Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue; an oxygenator for oxygenating perfusate that circulates through the perfusion circuit; and an oxygen supply device such as an oxygen concentrator or an oxygen generator configured to supply oxygen to the oxygenator. A method of perfusing an organ or tissue includes producing oxygen from a device such as an oxygen concentrator and an oxygen generator; supplying the produced oxygen, preferably as the oxygen is produced, to a perfusate to oxygenate the perfusate; and perfusing the organ or tissue with the oxygenated perfusate. The produced oxygen preferably has a concentration greater than the oxygen concentration in air.

Abstract: A temperature sensor for monitoring an organ or tissue is configured to measure a temperature inside of a container configured to contain the organ or tissue. The temperature sensor is disposed exterior to the organ container and the temperature sensor is a non-contact temperature sensor. The temperature sensor may be part of an apparatus for perfusing, transporting, and/or storing an organ or tissue. A coolant container may have an aperture through which the temperature sensor measures a temperature of at least one of the organ or tissue or a perfusate fluid surrounding the organ or tissue. The temperature sensor is preferably an infrared temperature sensor. Multiple temperature sensors may be provided that measure the temperature organ or tissue or perfusate fluid surrounding the organ or tissue, for example in case one of the temperature sensors fails.

Abstract: An organ container, which is for storing an organ or tissue and is able to be inserted into an apparatus for at least one of perfusion and transport of the organ or tissue, includes a basin configured to hold the organ or tissue and a perfusate bath. The organ container also includes tubing that (i) is connectable to a source of oxygen, (ii) includes a plurality of holes by which the oxygen may exit the tubing, and (iii) is located within the basin so as to be submerged within the perfusate bath present during the perfusion or transport of the organ or tissue.

Abstract: Disclosed is an oxygenator device for oxygenating a perfusate solution to be perfused through an organ or tissue. The device includes an inlet configured to receive oxygen from an oxygen supply; and tubing connected to the inlet, the tubing including a plurality of holes by which the received oxygen may exit the tubing.

Abstract: An apparatus for perfusing an organ or tissue includes a perfusion circuit for perfusing the organ or tissue; a controller configured to control the apparatus; and a detector configured to detect an optional component of the apparatus, and provide a signal to the controller indicative of the presence of the optional component, wherein the controller is configured to control the perfusion circuit in a first mode when the optional component is detected and to control the perfusion circuit in a second mode when the optional component is not detected.

Abstract: An organ perfusion apparatus may include at least two tubes connected to an organ or tissue. A method of perfusing an organ or tissue may include connecting a first end of each of the at least two tubes to an organ or tissue, applying a fluid motive force to a perfusion fluid in the two tubes to force the fluid through the two tubes into the organ or tissue, and perfusing the organ or tissue through the at least two tubes such that the fluid motive force, provided by, e.g., a pump, and backpressure generated by the organ or tissue, establishes a flow balance between the at least two tubes. The flow balance may be altered without altering the fluid motive force that is applied.

Abstract: Disclosed is a mobile cart for an organ container. The mobile cart includes a top assembly configured to be releasably secured to the organ container, which is at least one of an organ transporter and an organ perfusion apparatus configured to perfuse an organ. It also includes a support structure attached to the top assembly so as to support the top assembly, the support structure being moveable from an extended position to a collapsed position. The top assembly in turn includes distinct primary and secondary locking mechanisms, each of which is configured to secure the support structure in the extended position. And when the support structure is secured in the extended position, both the primary and secondary locking mechanisms must be actuated for the support structure to be moveable to the collapsed position.

Abstract: Disclosed is a mobile cart for an organ container. The mobile cart includes a top assembly configured to be releasably secured to the organ container, which is at least one of an organ transporter and an organ perfusion apparatus configured to perfuse an organ. It also includes a support structure attached to the top assembly so as to support the top assembly, the support structure being moveable from an extended position to a collapsed position. The top assembly in turn includes distinct primary and secondary locking mechanisms, each of which is configured to secure the support structure in the extended position. And when the support structure is secured in the extended position, both the primary and secondary locking mechanisms must be actuated for the support structure to be moveable to the collapsed position.

Abstract: An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a cooling container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment.

Abstract: A method of cannulating vasculature includes inserting the vasculature through a first clamping surface of a cannula and rotating a second clamping surface of the cannula around the first clamping surface to move from an open position towards a closed position. Additionally, the method includes securing the vasculature between the first clamping surface and the second clamping surface.

Abstract: A method of perfusing an organ or tissue includes perfusing the organ or tissue with a perfusion apparatus; detecting a condition representing at least one of an attitude of the perfusion apparatus or at least one shock experienced by the perfusion apparatus; and altering perfusion based upon the detected condition and/or recording the detected condition.

Abstract: An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a cooling container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment.

Abstract: A filter for filtering perfusate is integrated with an exterior portion of an organ container. The filter may be used in an apparatus for perfusing an organ. The perfusion apparatus may include an organ container configured to contain an organ, the filter integrated with an exterior portion of the organ container, and another filter. At least the filter integrated with an exterior portion of the organ container may be provided in a sterilized disposable kit.

Abstract: A cannula has a longitudinal base member having a first clamping end and a longitudinal arm member pivotally attached to the longitudinal base member and having a second clamping end, the cannula being in a closed position when the first and second clamping ends are brought together. A first clamp member is located at the first clamping end and has a first passage. A second clamp member is rotatably attached to the arm member at the second clamping end and has a second passage. The first and second passages form a combined passage when the clamp is in the closed position. The cannula may have a first locking member positioned on the arm member and a second locking member positioned on the base member. The first and second locking members interact with each other to lock the cannula in the closed position.

Abstract: An apparatus for separating gas bubbles that may be entrained in perfusate flow prevents such bubbles from continuing downstream and entering an organ or tissue. The apparatus may include a chamber having a top wall, a bottom wall and side walls. The chamber may include an inlet configured to allow at least one of gas and liquid to enter the chamber, an air opening configured to allow at least gas to exit the chamber and a first liquid opening configured to allow at least liquid to exit the chamber. The apparatus may function as an accumulator that reduces or eliminates pulsatility of the liquid flow and pressure. The apparatus may include a minimum volume of gas, initially or through the accumulation of gas, such that flow and pressure fluctuations in the liquid are dampened or eliminated. The apparatus may include a sampling port in a wall of the chamber.

Abstract: An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a cooling container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment.

Abstract: An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a cooling container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment.

Abstract: An apparatus for at least one of storage, treatment, assessment and transport of an organ or tissue includes a coolant container configured to cool the organ or tissue, a perfusion circuit configured to perfuse the organ or tissue, and a sample compartment for holding a biological sample. Preferred apparatus has a first internal compartment under a first cover (lid) of the apparatus that includes the coolant container and the sample compartment. The apparatus can include a second internal compartment under a second cover (lid) of the apparatus, the second internal compartment including at least part of the perfusion circuit and a sample compartment.