Abstract: The present invention relates to a method for producing at least one polypeptide of interest in a Bacillus host cell, said method comprising the steps of a) providing a Bacillus host cell comprising a polynucleotide encoding the at least one polypeptide of interest, b) cultivating the Bacillus host cell under conditions which allow for intracellular accumulation of said at least one polypeptide of interest, thereby producing said at least one polypeptide of interest, c) stop of aeration and addition of feed, and d) incubating the cells for at least hours. Moreover, the present invention relates to a Bacillus host cell comprising a polynucleotide encoding at least one polypeptide of interest lacking a functional secretion sequence, wherein the ispA gene has been inactivated in the Bacillus host cell.

Abstract: The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications that lead to decreased production of components of biofilm and increased levels of phosphorylated De-gll. Said Bacillus host comprises at least one gene expression cassette with a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter for the production of compounds. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: The present invention relates to a Bacillus licheniformis host cell for production of biological compounds with increased purity. Specifically, the invention relates to a Bacillus licheniformis host with one or more genetic modifications selected from a) a genetic modification causing compared to an unmodified control cell an increased expression of at least one of the genes selected from the group consisting of degQ, degll, degS, degR, and phrG, b) a genetic modification causing compared to an unmodified control cell an increased autophosphorylation activity of the DegS protein, and c) a genetic modification causing compared to an unmodified control cell a reduced phosphatase activity of the DegS protein. The present invention further relates to a method for production of at least one compound of interest with increased purity based on cultivating the bacterial host cell of the present invention.

Abstract: The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications in the remA and/or remB gene. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.

Abstract: The present invention relates to a bacterial host cell in which a first chromosomal gene encoding a first alanine racemase and a second chromosomal gene encoding a second alanine racemase have been inactivated. Said bacterial host cell comprises – either on a plasmid comprising at least one autonomous replication sequence or present as multiple copies in the chromosome – a gene expression cassette comprising a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter, and a polynucleotide encoding a third alanine racemase, operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: The present invention relates to a Bacillus host cell belonging to the species Bacillus lichemformis or Bacillus pumilus in which the chromosomal alr gene has been inactivated. Said bacterial host cell comprises a plasmid comprising at least one autonomous replication sequence, a first polynucleotide encoding at least one polypeptide of interest, wherein said first polynucleotide is operably linked to a promoter, and a second polynucleotide encoding an alanine racemase which is not native to the host cell, wherein said second polynucleotide is operably linked to a promoter. The present invention further relates to a method for producing at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

Abstract: Described herein are materials and methods for user-controlled adjustment of plasmid copy numbers.

Abstract: Disclosed herein are methods for the production of low to medium expressing constitutive promoters in bacteria and promoters produced therewith.

Abstract: Disclosed herein is a shuttle vector for use in E. coli and Bacilli including a high copy replication origin functional in E. coli, a low to medium copy ORI functional in Bacilli, and a synthetic constitutive regulatory nucleic acid conferring reduced constitutive expression compared to a respective starting regulatory nucleic acid molecule in a bacterial cell.

Abstract: An assembly, by way of example, an aircraft, including an aircraft motor apparatus including at least one motor located within a protective structure, the protective structure having movable sub-structure components, wherein the portion of the aircraft motor apparatus is configured to move the movable sub-structure components to increase or decrease airflow through the protective structure.

Abstract: The present invention is directed to a turbine comprising a pair of opposing end discs concentrically aligned with a central axis of the turbine and a plurality of blades extending between the end discs. At least one end discs is adapted for engaging with a generator for generating power. The plurality of blades rotates in a single direction when exposed to fluid flow and thereby rotates the pair of opposing end discs. The plurality of blades are interconnected by at least one faired ring oriented parallel to the pair of opposing end discs and intersecting the plurality of blades, wherein the at least one faired ring is in concentric alignment with the central axis. The present invention further comprises a method for generating power comprising engaging the turbine with a generator to create a turbine generator unit and deploying the turbine generator unit within a fluid flow.

Abstract: The present invention is directed to a turbine comprising a pair of opposing end discs concentrically aligned with a central axis of the turbine and a plurality of blades extending between the end discs. At least one end discs is adapted for engaging with a generator for generating power. The plurality of blades rotates in a single direction when exposed to fluid flow and thereby rotates the pair of opposing end discs. The plurality of blades are interconnected by at least one faired ring oriented parallel to the pair of opposing end discs and intersecting the plurality of blades, wherein the at least one faired ring is in concentric alignment with the central axis. The present invention further comprises a method for generating power comprising engaging the turbine with a generator to create a turbine generator unit and deploying the turbine generator unit within a fluid flow.