Abstract: Systems and methods are provided for encoding and decoding images using differentiable JPEG compression, including converting images from RGB color space to YCbCr color space to obtain a luminance and chrominance channels, and applying chroma subsampling to the chrominance channels to reduce resolution. The YCbCr image is divided into pixel blocks and a DCT is performed on the pixel blocks to obtain DCT coefficients. DCT coefficients are quantized using a scaled quantization table to reduce precision, and quantized DCT coefficients are encoded using lossless entropy coding, forming a compressed JPEG file decoded by reversing the lossless entropy coding to obtain quantized DCT coefficients, which are dequantized using the scaled quantization table to restore the precision. The dequantized DCT coefficients are converted back to a spatial domain using an IDCT, the chrominance channels are upsampled to original resolution, and the YCbCr image is converted back to the RGB color space.

Abstract: Systems and methods are provided for optimizing video compression for remote vehicle control, including capturing, capturing video and sensor data from a vehicle using a plurality of sensors and high-resolution cameras, analyzing the captured video to identify critical regions within frames of the video using an attention-based module. Current network bandwidth is assessed and future bandwidth availability is predicted. Video compression parameters are predicted based on an analysis of the video and an assessment of the current network bandwidth using a control network, and the video is compressed based on the predicted parameters with an adaptive video compression module. The compressed video and sensor data is transmitted to a remote-control center, and received video and sensor data is decoded at the remote-control center. The vehicle is autonomously or remotely controlled from the remote-control center based on the decoded video and sensor data.

Abstract: Systems and methods are provided for optimizing video compression using end-to-end learning, including capturing, using an edge device, raw video frames from a video clip and determining maximum network bandwidth. Predicting, using a control network implemented on the edge device, optimal codec parameters, based on dynamic network conditions and content of the video clip, encoding, using a differentiable surrogate model of a video codec, the video clip using the predicted codec parameters and to propagate gradients from a server-side vision model to adjust the codec parameters. Decoding, using a server, the video clip and analyzing the video clip with a deep vision model located on the server, transmitting, using a feedback mechanism, analysis from the deep vision model back to the control network to facilitate end-to-end training of the system. Adjusting the encoding parameters based on the analysis from the deep vision model received from the feedback mechanism.

Abstract: The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.

Abstract: The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.

Abstract: Aspects of the present disclosure provide systems, methods, apparatus, and computer-readable storage media that support local and cloud-based tax determination operations. In some examples, a transaction system may execute a local tax engine to enable localized tax calculation services in a lightweight footprint while still achieving one or more benefits of a cloud-based system (e.g., technical support and automatic updates of software and tax content). As a result, some aspects of the present disclosure may enable a “full power” tax engine that is local to a transactional system, such as an edge device, while also enabling the tax engine to automatically sync back to a cloud-based system, such as to receive one or more of a configuration update, a software release, a software update, or updated tax content. Further, the transaction system may transmit results of tax calculations to the cloud-based system, such as for reporting, compliance, or both.

Abstract: The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.

Abstract: The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.

Abstract: The present invention provides a process for the recovery and/or purification of a recombinantly expressed intracellular protein comprising the sequence of Annexin A5 (AnxA5) from an endotoxin-producing host cell with a cell wall, wherein the process comprises releasing the intracellular protein from the host cell, characterised in that the step of releasing the intracellular AnxA5 protein is conducted in the presence of a homogenisation buffer comprising non-ionic detergent, and preferably wherein the process does not include any centrifugation steps for the recovery and/or purification of the AnxA5 protein after its release from the host cell and/or in which the AnxA5 protein remains in solution throughout the process except when temporarily bound to any chromatographic resins.

Abstract: Systems, methods, and sensor devices for identifying one or more changes in a stimulus-responsive hydrogel include a sensor device having (i) a sensing structure and (ii) a stimulus-responsive hydrogel associated with a first side of the sensing structure. The sensing structure includes a flexible thin film polymer and an electric sensing element capable of electric impedance change, and the hydrogel is configured to dimensionally change in response to predefined stimuli such that a dimensional change of the hydrogel causes a change in an impedance property of the electric sensing element. Systems including such a sensor device can additionally include a meter in electrical communication with the sensor device to identify changes in the impedance properties of the structure and/or a catheter sheath configured for placement within an in vivo environment and is sized and shaped to receive the sensor device within a lumen thereof.

Abstract: The present invention relates to a novel method for purifying teriparatide, a therapeutically active polypeptide fragment of full-length human parathyroid hormone. The method is based on ion exchange chromatography and is effective in separating the therapeutically active polypeptide from undesirable variants, such as truncated polypeptides. The method of the invention can be used at a preparative scale which allows it to be implemented into the production process for teriparatide. Accordingly, the invention also provides a method for the production of teriparatide which includes a step in which teriparatide is purified by the novel ion exchange chromatography method of the invention.

Abstract: The present invention relates to a novel method for purifying teriparatide, a therapeutically active polypeptide fragment of full-length human parathyroid hormone. The method is based on ion exchange chromatography and is effective in separating the therapeutically active polypeptide from undesirable variants, such as truncated polypeptides. The method of the invention can be used at a preparative scale which allows it to be implemented into the production process for teriparatide. Accordingly, the invention also provides a method for the production of teriparatide which includes a step in which teriparatide is purified by the novel ion exchange chromatography method of the invention.