Abstract: An apparatus for in-situ sensing of longitudinal and transverse strain in biological tissue (e.g. tendons and ligaments) includes a set of sensing electrodes that contact biological tissue and enable measurement of an electrical property of the biological tissue along two or more directions. The apparatus may also include a sensing module that senses the electrical property of the biological tissue along the two or more directions to provide multi-directional measurements that are captured by a logging module. The apparatus may also include a transmission module that transmits the captured measurements to a data analysis workstation or the like. The data analysis workstation may include a strain estimation module that receives the multi-directional measurement and estimates a longitudinal strain and a transverse strain therefrom. A corresponding system and method for in-situ sensing of longitudinal and transverse strain in biological tissue is also presented.