. Compositions include plants, plant parts and plant cells comprising transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites.Type: GrantFiled: May 4, 2006Date of Patent: December 9, 2008Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura A. Tagliani
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Publication number: 20080282426Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: ApplicationFiled: April 2, 2008Publication date: November 13, 2008Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Christopher L. Baszczynski, Lesek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Publication number: 20080209595Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: ApplicationFiled: February 28, 2008Publication date: August 28, 2008Applicant: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Patent number: 7405079Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: GrantFiled: May 7, 2003Date of Patent: July 29, 2008Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Patent number: 7364902Abstract: The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell.Type: GrantFiled: April 7, 2006Date of Patent: April 29, 2008Assignee: Pioneer Hi-Bred International, Inc,Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan
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Patent number: 7361508Abstract: Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.Type: GrantFiled: May 16, 2003Date of Patent: April 22, 2008Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Patent number: 7223601Abstract: Compositions and methods for introducing a DNA of interest into a genomic target site are provided. In particular, the methods and compositions involve the use of a combination of target sites for two site specific recombinases and expression of a chimeric recombinase with dual target site specificity. Thus, the compositions comprise novel site-specific recombinases with specificities to multiple target sites, and nucleotide sequences and expression cassettes encoding these recombinases or target sites. The methods involve transforming a eukaryotic cell having target sites for the novel recombinase with a DNA of interest that is flanked by corresponding target sites. Expression of the recombinase results in integration of the DNA of interest into the genome of the cell. The compositions and methods of the invention have use in the construction of stably transformed eukaryotic cells, and in particular, plant cells.Type: GrantFiled: January 29, 2003Date of Patent: May 29, 2007Assignee: Pioneer Hi-Bred International, Inc.Inventors: Christopher L. Baszczynski, Leszek Alexander Lyznik, William J. Gordon-Kamm, Xueni Guan, Aragula Gururaj Rao, Laura A. Tagliani