Abstract: A method models and manages affinity networks. The method includes receiving real-time transaction data; processing a transaction of the real-time transaction data to identify a source node and a target node of a graph; and processing the transaction to update an affinity score of an edge from the source node to the target node. The method further includes receiving a request; selecting, responsive to the request, the target node using the affinity score after updating the affinity score; and presenting a response using the target node.

Abstract: Provided herein are methods of monitoring for the production of select antibodies in a non-human animal, comprising (a) immunizing a non-human animal with an immunogen; (b) obtaining a blood sample comprising antibody secreting cells (ASCs) from said non-human animal; and (c) individually assaying ASCs present in the blood sample, or a fraction thereof, for the production of select antibodies. Methods of guiding antibody production in a non-human animal for the production of select antibodies are also provided. In exemplary embodiments, the method comprises performing a cycle of (a) to (c), as above, and repeating the cycle when the percentage of ASCs producing select antibodies is below a threshold. In various aspects, the cycle is repeated until the percentage of ASCs producing select antibodies is at or above a threshold. Single cell assays are further provided herein.