Patents by Inventor Christopher L. Sigua

Christopher L. Sigua has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
    Patent number: 5693517
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
    Type: Grant
    Filed: February 2, 1995
    Date of Patent: December 2, 1997
    Assignee: Roche Molecular Systems, Inc.
    Inventors: David H. Gelfand, Thomas W. Myers, Christopher L. Sigua
  • Methods for coupled high temperatures reverse transcription and polymerase chain reactions
    Patent number: 5561058
    Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
    Type: Grant
    Filed: May 24, 1995
    Date of Patent: October 1, 1996
    Assignee: Hoffmann-La Roche Inc.
    Inventors: David H. Gelfand, Thomas W. Myers, Christopher L. Sigua