Abstract: Disclosed herein are DNA origami nanostmcture that can be functionalized for personalized and targeted drug delivery. The disclosed nanostructures enter cells through the endolysosomal pathway and circumvent drug resistance mechanisms in target cells. The disclosed nanostructures can be loaded small molecule drugs (e.g. anthracyclines, anti-metabolites) and nucleic acids (e.g. antisense oligonucleotides, siRNA, miRNA) with targeting and/or therapeutic antibodies against tumor-specific antigens (e.g. anti-CD33, anti-CD20) that can be modified to treat to a wide range of cancers and ultimately tailored to specific patients' needs for precision medicine.

Abstract: Disclosed herein are methods and compositions for enhancing delivery and function of vaccine components, immunotherapy Agents, and improved delivery of nucleic acid nanostructures, nucleic acids, peptides, polypeptides, and other types of cargoes. These methods and compositions utilize design components suitable for rapid and cost-effective manufacturing, and are designed to exclusively use the process of self-assembly to form nanotherapeutics requiring no purification in many instances.

Abstract: Disclosed herein is a vaccine device that involves a DNA origami nanostructure formed from a plurality of scaffold strands and a plurality of staple strands assembled into a rod shape, wherein a peptide antigen is attached to the DNA of the nanostructure by electrostatic interaction. Also disclosed herein is a method for vaccinating a subject that involves administering to the subject a therapeutically effective amount of a vaccine device disclosed herein.

Abstract: A pedal block may have an elongated quasi-cuboid structure characterized by a first foot-contacting surface on one face having a first front end and a first back end, and a second foot-contacting surface on an opposite face having a second front end and a second back end. A pedal spindle may be disposed in the pedal block between the first foot-contacting surface and the second foot-contacting surface and may include an axle and a crank attachment. The first foot-contacting surface and the second foot-contacting surface may each be contoured to contact a substantial portion of a foot of a rider whose foot is disposed on either the first foot-contacting surface or the second foot-contacting surface. A heel cup may be provided to support the heel and ankle of the rider.

Abstract: The present disclosure relates to biosensors and methods of use thereof.

Abstract: A steering system may include a seat assembly, a frame assembly and a wheel. The frame assembly may include a steering tube that is rotatably disposed within a head tube with a cam at a top end and a pair of forks at a bottom end to which is coupled an axle of the wheel. An arm may be disposed on and rotatably coupled to the seat assembly, and rotatably coupled to a left armrest and right armrest. A steering rod may be coupled to the cam and to one of the arms, the left armrest or the right armrest, such that forward movement of one of the armrests causes the steering rod to be translated forward and the cam to rotate in one direction, and backward movement of the same armrest causes the steering rod to be translated backward and the cam to rotate in an opposite direction.

Abstract: A steering system may include a seat assembly, a frame assembly and a wheel. The frame assembly may include a steering tube that is rotatably disposed within a head tube with a cam at a top end and a pair of forks at a bottom end to which is coupled an axle of the wheel. An arm may be disposed on and rotatably coupled to the seat assembly, and rotatably coupled to a left armrest and right armrest. A steering rod may be coupled to the cam and to one of the arms, the left armrest or the right armrest, such that forward movement of one of the armrests causes the steering rod to be translated forward and the cam to rotate in one direction, and backward movement of the same armrest causes the steering rod to be translated backward and the cam to rotate in an opposite direction.

Abstract: A valve assembly for an exhaust gas recirculation system includes a housing defining an inlet bore and including a second interior surface defining a housing bore. The valve assembly also includes a valve seat and a valve. The valve includes a valve stem and a valve sealing member. The valve assembly optionally includes a baffle at least partially disposed in the housing bore. A first interior radius is defined between the valve stem and the second interior surface. A second interior radius is defined between at least one of the valve stem and the second interior surface, the valve stem and the baffle, and the baffle and the second interior surface. The second interior radius is different from the first interior radius and is configured to direct the flow of the exhaust gas in the housing bore to reduce swirl of exhaust gas in the housing bore.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Disclosed herein are DNA origami nanostmcture that can be functionalized for personalized and targeted drug delivery. The disclosed nanostructures enter cells through the endolysosomal pathway and circumvent drug resistance mechanisms in target cells. The disclosed nanostructures can be loaded small molecule drugs (e.g. anthracyclines, anti-metabolites) and nucleic acids (e.g. antisense oligonucleotides, siRNA, miRNA) with targeting and/or therapeutic antibodies against tumor-specific antigens (e.g. anti-CD33, anti-CD20) that can be modified to treat to a wide range of cancers and ultimately tailored to specific patients' needs for precision medicine.

Abstract: Methods for performing inference on a generative model are provided. In one aspect, a method includes receiving a generative model in a probabilistic program form defining variables and probabilistic relationships between variables, and producing a neural network to model the behaviour of the generative model. The input layer includes nodes corresponding to the variables of the generative model, and the output layer includes nodes corresponding to a parameter of the conditional marginal of the variables of the input layer. The method also includes training the neural network using samples from the probabilistic program. A loss function is provided for each node of the output layer. The loss function for each output node is independent of the loss functions for the other nodes of the output layer. The method also includes performing amortised inference on the generative model. Systems and machine-readable media are also provided.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Methods for providing a computer implemented medical diagnosis are provided. In one aspect, a method includes receiving an input from a user comprising at least one symptom of the user, and providing the at least one symptom as an input to a medical model. The method also includes deriving estimates of the probability of the user having a disease from the discriminative model, inputting the estimates to the inference engine, performing approximate inference on the probabilistic graphical model to obtain a prediction of the probability that the user has that disease, and outputting the probability of the user having the disease for display by a display device.

Abstract: Children's ride-on vehicles having improved shifter assemblies. The vehicles include a drive assembly comprising a velocity control assembly that selectively configures the drive assembly within a plurality of drive configurations and which includes a switch assembly adapted to be selectively configured between a plurality of velocity settings. Each velocity setting configures the drive assembly to a predetermined drive configuration. The velocity control assembly may include an actuator assembly that receives user inputs via a shifter handle moveable between a plurality of shift positions along a plurality of shift paths. Each shift position configures the switch assembly to a particular velocity setting. At least two of the shift paths have non-linear relative orientations.

Abstract: An interactive gateway creates a centralized location that allows subscribers and non-subscribers to premium television channels to navigate interactive television features and added value content for the programming services available on the subscription channels. The programming services available on the subscription channels may include movies, original series, boxing and family programming. The interactive gateway can also introduce subscribers and non-subscribers to all of the channels and interactive services available from a particular content provider. Although both subscriber and non-subscribers can access the interactive gateway, their experiences may be different. Different content is preferably provided based on a viewer's subscription status. Non-subscribers are presented with promotion material regarding the programming services to entice non-subscribers to become subscribers.